The article presents the results of the study to analyze the plant and microbial soil associations in the composition of agrocenoses, natural steppe biocenoses and territorially integral areas of grasses on the border or within agrocenoses, mainly anthropogenic origin, named in the article as 'intrusions'. We show the key relationships between the main participants (47 plant species and 24 species of soil microorganisms, ranked by number in the association) in concurrence for the assimilation of soil trace elements. On the basis of data on the number, main phenological characteristics of plants and microorganisms, as well as the content of manganese, nickel, copper and zinc in the soil, the main consort bonds in this biosystem were identified. The analysis of the general influence of concrete intrusions on the adjacent agrocenosis made it possible to identify trace elements and types of soil microorganisms, which increase helped to limit the negative impact of intrusions. The obtained data can be used to form biotechnologies for optimization of agrocenoses in the conditions of the arid zone.