Recent developments in CE‐based detection methods for food‐borne pathogens

Shin, Gi Won; Hwang, Hee Sung; Chung, Boram; Jung, Gyoo Yeol

doi:10.1002/elps.200900682

Cited by 21 publications

(19 citation statements)

References 143 publications

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“…Other techniques as gas chromatography (GC) still have an important role in food analysis, for instance, for the analysis of volatile fractions or fatty acids in foods [20]. Electrodriven separation techniques such as capillary electrophoresis (CE) or microchip capillary electrophoresis have found important applications in food analysis as can be deduced from the many review works devoted to this topic [21,22], including the detection of genetically modified organisms [23,24], nucleosides and nucleotides in foods [25], analysis of contaminants in emerging food safety issues and food traceability [26], and food-borne pathogens [27].…”

Section: Food Analysis: Current State Of the Art Methodologies And mentioning

confidence: 99%

Food Analysis: Present, Future, and Foodomics

Cifuentes

2012

ISRN Analytical Chemistry

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This paper presents a revision on the instrumental analytical techniques and methods used in food analysis together with their main applications in food science research. The present paper includes a brief historical perspective on food analysis, together with a deep revision on the current state of the art of modern analytical instruments, methodologies, and applications in food analysis with a special emphasis on the works published on this topic in the last three years (2009)(2010)(2011). The article also discusses the present and future challenges in food analysis, the application of "omics" in food analysis (including epigenomics, genomics, transcriptomics, proteomics, and metabolomics), and provides an overview on the new discipline of Foodomics.

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Section: Food Analysis: Current State Of the Art Methodologies And mentioning

confidence: 99%

Food Analysis: Present, Future, and Foodomics

Cifuentes

2012

ISRN Analytical Chemistry

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“…To determine the prevalence of SFG rickettsiae in Amblyomma variegatum from seven districts across Uganda, Nakao et al [124] obtained hypervariable IGSs (dksA-xerC, mppA-purC, and Figure 18: Scheme of the complete multistep process of MNPs isolation and analysis on a single CE chip [118]. The chip priming (1), addition of the MNPs into the wells (2), flushing of the particles with phosphate buffer (3), immobilization (4), removal of unbound compounds (5), flushing of the particles with phosphate buffer (6), elution (heating to 85 °C for 5 min) (7), addition of 5% loading buffer (8), addition of gel with fluorescent stain and ladder (9), CE analysis (10).…”

Section: Detecting Mycoplasma Chlamydia and Rickettsiamentioning

confidence: 99%

“…It can be used on a microscale and combined with various nucleic acid detection strategies to avoid many types of false positive and negative results [5], making CE an alternative and promising diagnostic tool for rapid diagnosis of infectious diseases [7,8]. Thus, this review focuses on CE based on nucleic acid detection in the application of human infectious disease diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

Lian

Zhao

2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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Rapid transmission, high morbidity, and mortality are the features of human infectious diseases caused by microorganisms, such as bacteria, fungi, and viruses. These diseases may lead within a short period of time to great personal and property losses, especially in regions where sanitation is poor. Thus, rapid diagnoses are vital for the prevention and therapeutic intervention of human infectious diseases. Several conventional methods are often used to diagnose infectious diseases, e.g. methods based on cultures or morphology, or biochemical tests based on metabonomics. Although traditional methods are considered gold standards and are used most frequently, they are laborious, time consuming, and tedious and cannot meet the demand for rapid diagnoses. Disease diagnosis using capillary electrophoresis methods has the advantages of high efficiency, high throughput, and high speed, and coupled with the different nucleic acid detection strategies overcomes the drawbacks of traditional identification methods, precluding many types of false positive and negative results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic detection to the diagnosis of human infectious diseases, and offers an introduction to the limitations, advantages, and future developments of this approach.

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“…In such cases, capillary electrophoresis can be used as a sensitive method to quantitatively analyze and compare the presence of each target pathogen gene (Barakat et al, 2014;Chung et al, 2012;Shin et al, 2014;Shin et al, 2010). Capillary electrophoresis can be successfully applied in cases of insect cross infection with several pathogens to quantitatively evaluate relative importance of infectious agents based on the ratio of target gene expression (Thomas et al, 2010b);…”

Section: Pathogen Detection In Diseased Insects Using Multiplex Pcrmentioning

confidence: 99%

Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis

Kwak¹,

Nam²,

Choi³

et al. 2015

International Journal of Industrial Entomology

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Beetles Protaetia brevitarsis seulensis Kolbe (Coleoptera: Cetoniidae) and Allomyrina dichotoma Linn. (Coleoptera: Scarabaeidae) are widely used in traditional medicine, and the number of insect-rearing farms is increasing in South Korea. The purpose of this study was to establish a multiplex PCR-based assay for rapid simultaneous detection of multiple pathogens causing insect diseases. Six insect parasites such as fungi Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae), bacteria Bacillus thuringiensis Berliner (Bacillales: Bacillaceae), Pseudomonas aeruginosa Migula (Pseudomonadales: Pseudomonadaceae), and Serratia marcescens Bizio (Enterobacteriales: Enterobacteriaceae), and Oryctes rhinoceros nudivirus were chosen based on the severity and incidence rate of insect diseases in South Korea. Pathogen-specific primers were designed and successfully applied for simultaneous detection of multiple infectious agents in farm-bred insects P. b. seulensis and A. dichotoma using multiplex PCR and high resolution capillary electrophoresis. Our results indicate that multiplex PCR is an effective and time-saving method for simultaneous detection of multiple infections in insects, and the QIAxcel capillary electrophoresis system is useful for quantitative evaluation of the individual impact of each infectious agent on the severity of insect disease. The approach designed in this study can be utilized for rapid and accurate diagnostics of infection in insect farms.© 2015 The Korean Society of Sericultural Sciences Int. J. Indust. Entomol. 30(2), 64-74 (2015) IntroductionFollowing a global trend of using natural products from insects, the Korean government has been encouraging the development of insect resources for environmental and dietary purposes, traditional medicine, and as pets (Kim et al., 2013 Vol. 30, No. (1), pp. 64-74 (2015) Pseudomonas aeruginosa is reported as a virulent bacterial pathogen not only for insects but also for mammals (Jander et al., 2000), where it causes infection of the respiratory tract, especially in cystic fibrosis patients (Spiker et al., 2004). Similar to Pseudomonas aeruginosa, Serratia marcescens is known as an opportunistic bacterium (El-Aasar et al., 2013); it is also used to control apple maggot fly population and as a source of insecticidal agents such as toxin luxA (Hejazi and Falkiner, 1997; Lauzon et al., 2003). Oryctes rhinoceros virus Rhabdionvirus oryctes Hüger was first discovered in oil palms in Malaysia 40 years ago and is currently used for the control of rhinoceros beetle population in Malaysia (Huger, 2005;Ramle et al., 2005).We have identified this virus in an A. dichotoma-breeding farm in 2014 in Korea (Lee, 2015).Extensive large-scale insect breeding has resulted in frequent occurrence of insect infection due to multiple pathogens. Here, we developed a multiplex PCR-based approach that allowed simultaneous detection of six insect pathogens using specific primer se...

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Recent developments in CE‐based detection methods for food‐borne pathogens

Cited by 21 publications

References 143 publications

Food Analysis: Present, Future, and Foodomics

Food Analysis: Present, Future, and Foodomics

Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis

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