Reduction of Mono(ADP-ribosyl)ation of Histones in Rat Testis by Gonadotropin-Testosterone System

Reduced levels of poly(ADP‐ribosyl)ation result in chromatin compaction and hypermethylation as shown by cell‐by‐cell computer‐assisted quantitative analysis

“…Experimental conditions performed to induce the blockage of poly(ADP-ribosyl)ation were the same as those in which the absence of an active poly(ADPribose) polymerase allowed the introduction of new methyl groups into both genomic (16) and CpG islands DNA (17). Since by treating the cells with 8 mM 3-ABA an effect of the mono(ADP-ribosyl)ation process on DNA methylation cannot be excluded (28) in these experiments, unlike those previously reported (16,17), we used a lower 2 mM 3-ABA concentration to specifically inhibit the poly(ADP-ribose) polymerase.…”

Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes

“…Momii and Koide [14] suggested that mouse testicular cells possess an ART that catalyzes the transfer of the ADP-ribose moiety from NAD + to an unknown acceptor protein. In more recent studies, such an enzyme system is also described in rat testes [15]. The authors identified histone H2B and H3 as acceptor proteins and provided evidence that ADP-ribosylation of these proteins was under the control of the gonatropin-testosterone system.…”

Altered molecular dynamics and antioxidant status in the spermatozoa in testosterone-induced oligospermia in mouse

“…Various epididymal secreted proteins which are involved in the maturation of the spermatozoa are androgen dependent (Astraudo et al, 1995;Cyr et al, 1996;Moore and Akhondi, 1996;Sutton et al, 1998;Shivshanmugam et al, 1999). Various enzyme activities and their mRNA expression levels are shown to be affected by testosterone (Kurokawa et al, 1995;Peltola et al, 1996;Viger and Robaire 1996).…”