Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives

García‐Ruiz, Carmen; Martı́n-Biosca, Yolanda; Crego, Antonio L.; Marina, Marı́a Luisa

doi:10.1016/s0021-9673(00)01176-6

Cited by 33 publications

(26 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At this respect, the use of cyclodextrin derivatives has shown enormous possibilities for the enantiomeric separation of compounds of pharmaceutical and environmental interest by CE [6,8,9]. Some possibilities of the use of cyclodextrin derivatives on the enantiomeric separation of compounds of environmental interest by CE have also been shown previously in different works of our research team [19][20][21].…”

Section: Introductionsupporting

confidence: 53%

Enantiomeric separation of organophosphorus pesticides by capillary electrophoresis

García-Ruiz

Álvarez-Llamas

Puerta

et al. 2005

Analytica Chimica Acta

View full text Add to dashboard Cite

The separation of the enantiomers of a group of organophosphorus pesticides (OPs) has been investigated by electrokinetic chromatography (EKC) using different anionic cyclodextrins as chiral selectors. The use of a 25 mM Tris buffer (pH 7.0), 20 mM in CM-␤-CD together with an applied voltage of 24 kV and a temperature of 25• C enabled the individual enantiomeric separation of malathion and phenthoate each one into its two enantiomers, the partial separation of the enantiomers of phenamiphos and the separation of three of the four enantiomers of isomalathion. Since naled is very reactive in aqueous solutions, its enantiomeric separation achieved in about 8 min in 25 mM borate buffer at pH 9 with 10 mM CM-␤-CD resulted in the observation of a broad peak due to degradation products. A preconcentration step by disk solid-phase extraction was studied and used together with the chiral EKC method developed for the enantiomeric separation of malathion in order to determine this pesticide in tap water samples spiked at the g mL −1 level. Finally, the precision and recovery of the method was established.

show abstract

Section: Introductionsupporting

confidence: 53%

Enantiomeric separation of organophosphorus pesticides by capillary electrophoresis

García-Ruiz

Álvarez-Llamas

Puerta

et al. 2005

Analytica Chimica Acta

View full text Add to dashboard Cite

show abstract

“…Chiral separation of atropisomeric PCBs by MEKC was first reported by Marina et al [72,73] In a recent work by García-Ruiz et al [67], the biodegradation of several chiral PCBs (45, 88, 91, 95, 136, 144, 149, and 176) by Jonibacter sp., a naturally occurring bacterium isolated from a contaminated soil, was studied. For this purpose, CZE was used together with one of the dual CD buffers optimized in a previous work [68] that consists of 50 mM MES at pH 6.5 containing 2 M urea, 20 mM CM-g-CD, and 10 mM b-CD. The study of the biodegradation process was carried out with mixtures of two congeners at a time (149-95, 88-136, 176-144, and 91-45).…”

Section: Chiral Analysis Of Pcbsmentioning

confidence: 99%

Chiral analysis of pollutants and their metabolites by capillary electromigration methods

