Rapid determination of lead and cadmium in biological fluids by electrothermal atomic absorption spectrometry using Zeeman correction

Campillo, Natalia; Viñas, Pilar; López-Garcı́a, Ignacio; Hernández‐Córdoba, Manuel

doi:10.1016/s0003-2670(99)00166-x

Cited by 42 publications

(30 citation statements)

References 28 publications

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“…The determination of human risk from toxic metal exposure is usually done through the determination of metals in biological samples such as blood, serum, urine, hair, fingernails, and saliva (Campillo et al 1999;Lemos et al 2009). The continuous exposure to low levels of cadmium and lead can result in bioaccumulation and negative health effects (Kummrow et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Determination of cadmium and lead in human biological samples by spectrometric techniques: a review

Lemos

Carvalho

2009

Environ Monit Assess

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The analysis of human biological samples, such as blood, urine, nails, and hair, is generally used for the verification of human exposure to toxic metals. In this review, various spectrometric methods for the determination of cadmium and lead in biological samples are discussed and compared. Several spectrometric techniques are presented and discussed with respect to various characteristics such as sensitivity, selectivity, and cost. Special attention is drawn to the procedures for digestion prior to the determination of cadmium and lead in hair, nails, blood, and urine.

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Section: Introductionmentioning

confidence: 99%

Determination of cadmium and lead in human biological samples by spectrometric techniques: a review

Lemos

Carvalho

2009

Environ Monit Assess

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“…The addition of nitric acid or nickel salt to the blood and serum samples produced protein precipitation and, consequently, two successive aliquots of 10 l, separated by a drying step, were injected using the autosampler in the dry last method as previously described (16). The first injection was of the sample diluted with a Triton X-100 solution (dilution 1 ϩ 1 for urine and 1 ϩ 3 for serum or blood) being the final concentration of this chemical 0.1% w/v.…”

Section: Methodsmentioning

confidence: 99%

Selenium Determination in Biological Fluids Using Zeeman Background Correction Electrothermal Atomic Absorption Spectrometry

Campillo

Viñas

López-Garcı́a

et al. 2000

Analytical Biochemistry

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“…The ideal methods would require minimal sample handling and have low detection limits. Among the various spectrometric techniques available for the determination of cadmium, electrothermal atomic absorption spectrometry (ETAAS) [5][6][7][8] and inductively coupled plasma mass spectrometry (ICP-MS) [9][10][11] are the most commonly used techniques, because of their high sensitivities, excellent detection limit and easy sample handling. However, either the cost of the commercial instruments or their operations may go beyond the acceptable limit for large scale screening in clinical laboratories.…”

Section: Introductionmentioning

confidence: 99%

Rapid and low cost determination of cadmium in whole blood by hydride generation atomic fluorescence spectrometry after ultrasound-assisted sample preparation

Li¹,

Zhou

2008

J. Braz. Chem. Soc.

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Foi desenvolvido um método rápido, de baixo custo e preciso para a determinação de cádmio em sangue integral usando espectrometria de fluorescência atômica com geração de hidreto (HG-AFS). A amostra foi preparada por lixiviação assistida por ultra-som e a solução resultante foi usada diretamente para a determinação do cádmio por HG-AFS. Adotando-se as condições otimizadas para o procedimento proposto, o limite de detecção (LOD) e limite de quantificação (LOQ) foram de 0,08 e 0,26 µg L -1 , respectivamente, calculados a partir de três e dez vezes o desvio padrão de onze medidas para a amostra de sangue bovino (branco), com baixo teor de Cd (0,2 µg L -1 , diluída por um fator de 10). O desvio-padrão relativo (RSD, n = 7) variou entre 3-10% (entre medidas) para amostras de sangue contendo de 1 a 8 µg L -1 de cádmio. A precisão e aplicabilidade prática do método foram comprovadas analisando-se materiais de referência certificados (MRCs). Os resultados foram ainda comparados com aqueles obtidos por espectrometria de massa com fonte de plasma indutivamente acoplado (ICP-MS). Uma boa concordância foi observada entre os resultados obtidos por HG-AFS e ICP-MS para a determinação de cádmio em MRCs e em amostras de sangue humano.A rapid, low cost and precise method was developed for the determination of cadmium in whole blood using hydride generation atomic fluorescence (HG-AFS). Sample was simply prepared utilizing ultrasound-assisted leaching and the resulting solution was directly used for cadmium determination by HG-AFS. Adopting optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) of the proposed procedure were 0.08 and 0.26 µg L −1 , calculated from three and ten times the standard deviations of eleven runs of blank blood sample (bovine blood) with low Cd content (0.2 µg L −1 Cd, tenfold diluted); and the relative standard deviation (RSD, n = 7) varied between 3-10% (between-run) at 1-8 µg L −1 cadmium level in blood. The accuracy and practical applicability of the method were validated by analyzing certified reference materials (CRMs) and further demonstrated by results comparison with inductively coupled plasma masss spectrometry (ICP-MS). Good agreement has been demonstrated between results obtained by HG-AFS and ICP-MS for cadmium determination in CRMS and human whole blood.

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Rapid determination of lead and cadmium in biological fluids by electrothermal atomic absorption spectrometry using Zeeman correction

Cited by 42 publications

References 28 publications

Determination of cadmium and lead in human biological samples by spectrometric techniques: a review

Determination of cadmium and lead in human biological samples by spectrometric techniques: a review

Selenium Determination in Biological Fluids Using Zeeman Background Correction Electrothermal Atomic Absorption Spectrometry

Rapid and low cost determination of cadmium in whole blood by hydride generation atomic fluorescence spectrometry after ultrasound-assisted sample preparation

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