Reduced ischemia-reperfusion injury in muscle Experiments in rats with EPC-K1, a new radical scavenger

Hirose, Jun; Yamaga, Makio; Ide, Junji; Tanoue, Masahiro; Takagi, Katsumasa

doi:10.3109/17453679708996179

Cited by 18 publications

(6 citation statements)

References 12 publications

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“…Indeed, some studies report that administration of antioxidants and free-radical scavengers at the onset of reperfusion had a protective effect on the I-R injured muscles. [5][6][7] The present study also demonstrates, for the first time, the ability of LLLT to induce synthesis and expression of hsp-70i in I-R injured skeletal muscles. We demonstrated a similar phenomenon recently in the ischemic heart.…”

Section: Discussionmentioning

confidence: 94%

Protection of Skeletal Muscles from Ischemic Injury: Low-Level Laser Therapy Increases Antioxidant Activity

Avni

Levkovitz

Maltz

et al. 2005

Photomedicine and Laser Surgery

105

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The present study describes for the first time the ability of LLLT to significantly prevent degeneration following ischemia/reperfusion injury in skeletal muscles, probably by induction of synthesis of antioxidants and other cytoprotective proteins, such as hsp-70i. The elevation of antioxidants was also evident in intact muscle following LLLT. The above phenomenon may also be of clinical relevance in scheduled surgery or microsurgery requiring extended tourniquet applications to skeletal muscle followed by reperfusion.

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Section: Discussionmentioning

confidence: 94%

Protection of Skeletal Muscles from Ischemic Injury: Low-Level Laser Therapy Increases Antioxidant Activity

Avni

Levkovitz

Maltz

et al. 2005

Photomedicine and Laser Surgery

105

View full text Add to dashboard Cite

show abstract

“…in muscle (Hirose et al, 1997), lung (Nagahiro et al, 1997), liver (Yagi et al, 1997(Yagi et al, , 1992, brain (Kato et al, 2003), and during myocardial infarction (Kuribayashi et al, 1996;Yamada et al, 1998a). NOinduced neurotoxicity is reduced by EPC-K1 by preventing apoptosis and mitochondrial dysfunction in cerebellar granule cells (Wei et al, 1999a).…”

Section: Molecular and Cellular Effects Of A-tocopheryl Phosphate Andmentioning

confidence: 96%

Cellular, molecular and clinical aspects of vitamin E on atherosclerosis prevention

Munteanu¹,

Zingg

2007

Molecular Aspects of Medicine

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“…α‐Tocopherol (vitamin E), an antioxidant in biological membranes, has also been reported to exert a protective effect against oxidative cell damage and to increase ATP resynthesis, as I/R injury is also characterized by the loss of high energy phosphates (22). Protective effects on I/R injury in skeletal muscle have been shown after supplementation with vitamin E in combination with vitamin C i. v. (8, 9, 10, 23) and in combination with allopurinol by intraperitoneal application (1), which however is not practicable in patient treatment. Positive influence of oral intake of vitamin E was documented in patients undergoing aortic surgery (2, 21).…”

Section: Introductionmentioning

confidence: 99%

alpha-Tocopherol Pretreatment Reduces Ischaemia/Reperfusion Injury in Rabbit Skeletal Muscle

et al. 2002

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Summary: Background: Ischaemia/reperfusion (I/R) injury is characterized by the production of oxygen free radicals and the loss of high energy phosphates. α‐Tocopherol (vitamin E) has been reported to play a protective role against free radical damage. Therefore, its i. v. supplementation prior to onset of ischaemia was investigated in an established I/R injury model. Methods: New Zealand white rabbits were subjected to a bilateral hind limb ischaemia/reperfusion of 2.5 h/2 h without any treatment (IR group), whereas in the VE group the vitamin E emulsion Tocovenös® was given i. v. for 10 min in a dose of 3 mg/kg bw. A SHAM group served as a control. Intramuscular phosphocreatine (PCr) and adenosine triphosphate (ATP), muscle interfibre area (MIFA), and microvessel cross‐sectional area (MVCSA) were determined and electron microscopy was performed. Results: In the IR group, PCr decreased by 65 % of the initial level after 2.5 h of ischaemia and a further depletion was noticed after 2 h of reperfusion (20.9 ± 1.1 vs 6.3 ± 3.0 µmol/g wet wt, P < 0.01). By contrast, ATP remained stable after ischaemia but dropped dramatically after reperfusion (6.5 ± 0.2 vs 2.1 ± 8.0 µmol/g wet wt, P < 0.05). In the VE group, PCr and ATP did not differ significantly from the SHAM group at the end of reperfusion. Consequently, ATP in this group was significantly higher as compared with the IR group (4.9 ± 0.9 vs 2.1 ± 8.0 µmol/g wet wt, P < 0.05). In the IR group, prominent interstitial oedema formation was found after reperfusion expressed by the increase in MIFA (28.2 ± 4.2 vs 13.7 ± 1.6 %, P < 0.05). In the VE group, only a moderate interstitial oedema was found (18.7 ± 3.4 %). Severe microvessel constriction was observed after reperfusion in both experimental groups. Electron microscopy revealed fewer tissue changes in the VE group as compared with the IR group. Conclusions: α‐Tocopherol pretreatment had a protective effect on I/R injury in skeletal muscle expressed by recovery of PCr after reperfusion and by prevention of ATP depletion. Although microvascular constriction could not be prevented, antioxidative treatment reduced interstitial oedema and preserved muscle morphology.

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Reduced ischemia-reperfusion injury in muscle Experiments in rats with EPC-K1, a new radical scavenger

Cited by 18 publications

References 12 publications

Protection of Skeletal Muscles from Ischemic Injury: Low-Level Laser Therapy Increases Antioxidant Activity

Protection of Skeletal Muscles from Ischemic Injury: Low-Level Laser Therapy Increases Antioxidant Activity

Cellular, molecular and clinical aspects of vitamin E on atherosclerosis prevention

alpha-Tocopherol Pretreatment Reduces Ischaemia/Reperfusion Injury in Rabbit Skeletal Muscle

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