Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays

Liu, Huiyu; Kumar, Ravi; Zhong, Chunting; Gorji, Mohammad Saleh; Paniushkina, Liliia; Masood, Ramsha; Wittel, Uwe A.; Fuchs, Harald; Nazarenko, Irina; Hirtz, Michael

doi:10.1002/adma.202008493

Cited by 51 publications

(58 citation statements)

References 86 publications

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“…Cancer immunotherapy has emerged as a promising alternative to conventional therapies to treat a variety of malignancies and has demonstrated remarkable clinical results. Immune cell-derived EVs are gaining considerable attention as potential cancer treatment candidates ( 142 ). At present, numerous studies have focused on EVs, and their structure, formation, secretion, and functions have uncovered a significant role of EVs as intercellular communication messengers ( 36 , 39 ).…”

Section: Discussionmentioning

confidence: 99%

Immune Cell-Derived Extracellular Vesicles – New Strategies in Cancer Immunotherapy

et al. 2021

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Immune cell-derived extracellular vesicles (EVs) have increasingly become the focus of research due to their unique characteristics and bioinspired applications. They are lipid bilayer membrane nanosized vesicles harboring a range of immune cell-derived surface receptors and effector molecules from parental cells. Immune cell-derived EVs are important mediators of intercellular communication that regulate specific mechanisms of adaptive and innate immune responses. However, the mechanisms underlying the antitumor effects of EVs are still being explored. Importantly, immune cell-derived EVs have some unique features, including accessibility, storage, ability to pass through blood-brain and blood-tumor barriers, and loading of various effector molecules. Immune cell-derived EVs have been directly applied or engineered as potent antitumor vaccines or for the diagnosis of clinical diseases. More research applications involving genetic engineering, membrane engineering, and cargo delivery strategies have improved the treatment efficacy of EVs. Immune cell-derived EV-based therapies are expected to become a separate technique or to complement immunotherapy, radiotherapy, chemotherapy and other therapeutic modalities. This review aims to provide a comprehensive overview of the characteristics and functions of immune cell-derived EVs derived from adaptive (CD4+ T, CD8+ T and B cells) and innate immune cells (macrophages, NK cells, DCs, and neutrophils) and discuss emerging therapeutic opportunities and prospects in cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Immune Cell-Derived Extracellular Vesicles – New Strategies in Cancer Immunotherapy

et al. 2021

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“…(B) Scheme of lipid membranes microarrays functionalized with antibodies. Adapted from ( Liu H. Y. et al, 2021 ), copyright 2021 Wiley-VCH Verlag GmbH & Co. (C) 3D nanopatterned EV-CLUE chip were manufactured by colloidal inkjet printing. Adapted from ( Zhang et al, 2020 ), copyright 2020 American Association for the Advancement of Science.…”

Section: Emerging Separation Techniquementioning

confidence: 99%

“…Liu et al established supported lipid membranes microarrays functionalized with antibodies for recognizing and capturing cancer-specific exosomes ( Figure 7B ) ( Liu H. Y. et al, 2021 ). In this research, the lipid microarray was first structured using lipid dip-pen nanolithography, followed by incubating and self-assembling onto the lipid microarrays by using Biotin-PE, streptavidin, and specific biotinylated antibodies in a prearranged sequence.…”

Section: Emerging Separation Techniquementioning

confidence: 99%

Review on Strategies and Technologies for Exosome Isolation and Purification

Chen

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

295

166

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Exosomes, a nano-sized subtype of extracellular vesicles secreted from almost all living cells, are capable of transferring cell-specific constituents of the source cell to the recipient cell. Cumulative evidence has revealed exosomes play an irreplaceable role in prognostic, diagnostic, and even therapeutic aspects. A method that can efficiently provide intact and pure exosomes samples is the first step to both exosome-based liquid biopsies and therapeutics. Unfortunately, common exosomal separation techniques suffer from operation complexity, time consumption, large sample volumes and low purity, posing significant challenges for exosomal downstream analysis. Efficient, simple, and affordable methods to isolate exosomes are crucial to carrying out relevant researches. In the last decade, emerging technologies, especially microfluidic chips, have proposed superior strategies for exosome isolation and exhibited fascinating performances. While many excellent reviews have overviewed various methods, a compressive review including updated/improved methods for exosomal isolation is indispensable. Herein, we first overview exosomal properties, biogenesis, contents, and functions. Then, we briefly outline the conventional technologies and discuss the challenges of clinical applications of these technologies. Finally, we review emerging exosomal isolation strategies and large-scale GMP production of engineered exosomes to open up future perspectives of next-generation Exo-devices for cancer diagnosis and treatment.

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“…This principle has also been applied to the isolation and content analysis of exosomes ( Ning et al, 2021 ). Liu et al designed an antibody-modified lipid patch microarray for rapid capture of cancer extracellular vesicles ( Liu Hui-Yu et al, 2021 ). The binding exosomes successfully fused with the lipid layer to trap the contents to the surface of the microarray.…”

Section: Isolation and Enrichment Strategies Of Exosomesmentioning

confidence: 99%

Recent Progress of Exosome Isolation and Peptide Recognition-Guided Strategies for Exosome Research

Jin

et al. 2022

Front. Chem.

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Exosomes are membrane extracellular vesicles secreted by almost all kinds of cells, which are rich in proteins, lipids, and nucleic acids. As a medium of intercellular communication, exosomes play important roles in biological processes and are closely related to the occurrence, and development of many diseases. The isolation of exosomes and downstream analyses can provide important information to the accurate diagnosis and treatment of diseases. However, exosomes are various in a size range from 30 to 200 nm and exist in complex bio-systems, which provide significant challenges for the isolation and enrichment of exosomes. Different methods have been developed to isolate exosomes, such as the “gold-standard” ultracentrifugation, size-exclusion chromatography, and polymer precipitation. In order to improve the selectivity of isolation, affinity capture strategies based on molecular recognition are becoming attractive. In this review, we introduced the main strategies for exosome isolation and enrichment, and compared their strengths and limitations. Furthermore, combined with the excellent performance of targeted peptides, we summarized the application of peptide recognition in exosome isolation and engineering modification.

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Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays

Cited by 51 publications

References 86 publications

Immune Cell-Derived Extracellular Vesicles – New Strategies in Cancer Immunotherapy

Immune Cell-Derived Extracellular Vesicles – New Strategies in Cancer Immunotherapy

Review on Strategies and Technologies for Exosome Isolation and Purification

Recent Progress of Exosome Isolation and Peptide Recognition-Guided Strategies for Exosome Research

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