Reduction of Escherichia Coli Using Metal Plates with the Influenced of Applied Low Current and Physical Barrier of Filter Layers

Versoza, Michael; Jung, Wonseok; Barabad, Mona Loraine M.; Ko, Sangwon; Kim, Minjeong; Park, Duckshin

doi:10.3390/ijerph16203887

Cited by 5 publications

(9 citation statements)

References 31 publications

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“…Some studies suggest that electrical current alone does not have a bactericidal effect; however, bacteria in biofilms can synergistically be controlled when applying an electrical current together with biocides or antimicrobial agents (Costerton et al 19941996; Versoza et al 2019…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of copper ions against Mycobacterium avium subsp. paratuberculosis and bacterial communities in naturally contaminated raw cow’s milk

Steuer

Tejeda²,

Martínez

et al. 2020

J. Appl. Microbiol.

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Aim The focus of the present study was to evaluate the copper ions treatment on the viability of Mycobacterium avium subsp. paratuberculosis (MAP) and other bacterial communities in cow’s milk. Methods and Results A copper ions treatment was evaluated in naturally contaminated cow’s milk to assay MAP load and/or viability, and relative abundance of other bacterial communities. In addition, physical–chemical analyses of the milk were also performed. All analyses were carried out before and after a copper ions treatment. After copper ions treatment, pH and copper concentration markedly increased in milk; the numbers of viable MAP significantly decreased. The relative abundance of the four target phyla decreased, with the phyla Bacteroidetes and Firmicutes surviving treatment in higher proportions (4 and 2·1% of original populations, respectively). A progressively higher percentage of dead bacterial cells after 5 and 20 min copper ions treatments was found (12 and 35%, respectively). Conclusion With the exception of some MAP‐tolerant strains, we have once again demonstrated that copper ions have a significant inactivating effect on MAP as well as certain other bacterial communities found in naturally contaminated cow’s milk. Significance and Impact of the Study This study showed a significant inactivation of both MAP and other bacteria by copper ions in raw cow’s milk, information that could be useful as a tool for MAP control.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of copper ions against Mycobacterium avium subsp. paratuberculosis and bacterial communities in naturally contaminated raw cow’s milk

Steuer

Tejeda²,

Martínez

et al. 2020

J. Appl. Microbiol.

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“…Conversely, the stimulation of copper plates with an electric current caused a greater release of copper ions than that without an electric current. A recent study determined that electrically stimulated copper plates led to double the reduction in bacteria (E. coli), compared with electrically stimulated stainless-steel plates [22], which could be related to the production of free radicals such as hydroxyl ions and the production of reactive oxygen species (ROS) [37], favoring the formation of cuprous oxide and cupric oxide [38].…”

Section: Discussionmentioning

confidence: 99%

“…Our experiment used a glass receptacle copper device in which copper plates of high purity (99%) were immersed in a liquid matrix and were stimulated with electricity [14,15]. This method allows a greater release of ions than would be the case without electricity, as shown in a study, in which copper plates stimulated with electricity resulted in reduced growth of E. coli bacteria by 79.5 ± 2.34% [22]. Interestingly, some MAP strains managed to tolerate this novel copper treatment [14,15].…”

Section: Introductionmentioning

confidence: 99%

More Insights about the Efficacy of Copper Ion Treatment on Mycobacterium avium subsp. paratuberculosis (MAP): A Clue for the Observed Tolerance

et al. 2022

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Background: Scientific evidence is scarce for the antimicrobial effect of copper on bacteria characterized as more resistant. Using Mycobacterium avium subsp. paratuberculosis (MAP), a highly resistant microorganism, as a pathogen model, copper ion treatment has shown a significant bactericidal effect; however, the sustainability of MAP against copper toxicity was also reported in several studies. Accordingly, the present study aimed to evaluate the impacts of copper on MAP. Methodology: This study considered physicochemical properties and copper concentration in a buffer since it could modulate MAP response during the application of copper treatment. Results: Despite the efficacy of copper ions in significantly reducing the MAP load in Phosphate Buffered Saline, some MAP cells were able to survive. The copper concentration generated by the copper ion treatment device increased significantly with increasing exposure times. MAP bacterial load decreased significantly when treated with copper ions as the exposure times increased. An increase in pH decreased oxygen consumption, and an increase in conductivity was reported after treatment application. Conclusions: Even with higher concentrations of copper, the efficacy of MAP control was not complete. The concentration of copper must be a key element in achieving control of highly resistant microorganisms.

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“…The virus used for this experiment was the MS2 bacteriophage ( Escherichia coli bacteriophage MS2, American Type Culture Collection [ATCC] 15597-B1; ATCC, Manassas, VA, USA). Unlike the viruses that are harmful to the human body, this virus is non-pathogenic and has been used in several studies [ 5 , 8 , 26 , 27 , 28 , 29 ]. The virus was prepared by mixing a stock solution (100 μL) in 50 mL of sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…This study was conducted entirely at room temperature (25 °C) and at a relative humidity of 50% or less; the sampling was performed three times. The experimental preparation and inactivation efficiency calculations in this study were based on previous studies by Versoza et al [ 5 , 8 , 29 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Brush-Type Ionizer Materials on Virus Inactivation

Heo

Lee

Park

2022

Toxics

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Many studies have found that bioaerosols are harmful to humans. In particular, infectious viruses, such as the virus that causes COVID-19, are increasing. Therefore, the research on methods for reducing bioaerosols is becoming progressively more important. The purpose of this study was to improve the existing electrostatic precipitator, which generates high concentrations of ozone, by reducing bioaerosols effectively without significant ozone production. A brush-type ionizer was studied as a replacement for the existing electrostatic precipitator. The study, which was conducted at the laboratory scale, determined the amounts of ions generated with different ionizer materials (carbon, copper, and stainless steel) and voltages (−1, −2, and −3 kV), as well as it compared the virus inactivation efficiency under the various conditions. As a result, about two million ions were produced when a voltage of −3 kV was applied to all of the materials, and 99.9 ± 0.2% and 98.8 ± 0.6% virus inactivation efficiencies were confirmed in the cases of carbon and copper, respectively. In addition, an assessment of the effect of flow velocity confirmed that the inactivation efficiency decreased as the flow velocity increased. However, the results for the flow velocities of 0.2 and 0.4 m/s had similar trends. Therefore, this system can be used with flow velocities up to 0.4 m/s.

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Reduction of Escherichia Coli Using Metal Plates with the Influenced of Applied Low Current and Physical Barrier of Filter Layers

Cited by 5 publications

References 31 publications

Effectiveness of copper ions against Mycobacterium avium subsp. paratuberculosis and bacterial communities in naturally contaminated raw cow’s milk

Effectiveness of copper ions against Mycobacterium avium subsp. paratuberculosis and bacterial communities in naturally contaminated raw cow’s milk

More Insights about the Efficacy of Copper Ion Treatment on Mycobacterium avium subsp. paratuberculosis (MAP): A Clue for the Observed Tolerance

Effects of Brush-Type Ionizer Materials on Virus Inactivation

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