Recyclable Decoration of Amine-Functionalized Magnetic Nanoparticles with Ni²⁺ for Determination of Histidine by Photochemical Vapor Generation Atomic Spectrometry

Abstract

It is critically important to accurately determine histidine since it is an indicator for many diseases when at an abnormal level. Here, an inexpensive and simple method using an amine-functionalized magnetic nanoparticle-based Ni(2+)-histidine affinity pair system was developed for highly sensitive and selective detection of histidine in human urine by photochemical vapor generation atomic spectrometry. Ni(2+) was first bound to the amine groups of the amine-functionalized magnetic nanoparticles and then liberated to solution via the highly specific interaction between the histidine and Ni(2+) in the presence of histidine. The liberated histidine-Ni(2+) complex was exposed to UV irradiation in the presence of formic acid to form gaseous nickel tetracarbonyl, which was separated from the sample matrix and determined by atomic absorption/fluorescence spectrometry. Compared to other methods, this approach promises high sensitivity, simplicity in design, and convenient operation. The need for organic solvents, enzymatic reactions, separation processes, chemical modification, expensive instrumentations, and sophisticated and complicated pretreatment is minimized with this strategy. A limit of detection of 1 nM was obtained and provided tens-to-hundreds of fold improvements over that achieved with conventional methods. The protocol was evaluated by analysis of several urine samples with good recoveries and showed great potential for practical application.

Quartz crystal microbalance based histidine sensor

“…Histidine, an essential bioactive molecule, is highly important for human growth and repair of tissues and behaves as a neurotransmitter in human muscular and the central nervous system [1][2][3][4][5]. Additionally, histidine regulates metal ions transmission in biological systems and constitutes the active site of many enzymes and natural proteins [6,7].…”

Synthesis of hierarchical nickel anchored on Fe3O4@SiO2and its successful utilization to remove the abundant proteins (BHb) in bovine blood

“…75 As an important magnetic material, nickel-based nanomaterials have been successfully synthesized by using a variety of approaches. [28][29] The nickel metal was not only used for magnetic support, but also can enrich the histine protein with the specific metal affinity to polyhistidine groups. Mirkin and 80 co-workers demonstrated the efficient and selective separation of His-tagged proteins using Ni containing magnetic nanorods with a diameter of about 300 nm.…”

Recent advances in biological detection with magnetic nanoparticles as a useful tool

“…An inexpensive and simple method using an aminefunctionalized MNP-based Ni 2+ -histidine affinity-pair system for the highly sensitive and selective detection of histidine in human urine by photochemical vapor generation atomic spectrometry was developed by Hu et al [54]. This convenient method achieved a high sensitivity of 1 nmol/L (tens-to-hundreds times better than sensitivity achieved with conventional methods) and shows great potential for practical application.…”