REDV/Rapamycin-loaded polymer combinations as a coordinated strategy to enhance endothelial cells selectivity for a stent system

Yu, Wei; Zhang, Jingxun; Ji, Ying; Ji, Jian

doi:10.1016/j.colsurfb.2015.11.012

Cited by 23 publications

(13 citation statements)

References 33 publications

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“…The REDV sequence can also mediate EC migration on fibronectin via its a4b4 receptor (35). An REDV-functionalized polysaccharide multilayer (36), stent (37), and hyaluronic acid-coated poly(Ecaprolactone) film (32) were successfully verified in selective EC capture and created an EC-binding surface on biomaterials in vitro. However, immobilization of REDV peptides on HEP/COL multilayers to create modified ePTFE films and the endothelialization effectiveness of this modified method had not been reported.…”

Section: Discussionmentioning

confidence: 99%

Application of Heparin/Collagen‐REDV Selective Active Interface on ePTFE Films to Enhance Endothelialization and Anticoagulation

Shan

Jia

et al. 2018

Artificial Organs

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Expanded polytetrafluoroethylene (ePTFE) prosthetic valves have been widely used in clinical applications in Asian countries. However, these valves still have limits with regard to thrombosis, neointimal hyperplasia, restenosis, and valvar vegetation. The achievement of in situ endothelialization on implant materials is a promising way to overcome those limits. Here, heparin/collagen multilayers were fabricated on ePTFE films via a layer-by-layer (LBL) self-assembly technique, and then, the endothelial cell (EC) adhesive peptide sequence Arg-Glu-Asp-Val (REDV) was immobilized on the multilayers. After modification with the heparin/collagen multilayers with or without REDV peptide, less platelet activation and aggregation were observed, the blood coagulation time was increased, and the hemolysis rate was decreased compared to that on pristine ePTFE films. The REDV-functionalized ePTFE films positively impacted early EC adhesion, later cell proliferation and cell activity. The EC barrier was confirmed to be successfully achieved on the functionalized ePTFE film surface in vitro. The successful assembly of the REDV-functionalized heparin/collagen multilayer on ePTFE films improved the blood compatibility, anticoagulant properties, and cell compatibility of the films in vitro, and thus, represents a candidate approach for applications requiring quick in situ endothelialization in vivo.

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Section: Discussionmentioning

confidence: 99%

Application of Heparin/Collagen‐REDV Selective Active Interface on ePTFE Films to Enhance Endothelialization and Anticoagulation

Shan

Jia

et al. 2018

Artificial Organs

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“…It is a promising method to simultaneously immobilize REDV and CAG peptides onto biomaterial surface to selectively adhere ECs due to their different adhesion effects. Depending on the cell‐adhesive peptides, the selective adhesion of ECs over SMCs is an effective strategy to achieve the rapid endothelialization of artificial vascular grafts …”

Section: Introductionmentioning

confidence: 99%

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bai

Zhao

et al. 2018

Macromolecular Bioscience

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Artificial small‐caliber vascular grafts are still limited in clinical application because of thrombosis, restenosis, and occlusion. Herein, a small‐caliber vascular graft (diameter 2 mm) is fabricated from poly(ε‐caprolactone)‐b‐poly(isobutyl‐morpholine‐2,5‐dione) (PCL‐PIBMD) and silk fibroin (SF) by electrospinning technology and then biofunctionalized with low‐fouling poly(ethylene glycol) (PEG) and two cell‐adhesive peptide sequences (CREDVW and CAGW) with the purpose of enhancing antithrombogenic activity and endothelialization. The successful grafting of PEG and peptide sequences is confirmed by X‐ray photoelectron spectroscopy. The suitable surface wettability of the modified vascular graft is testified by water contact angle analysis. The surface hemocompatibility is verified by platelet adhesion assays and protein adsorption assays, and the results demonstrate that both platelet adhesion and protein adsorption on the biofunctionalized surface are significantly reduced. In vitro studies demonstrate that the biofunctionalized surface with suitable hydrophilicity and cell‐adhesive peptides can selectively promote the adhesion, spreading, and proliferation of human umbilical vein endothelial cells. More importantly, compared with control groups, this biofunctionalized small‐caliber vascular graft shows high long‐term patency and endothelialization after 10 weeks of implantation. The biofunctionalization with PEG and two cell‐adhesive peptide sequences is an effective method to improve the endothelialization and long‐term performance of synthetic vascular grafts.

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“…Thus, the stent surface modification using cell membrane bioinspired polymers provides an effective method to solve this problem. 82,195,200,201 For example, drug eluting stents have a simultaneous requirement of a sustained inhibition of the proliferation of smooth muscle cells (SMCs) but a promotion of the endothelial cells (ECs). Ji et al prepared a zwitterionic MPC-based methacrylate coating on the stent surface to load rapamycin and introduced an EC-specific ligand peptide (Arg-Glu-Asp-Val, REDV).…”

Section: Implant Materialsmentioning

confidence: 99%

Bioinspired by cell membranes: functional polymeric materials for biomedical applications

Chen

2020

Mater. Chem. Front.

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REDV/Rapamycin-loaded polymer combinations as a coordinated strategy to enhance endothelial cells selectivity for a stent system

Cited by 23 publications

References 33 publications

Application of Heparin/Collagen‐REDV Selective Active Interface on ePTFE Films to Enhance Endothelialization and Anticoagulation

Application of Heparin/Collagen‐REDV Selective Active Interface on ePTFE Films to Enhance Endothelialization and Anticoagulation

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bioinspired by cell membranes: functional polymeric materials for biomedical applications

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