Recent advances in polymeric microspheres for parenteral drug delivery—part 2

Mao, Shirui; Guo, Chunqiang; Shi, Yi; Li, Luk Chiu

doi:10.1517/17425247.2012.717926

Cited by 24 publications

(10 citation statements)

References 98 publications

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“…28,29 PLGA is a biocompatible and biodegradable polymer, and the parenteral products using this polymer have been already approved by the US Food and Drug Administration for human use. 30 Eudragit RL, which contains positively charged quaternary ammonium groups, was used in combination with PLGA to electrostatically interact with the anionic HA, forming an ionically crosslinked network. Aksungur et al 31 reported that there was no difference in the cytotoxicity of PLGA/Eudragit RL NPs and PLGA NPs after 24 or 48 hours of cell incubation.…”

Section: Results and Discussion Preparation And Physicochemical Charamentioning

confidence: 99%

“…The concentration of HA in aqueous medium was set to 2.0 mg/mL because the concentration of HA in synovial fluid was reduced from 3.5 to below 2.2 mg/mL in osteoarthritic conditions. 30 Figure 4A displays representative optical micrographs of the filamentous NP/HA aggregates. Immediately upon mixing DiR-loaded NPs with HA under gentle agitation, discontinuous and filamentous aggregates ranging in diameter from 5 to 50 μm were precipitated without additional treatment ( Figure 3A).…”

Section: Hyperspectral Imaging Of Np/ha Aggregatesmentioning

confidence: 99%

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Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint

Kim¹,

Ho²,

Lee³

et al. 2015

IJN

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Positively surface-charged poly(lactide- co -glycolide) (PLGA)/Eudragit RL nanoparticles (NPs) were designed to increase retention time and sustain release profile in joints after intra-articular injection, by forming micrometer-sized electrostatic aggregates with hyaluronic acid, an endogenous anionic polysaccharide found in high amounts in synovial fluid. The cationic NPs consisting of PLGA, Eudragit RL, and polyvinyl alcohol were fabricated by solvent evaporation technique. The NPs were 170.1 nm in size, with a zeta potential of 21.3 mV in phosphate-buffered saline. Hyperspectral imaging (CytoViva ® ) revealed the formation of the micrometer-sized filamentous aggregates upon admixing, due to electrostatic interaction between NPs and the polysaccharides. NPs loaded with a fluorescent probe (1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide, DiR) displayed a significantly improved retention time in the knee joint, with over 50% preservation of the fluorescent signal 28 days after injection. When DiR solution was injected intra-articularly, the fluorescence levels rapidly decreased to 30% of the initial concentration within 3 days in mice. From these findings, we suggest that PLGA-based cationic NPs could be a promising tool for prolonged delivery of therapeutic agents in joints selectively.

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Section: Results and Discussion Preparation And Physicochemical Charamentioning

confidence: 99%

Section: Hyperspectral Imaging Of Np/ha Aggregatesmentioning

confidence: 99%

Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint

Kim¹,

Ho²,

Lee³

et al. 2015

IJN

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“…PHB microspheres are useful for targeting drugs to specific infection sites and for prolonged drug release (Mao ., 2012;Joshi and Patel, 2012). The product of degradation of PHB is 3-hydroxy butyric acid which is normally present in human blood and this is advantageous for PHB to be used in medical field (Siraj ., 2014).…”

