1979
DOI: 10.1111/j.1365-2621.1979.tb03485.x
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Reactive Lysine in Foodstuffs as Measured by a Rapid Dye‐binding Procedure

Abstract: PBSTRACTThe dye-binding capacity (DBC) of basic groups in the proteins of test materials for Acid Orange 12 is measured before and after reaction with propionic anhydride to block the lysine groups. The difference in moles dye bound as a result of the reaction agrees well with the lysine moles present, as determined with fluoridinitrobenzene and by ion-exchange chromatography, for over 50 samplesfish, meat, milk, barley, peanut, grain legumes etc. The procedure takes approximately 40 min using commercial dye-b… Show more

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Cited by 116 publications
(50 citation statements)
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“…Por otra parte, la LPM, a diferencia de la LP, tiene mayor contenido de vitaminas, algunas de las cuales son mas inestables en las condiciones de elaboration y almacenamiento: se eligio medir el acido ascorbico por considerarse la vitamina mas labil bajo las condiciones de almacenamiento establecidas 1 *. Se determine mdice de peroxides 1 ' cada 15 dfas, lisina disponible 8 , acido ascorbico 11 y acido linoleico* mensualmente durante su almacenamiento a 20°C y 37°C por un perfodo de un ano.…”
Section: Materials Y Mltodosunclassified
“…Por otra parte, la LPM, a diferencia de la LP, tiene mayor contenido de vitaminas, algunas de las cuales son mas inestables en las condiciones de elaboration y almacenamiento: se eligio medir el acido ascorbico por considerarse la vitamina mas labil bajo las condiciones de almacenamiento establecidas 1 *. Se determine mdice de peroxides 1 ' cada 15 dfas, lisina disponible 8 , acido ascorbico 11 y acido linoleico* mensualmente durante su almacenamiento a 20°C y 37°C por un perfodo de un ano.…”
Section: Materials Y Mltodosunclassified
“…Nitrogen solubility was carried according to Lyman et al procedure (5). Available lysine was determined by (7). Free and total gossypol determinations was carried according to the method of Pons et al (8).…”
Section: Methods Of Analysismentioning
confidence: 99%
“…Color of biological products is drastically influenced during the thermal processing mainly due to the non-enzymatic browning reaction (Maillard) which leads to protein deterioration. In extrusion processing, high temperature and semi-dry condition favors the browning reaction which results in amino acid destruction and unavailability of some of essential amino acids such as lysine and tryptophan (Hurrell et al, 1979). The required reduced sugar for this process is provided by hydrolysis and dextrinization of starch component of the feed blend induced by mechanical shearing (Shagun & Harper, 1980).…”
Section: Pellet Durability Index (Pdi)mentioning
confidence: 99%