RAPD analysis of selected local Turkish grape cultivars (Vitis vinifera)

Abstract

ABSTRACT. Turkey is very rich in local grape varieties. The solution to the problem of identifying local cultivars, which is considered an important deficiency for the region, will only be possible when they can be defined with molecular markers. Forty-nine local grapevine cultivars from Şanlıurfa (Turkey) were characterized with RAPD markers. Twenty-five decamer primers selected from 60 primers were used in this analysis. A total of 171 bands were obtained with the 25 primers, of which 112 were polymorphic; the level of DNA polymorphism was 65.49% in these local cultivars. Among the selected primers, OPA-18, OPO-07 and P-123 gave the maximum number of polymorphic bands (seven). Genetic relationships among these cultivars were determined with a similarity index and using a dendogram. Among the grape cultivars, the lowest similarity ratio (0.578) was observed among the Külahi-Kızılbanki cultivars and the highest similarity ratio (0.908) was observed for the Çilorut-Dökülgen combination. The high similarity ratio among the grape cultivars of Şanlıurfa Province was also reflected in the dendogram. 1981©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (4): 1980-1986 (2010) RAPD analysis of Turkish grape cultivars (Vitis vinifera) In general, no relationships were encountered between the genetic identification of the cultivars and their ampelographic properties.

Genetic diversity of hybrid durian resulted from cross breeding between <i>Durio kutejensis</i> and <i>Durio zibethinus</i> based on random amplified polymorphic DNAs (RAPDs)

“…Compared to other molecular markers, RAPD is easy to be conducted, fast, requiring a small amount of DNA (0.5 -50 ng), does not require radioisotopes and does not require prior information of DNA sequence of the sample [9,10]. This technique has been widely used to analyze genetic diversity in crops such as citrus, sugarcane, durian sukun, grape, potato, soybean, kenaf, warty cabbage and cotton [9][10][11][12][13][14][15][16][17].…”

Development of Sequence-characterized Amplified Region Markers for the Identification of Grapevine Cultivars

“…The lack of reproducibility is a pitfall both to cultivar identification in routine procedures and to data exchange among laboratories (B€ uscher et al, 1993). Despite a number of drawbacks, RAPD markers are still widely used for identifying cultivars and clones and for genetic analysis (El-Sayed et al, 2011;Karatas and A gao glu, 2010;Zhao et al, 2011). Zhao et al (2011) created a cultivar identification diagram of 69 grapevine cultivars obtained with seven RAPD markers after they confirmed the reproducibility of the analytic conditions.…”

Biotechnologies and Strategies for Grapevine Improvement

“…Since their discovery, different molecular markers, such as restriction fragment length polymorphisms (RFLPs) [45], random amplified polymorphic DNA (RAPD) [46], sequence characterized amplified regions (SCARs) [47,48], simple sequence repeats (SSRs) [31,49], inter-simple sequence repeats (ISSRs) [50,51], amplified fragment length polymorphisms (AFLPs) [52], single nucleotide polymorphisms (SNPs) [53], expressed sequence tags (ESTs) [54,55], and random amplified microsatellite polymorphisms (RAMPs) [56] have been widely used for the genetic diversity characterization of grapevine cultivars, molecular mapping, parentage analysis, clone's identification, and the detection of synonymies.…”