Rap GTPase-mediated adhesion and migration

Lin, Kevin B.L.; Freeman, Spencer A.; Gold, Michael R.

doi:10.4161/cam.4.3.11114

Cited by 10 publications

(3 citation statements)

References 46 publications

(54 reference statements)

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“…Moreover, Rap GTPases are master regulators of integrin activation, cell motility, and the underlying cytoskeletal, adhesion, and membrane dynamics. Rap activation is critical for B-lymphoma cells to undergo transendothelial migration in vitro and in vivo [ 25 ]. In addition, altered expression of hsa-miR-337-3p may be critical in renal cell carcinoma (RCC) development, although the analysis of circulating serum levels of hsa-miR-337-3p is unlikely to provide helpful diagnostic/prognostic information in RCC [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer

Wang

Yang

et al. 2013

J Exp Clin Cancer Res

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BackgroundMetastasis is the major cause of cancer-related death in patients with gastric cancer, and aberrant expression of various microRNAs (miRNAs) is associated with cancer metastasis.MethodsProfiling of differentially expressed miRNAs was performed in three cases of primary gastric cancer and the corresponding metastatic lymph node tissues. Then, the five most altered miRNAs were further verified in 16 paired samples. Two of these five miRNAs were further assessed for their effects on the regulation of gastric cancer cell growth and invasion.ResultsThe miRNA profile data showed 151 upregulated miRNAs (≥ 1.5-fold) and 285 downregulated miRNAs (≤ 0.67-fold) in the metastatic tissues compared to the primary gastric cancer tissues. Among these five miRNAs (i.e., hsa-miR-508-5p, hsa-miR-30c, hsa-miR-337-3p, hsa-miR-483-5p, and hsa-miR-134), expression of hsa-miR-337-3p and hsa-miR-134 was significantly downregulated in these 16 lymph node metastatic tissues compared to their primary tumor tissues (P<0.05) and in nine gastric cancer cell lines compared to the nonmalignant GES cell line. Furthermore, induction of hsa-miR-134 or hsa-miR-337-3p expression did not dramatically affect gastric cancer cell proliferation, but transfection of the hsa-miR-337-3p mimic did reduce gastric cancer cell invasion capacity.ConclusionsThese findings indicate that hsa-miR-337-3p plays a role in the reduction of gastric cancer cell invasion capacity, and further studies on the mechanism of hsa-miR-337-3p in gastric cancer metastasis are warranted.

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Section: Discussionmentioning

confidence: 99%

Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer

Wang

Yang

et al. 2013

J Exp Clin Cancer Res

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“…Rap GTPase and TNIK are well-known actin cytoskeleton regulators ( Lin et al, 2010 , 2008 ; Taira et al, 2004 ). Previous studies demonstrated that presynaptic development requires ARP2/3-dependent branched F-actin ( Chia et al, 2012 , 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans

Chen

Shibata

Hendi

et al. 2018

eLife

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During development, neurons form synapses with their fate-determined targets. While we begin to elucidate the mechanisms by which extracellular ligand-receptor interactions enhance synapse specificity by inhibiting synaptogenesis, our knowledge about their intracellular mechanisms remains limited. Here we show that Rap2 GTPase (rap-2) and its effector, TNIK (mig-15), act genetically downstream of Plexin (plx-1) to restrict presynaptic assembly and to form tiled synaptic innervation in C. elegans. Both constitutively GTP- and GDP-forms of rap-2 mutants exhibit synaptic tiling defects as plx-1 mutants, suggesting that cycling of the RAP-2 nucleotide state is critical for synapse inhibition. Consistently, PLX-1 suppresses local RAP-2 activity. Excessive ectopic synapse formation in mig-15 mutants causes a severe synaptic tiling defect. Conversely, overexpression of mig-15 strongly inhibited synapse formation, suggesting that mig-15 is a negative regulator of synapse formation. These results reveal that subcellular regulation of small GTPase activity by Plexin shapes proper synapse patterning in vivo.

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“…Rap GTPase and TNIK are well-known actin cytoskeleton regulators (Lin et al, 2010;Taira et al, 2004). Previous studies demonstrated that ARP2/3-dependent branched F-actin is required for presynaptic development (P. Chia et al, 2012;P.…”

Section: Mig-15 Inhibits Synapse Formationmentioning

confidence: 99%

Rap2 and TNIK control Plexin-dependent synaptic tiling in C. elegans

Chen

Shibata

Weilinger

et al. 2017

Preprint

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During development, neurons form extensive synaptic connectivity with their fate-determined targets. Ectopic synapse formation with aberrant targets underlie various neurological disorders including autism. While we are beginning to elucidate the underlying mechanisms by which extracellular ligand-receptor interactions enhance synapse specificity by inhibiting synaptogenesis, our knowledge about their intracellular mechanisms remains limited. Here we show that Rap2GTPase (rap-2) and its effector, TNIK (mig-15), act downstream of Plexin (plx-1) signaling to restrict presynaptic assembly and to form tiled synaptic innervation of two cholinergic motor neurons (DA8 and DA9) in C. elegans. Both constitutively GTP-and GDP-forms of rap-2 mutants exhibit similar synaptic tiling defects as plx-1 mutants, suggesting that cycling of the RAP-2 nucleotide state is critical for synapse inhibition. Consistently, RAP-2 activity is suppressed in a short segment of axon lacking synapses where PLX-1 is enriched. Excessive ectopic synapse formation in mig-15 mutants causes expansion of the synaptic domains in DA8 and DA9, which induces a severe synaptic tiling defect. Conversely, overexpression of mig-15 strongly inhibited synapse formation, which suggests that mig-15 is a negative regulator of synapse formation. These results reveal that subcellular regulation of small GTPase activity by Plexin shapes proper synapse patterning in vivo.peer-reviewed)

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Rap GTPase-mediated adhesion and migration

Cited by 10 publications

References 46 publications

Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer

Loss of has-miR-337-3p expression is associated with lymph node metastasis of human gastric cancer

Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans

Rap2 and TNIK control Plexin-dependent synaptic tiling in C. elegans

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