et al. 2005

View full text Add to dashboard Cite

Chiral analysis of pollutants and their metabolites by capillary electromigration methodsChiral separation of enantiomers is one of the most challenging tasks for any analytical technique including CE. Since the first report in 1985 showing the great possibilities of CE for the separation of chiral compounds, the amount of publications concerning this topic has quickly increased. Although chiral electromigration methods have mainly been used for enantioseparation of drugs and pharmaceuticals, they have also been applied to analyze chiral pollutants. This article intends to provide an updated overview, including works published till January 2005, on the principal applications of CE to the chiral analysis of pollutants and their metabolites, with especial emphasis on articles published in the last 10 years. The main advantages and drawbacks regarding the use of CE for chiral separation of pollutants are addressed including some discussion on the foreseen trends of electromigration procedures applied to chiral analysis of contaminants. IntroductionSeparation of chiral compounds is an interesting and challenging topic of research in many analytical chemistry areas, especially in the biomedical, pharmaceutical, and environmental fields where pure enantiomeric forms are widely required. It is already well-known that enantiomers, in spite of their very similar structure, when exposed to an identical biological environment can show very different biological activity. Furthermore, as a general rule, when the toxicological effect of an active substance is directly related to its desired biological activity, the most active isomer is usually the most toxic one Abbreviations: Allyl-TER, 1-allylterguride; ANDSA, 7-aminonaphthalene-1,3-disulfonic acid; ANSA, 5-aminonaphthalene-1-sulfonic acid; ANTS, 8-aminonaphthalene-1,3,6-trisulfonic acid; CA, chrysanthemic acid; CM-ª-CD, carboxymethylated-g-CD; CMBA, 2-(4-chlorophenyl)-3-methylbutanoic acid; 2,2-CPPA, 2-(2-chlorophenoxy)-propionic acid; 2,3-CPPA, 2-(3-chlorophenoxy)-propionic acid; 2,4-CPPA, 2-(4-chlorophenoxy)-propionic acid; â-CD-NH 2 , 6-monodeoxy-6-monoamino-b-CD; â-CD- (NH 3 )

show abstract

“…In order to improve peak shape and resolution, 1 M urea was added to 15 mM CM-b-CD in 100 mM borate buffer at pH 9.0. In fact, urea has previously been shown to improve peak shape for other neutral aromatic compounds probably because it enables the better solubilization of them and CDs in the aqueous buffer [28]. However, in the case of MBC enantiomers the addition of urea did not improve the enantiomeric resolution.…”

Section: Development Of An Enantioselective Analytical Methods For Mbcmentioning

confidence: 97%

Enantioselective separation of the sunscreen agent 3-(4-methylbenzylidene)-camphor by electrokinetic chromatography: Quantitative analysis in cosmetic formulations

2005

View full text Add to dashboard Cite

Enantioselective separation of the sunscreen agent 3-(4-methylbenzylidene)-camphor by electrokinetic chromatography: Quantitative analysis in cosmetic formulations3-(4-Methylbenzylidene)-camphor (MBC) is a chiral sunscreen agent used in cosmetic products. In this work, the enantioseparation of MBC has been performed by EKC and applied to the analysis of the MBC enantiomers in cosmetic creams. Different experimental conditions (type and concentration of the chiral selector, temperature, and sample solvent) have been optimized. Due to the neutral nature of this compound, anionic CD derivatives were investigated as chiral selectors. Carboxymethylated-b-CD (CM-b-CD) showed the highest chiral separation power, observing that a 15 mM concentration of this CD at a working temperature of 157C enabled to obtain the highest enantioresolution. However, under these conditions, tailing of peaks obtained for the enantiomers was observed. The addition of increasing concentrations of the neutral a-CD to CM-b-CD at a 15 mM concentration in a 100 mM borate buffer at pH 9.0 improved the enantiomeric separation and decreased peak tailing. The use of DMF for the total dissolution of the cosmetic creams, and methanol:water (1:1 v/v) for appropriate dilution enabled to observe good shape and size for the peaks of the MBC enantiomers. After optimizing a method for the preconditioning of the capillary, the analytical characteristics of the chiral separation method for the analysis of MBC were investigated. Linearity, LODs and LOQs, precision (instrumental repeatability, method repeatability, intermediate precision), accuracy, and selectivity were evaluated.The method was applied to analyze MBC enantiomers contained in two commercial cosmetic creams containing racemic MBC and to study the skin absorption of this compound with time.

show abstract

Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives

Cited by 33 publications

References 27 publications

Enantiomeric separation of organophosphorus pesticides by capillary electrophoresis

Enantiomeric separation of organophosphorus pesticides by capillary electrophoresis

Chiral analysis of pollutants and their metabolites by capillary electromigration methods

Enantioselective separation of the sunscreen agent 3-(4-methylbenzylidene)-camphor by electrokinetic chromatography: Quantitative analysis in cosmetic formulations

Contact Info

Product

Resources

About