Section: Microsphere Preparationmentioning

confidence: 99%

Preparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization

Swornakumari¹,

Meignanalakshmi²,

Legadevi³

et al. 2018

JEB

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Aim :Methodology : Results :Interpretation :Biodegradable poly-3-hydroxybutyrate is one of the most common biopolymer which is used in different fields such as medicine, agriculture, textile, industrial and food packaging. PHB microspheres are useful for targeting drugs to specific infection sites and for prolonged drug release. The present study focus on microsphere preparation for effective controlled drug release using poly-3-hydroxy butyrate biopolymer.In the present study, poly-3-hydroxy butyrate microspheres were prepared using solvent evaporation technique and characterized by SEM and FTIR. Poly-3-hydroxy butyrate microspheres were encapsulated with BSA and gentamicin. Drug encapsulation efficiency was determined. drug release profile and cytotoxicity was also studied.The prepared microspheres were of 2μm in size. Microspheres fabricated with 1% polyvinylalcohol showed encapsulation efficiency of 94.3% and 90.27% with BSA and gentamicin, respectively. The release studies in simulated body fluid, phosphate buffered saline and contact lens solution showed initial burst release followed by controlled release. cytotoxicity analysis showed 98% and 95% viability of cells in 3T3L1 cell line for microsphere encapsulated with BSA and gentamicin, respectively.Poly-3-hydroxy butyrate microspheres were found to release BSA and gentamicin in a controlled manner and were found to be non-toxic by cytotoxicity studies. In vitro in vitro in vitro In vitro in vitroJournal Home page : www.jeb.co.in « E-mail : editor@jeb.co.in Journal of Environmental BiologyPreparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization Surface morphology of PHB microspheres was analyzed using scanning electron microscope (HITACHI model S-3000H).PHB polymer and PHB microspheres were subjected to Fourier Transform Infrared Spectroscopy analysis by FTIR spectrophotometer. Spectra were documented in 4000 cm to 400 cm range Two mg of microspheres were treated with 2 ml of 0.5 M sodium hydroxide for 15 min at 60ºC. To the mixture, 900 of 1 N hydrochloric acid was added to neutralize the samples and the volume was adjusted to 5 ml with distilled water. To the mixture, 3 ml of 0.65M boric acid, 0.5 ml iodine solution and 1.5 ml of distilled water were added. After 15 min incubation, absorbance was measured at 690 nm.Microsphere preparation (1% PVA at 800 rpm) procedure was followed with addition of BSA and gentamicin along with PHB (Shishatskaya ., 2008) Five mg of BSA and gentamicin loaded microspheres were immersed separately in 3 ml of contact lens solution, phosphate buffer (PBS) (pH, 7.2) and simulated body fluid (SBF) (Kokubo ., 1990). The Composition of simulated body fluid consisted pure water, sodium chloride, sodium bicarbonate, potassium chloride, potassium phosphate dibasic trihydrate, magnesium chloride hexahydrate, calcium chloride, Sodium sulphate and Tris base. They were kept in a shaker at 250 rpm at 37ºC. About 1ml of the sample was collected at regular intervals, till 24hrs. Each sample was ...

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“…Under this condition, the production yield is relatively high at about 75–80% for the formulation of polymer: drug ratio did not affect the yield. The mean particle size of the prepared microspheres was found to be 25.3 ± 5.4 μ m and was suitable for implant delivery (prefer 5–30 μ m) . The drug loading of the formulation was 18.5 ± 2.3%.…”

Section: Resultsmentioning

confidence: 96%

Novel Alginate–Chitosan Composite Microspheres for Implant Delivery of Vancomycin and In Vivo Evaluation

Mao

Zhao

et al. 2016

Chem Biol Drug Des

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In this study, vancomycin loaded alginate-chitosan composite microspheres were developed by emulsion cross-linking method. The in vitro and vivo characterizations were done to evaluate the feasibility of application. Our experimental results showed that the emulsification cross-linking technique appeared to be a feasible method for the preparation of alginate-chitosan composite microspheres. The microspheres were spherical in shape and the mean particle size and drug loading were 25.3 ± 5.4 μm and 18.5 ± 2.3% respectively. A sustained vancomycin release was realized i.e. the amount of cumulative release increased in a time frame of 24 h to reach an amount i.e. ~68%. The model that fit best for vancomycin released from the microspheres was the Higuchi kinetic model with a correlation coefficient r = 0.9996. In vivo results showed that the application of microspheres not only reduced the toxicity, but also maintained effective drug concentration. In addition, no severe signs of epithelial necrosis and sloughing of epithelial cells were detected in histological studies.

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Recent advances in polymeric microspheres for parenteral drug delivery—part 2

Cited by 24 publications

References 98 publications

Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint

Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint

Preparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization

Novel Alginate–Chitosan Composite Microspheres for Implant Delivery of Vancomycin and In Vivo Evaluation

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