Rapid detection of multiple foodborne pathogens using a nanoparticle-functionalized multi-junction biosensor

Yamada, Kara; Choi, Won Tae; Lee, Inae; Cho, Byoung‐Kwan; Jun, Soojin

doi:10.1016/j.bios.2015.09.030

Cited by 66 publications

(33 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The integration of nanomaterials in biosensors has provided significant improvements in terms of selectivity and analysis time of biosensing methods . Considering the type of nanomaterial for sensor applications is crucial, as most of these materials are rather costly or require lengthy synthesis procedures.…”

Section: Discussionmentioning

confidence: 99%

“…An example for the efficient simultaneous detection of pathogens is the research by Yamada et al., which is based on a multi‐junction array system. The authors report a single step bacterial detection method, using a single walled carbon nanotube‐ (SWCNT) based multi‐junction sensor . Four SWCNT coated wires were placed and soldered onto each disposable sensor chip device, to create a 2×2 junction array.…”

Section: Literature Reviewmentioning

confidence: 99%

“…The authors report as ingle step bacterialdetection method, using asingle walled carbon nanotube-(SWCNT) based multi-junction sensor. [36] Four SWCNT coated wires were placed and soldered onto each disposable sensor chip device,t oc reate a2 2j unction array.B acteria specific antibodies were immobilized onto each junction,created by the SWCNT coated wires. Ac hange in current was ob- Figure 1.…”

Section: Carbonnanomaterialsmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Kuss

Amin

Compton

2018

Chemistry An Asian Journal

View full text Add to dashboard Cite

Bacterial infections represent one of the leading causes of mortality worldwide, nevertheless the design and development of rapid, cost-efficient and reliable detection methods for pathogens remains challenging. In recent years, electrochemical sensing methods have gained increasing attention for the detection of pathogenic bacteria, due to their increasingly competitive sensitivity. However, combining sensitivity with cost efficiency, high selectivity and a facile working procedure in a portable device is difficult. The presented review provides a summary of biosensing strategies for bacteria, published since 2015, by covering significant achievements towards custom-designed portable point-of-care devices. Herein, the direct chemical recognition of bacteria via enzyme activity or secretion products, as well as their detection at various electrode surfaces and materials, such as nanomaterials, indium tin oxide or paper-based immunosensors, is discussed. Furthermore, newly established hyphenated sensing principles, incorporated into lab-on-a-chip and microfluidic devices, are presented and remaining technical challenges and limitations are considered.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Literature Reviewmentioning

confidence: 99%

Section: Carbonnanomaterialsmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Kuss

Amin

Compton

2018

Chemistry An Asian Journal

View full text Add to dashboard Cite

show abstract

“…Both the security and safety of food products rely on the ability to detect identify and trace foodborne pathogens [1,2]. Foodborne pathogens detection methods have a great significance due to their biological threats to public health and economy.…”

Section: Introductionmentioning

confidence: 99%

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

Evrendilek¹,

Richter²

2017

J Food Microbiol Saf Hyg

View full text Add to dashboard Cite

Rapid and specific identification of foodborne pathogens is important for not only for food producers but also consumers as well as food safety authorities. Foods can harbor pathogen bacteria of different species, thus it is also important to be able to detect different pathogens simultaneously from the food samples even though they are in very low number. Detection of bacteria depends on different factors such as the initial number, food matrix, sensitivity of the method, competitor microflora and physical state of the bacteria whether they are injured or not. Simultaneous detection of different bacteria yet adds another hurdle for the level of detection, and thus, different approaches have developed and tested so far to optimize the maximum recovery.

show abstract

“…The biosensor exhibited the selectivity against E. coli K12 and MS bacteriophage (García‐Aljaro and others ). Bio‐nano combinational junction sensor with functionalized SWCNTs has shown the potential for high‐performance biosensing to detect foodborne pathogens (Yamada and others , ). The junction sensor works to convert bioaffinity binding between target bacteria cell and antibodies into measurable electrical current signals (Figure A).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of E. coli K12 and S. aureus Using a Continuous Flow Multijunction Biosensor

Lee

Jun

2016

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

Rapid detection and identification of potentially harmful bacteria is ideal for food manufacturers to prevent foodborne illness outbreaks. Continuous monitoring method of foodborne pathogens levels and trends in food gives real-time results. Therefore, the objectives of this study were to fabricate and characterize the continuous flow multijunction biosensor for simultaneous detection of Escherichia coli K12 and Staphylococcus aureus. Junction biosensors were fabricated using gold plated tungsten wires coated with polyethylenimine and single walled carbon nanotubes. Each junction was functionalized with streptavidin and biotinylated antibodies specific to E. coli K12 and S. aureus. Then, single or 2 biosensors for each targeted analyte were connected to tubing, perpendicular to the flow direction. Pure serial diluted samples of E. coli K12 and S. aureus and microbial cocktail samples were continuously pumped at a 0.0167 mL/s into the detection zone. Changes in the electric current by biorecognition reactions between antibody and antigens were calculated. The developed junction sensor coupled with the fluidic channel showed the enhancement of the electric signal responses for detection of E. coli K12, compared to the stationary sensor. A linear regression was observed for both the E. coli and S. aureus functionalized array sensors in the detection range of 10(2) to 10(5) CFU/mL. Multiplexed detection of bacteria at the sensing levels as low as 10(2) CFU/mL for E. coli K12 and S. aureus was achieved within 2 min. Therefore, the continuous flow multijunction biosensor shows potential for rapid and continuous multiplexed detection of foodborne pathogens.

show abstract

Rapid detection of multiple foodborne pathogens using a nanoparticle-functionalized multi-junction biosensor

Cited by 66 publications

References 37 publications

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Concurrent Detection of Foodborne Pathogens: Past Efforts and Recent Trends

Simultaneous detection of E. coli K12 and S. aureus Using a Continuous Flow Multijunction Biosensor

Contact Info

Product

Resources

About