Reactive oxygen species: the unavoidable environmental insult?

Gracy, Robert W.; Talent, John M.; Kong, Yongli; Conrad, Craig C.

doi:10.1016/s1383-5742(99)00027-7

Cited by 151 publications

(77 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The animals were kept in specially designed well-ventilated cages, their movements were restricted and no anaesthesia was administered. The animals were exposed to whole body radiation at a rate of 1.4 Gy/min in a field size of about 25ϫ25 cm 2 and at a distance of 70 cm from the source.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Kolaviron, a Biflavonoid from Garcinia kola Seeds, in Brain of Wistar Albino Rats Exposed to Gamma-Radiation

Adaramoye

2010

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

This study was designed to evaluate the protective effect of kolaviron (KV), a biflavonoid from Garcinia kola seeds, against g g-radiation (5 Gy)-induced oxidative stress in brain of Wistar rats. Vitamin C (VC) served as standard antioxidant. Forty-four rats were divided into 4 groups of 11 animals each. One group was un-irradiated (normal), two groups were treated with KV and VC (250 mg/kg) for 6 weeks prior to and 8 weeks after irradiation, and fourth group was only irradiated. Rats were sacrificed 1 and 8 weeks after irradiation. Cellular alterations were monitored using changes in the levels of malondialdehyde (MDA)-an index of lipid peroxidation, superoxide dismutase (SOD), glutathione-S-transferase (GST), reduced glutathione (GSH), catalase (CAT), alanine and aspartate aminotransferases (ALT and AST), urea and creatinine. MDA levels increased significantly (pϽ Ͻ0.05) by 90% and 151% after 1 and 8 weeks of irradiation. Furthermore, levels of GSH and antioxidant enzymes were significantly (pϽ Ͻ0.05) decreased in g g-irradiated animals. GSH and GST decreased by 61% and 43% after 1 week, and by 75% and 74%, after 8 weeks of exposure, respectively. g g-Irradiation decreased SOD and CAT levels by 53% and 68%, respectively, and caused significant ( pϽ Ͻ0.05) increases in serum ALT, AST and urea after 8 weeks of exposure. Treatment with KV and VC significantly decreased the levels of MDA, ALT, AST and urea. The antioxidant indices were significantly ameliorated in KV-treated animals. These data suggest that kolaviron may protect against g g-radiation-induced oxidative stress in brain of exposed rats.

show abstract

Section: Methodsmentioning

confidence: 99%

Protective Effect of Kolaviron, a Biflavonoid from Garcinia kola Seeds, in Brain of Wistar Albino Rats Exposed to Gamma-Radiation

Adaramoye

2010

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Oxidative damage to DNA can result in a variety of lesions, including base damage, inter-and intrastrand crosslinks, DNA-protein crosslinks, single-strand breaks and doublestrand breaks (2,3). Fixation of such damage has been im-plemented in numerous diseases such as diabetes, heart failure, neurodegeneration, aging and cancer [for review, see ref.…”

Section: Introductionmentioning

confidence: 99%

Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H₂O₂

Hachiya¹,

Akashi²

2005

Radiation Research

View full text Add to dashboard Cite

“…Such modifications may lead to mutation, carcinogenesis, cellular aging and death (18). A recent study (19) suggested that the brain in AD and aged people may be subject to the insult of oxidative stress, and an increase in levels of brain DNA oxidation exists in both aging and AD.…”

Section: +mentioning

confidence: 99%

Antioxidative Activity of Natural Isorhapontigenin

Wang

Lin

Liu

2001

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

ABSTRACT-Isorhapontigenin (ISOR), isolated from Belamcanda chinensis, is a derivative of stilbene. Its chemical structure is very similar to that of resveratrol, with a potent antioxidative effect. In the present study, we investigated the antioxidative activity of ISOR in vitro. Oxidative damage of rat liver microsomes, brain mitochondria and synaptosomes was induced by Fe 2+ -Cys, VitC-ADP-Fe 2+ and H2O2, respectively. The formation of malondialdehyde (MDA), decrease of reduced glutathione (GSH) and increase of ultra-weak chemiluminescence during the lipid peroxidation process were determined. In addition, the characteristic ultra-weak chemiluminescence of oxidative DNA damage induced by CuSO 4-Phen-VitC-H2O2 system was studied. The results showed that ISOR significantly inhibited MDA formation in liver microsomes, brain mitochondria and synaptosomes induced by Fe 2+-Cys. Also, ISOR markedly prevented the decrease of GSH in mitochondria and synaptosomes induced by H2O2 and the increase of ultra-weak chemiluminescence during lipid peroxidation induced by VitC-ADP-Fe 2+ as well as oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2. The effects of ISOR at 10 -5 and 10 -6 mol/ L on the MDA formation and decrease of GSH were similar to that of the classical antioxidant vitamin E (10 -4 mol/ L). It may be concluded that ISOR possessed potent antioxidative activity and was much more potent than vitamin E.

show abstract

Reactive oxygen species: the unavoidable environmental insult?

Cited by 151 publications

References 7 publications

Protective Effect of Kolaviron, a Biflavonoid from Garcinia kola Seeds, in Brain of Wistar Albino Rats Exposed to Gamma-Radiation

Protective Effect of Kolaviron, a Biflavonoid from Garcinia kola Seeds, in Brain of Wistar Albino Rats Exposed to Gamma-Radiation

Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H₂O₂

Antioxidative Activity of Natural Isorhapontigenin

Contact Info

Product

Resources

About

Reactive oxygen species: the unavoidable environmental insult?

Cited by 151 publications

References 7 publications

Protective Effect of Kolaviron, a Biflavonoid from Garcinia kola Seeds, in Brain of Wistar Albino Rats Exposed to Gamma-Radiation

Protective Effect of Kolaviron, a Biflavonoid from Garcinia kola Seeds, in Brain of Wistar Albino Rats Exposed to Gamma-Radiation

Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H2O2

Antioxidative Activity of Natural Isorhapontigenin

Contact Info

Product

Resources

About

Catalase Regulates Cell Growth in HL60 Human Promyelocytic Cells: Evidence for Growth Regulation by H₂O₂