Regional changes of NADPH-diaphorase and neuropeptide Y neurons in the cerebral cortex of aged Fischer 344 rats

Huh, Youngbuhm; Lee, Wonkyu; Cho, Jungsik; Ahn, Hyung‐Joon

doi:10.1016/s0304-3940(98)00240-7

Cited by 18 publications

(9 citation statements)

References 15 publications

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“…For example, no age-related changes have been observed in the superior colliculus and cerebral cortex (Diaz et al 2006;Huh et al 1997Huh et al , 1998, whereas NO production in the mammalian brain declines with age (Lolova et al 1999;Villena et al 2003;Yamada et al 1996). The present data show that the number of NADPH-d-and nNOS-positive neurons did not change significantly in the DLGN or VLGN of aged rats, but the staining intensities of NADPH-d-nNOS-positive neurons increased significantly in the DLGN and VLGN of older animals.…”

Section: Discussioncontrasting

confidence: 57%

Age-related changes in nitric oxide synthase in the lateral geniculate nucleus of rats

Hwang

Huh

2010

J Mol Hist

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Age-related changes in nitric oxide production in the visual system have not been well characterized. Therefore, we used staining and image-processing approaches to describe changes in levels of neuronal nitric oxide synthase (nNOS), the NADPH-diaphorase (NADPH-d) histochemical marker, and 3-nitrotyrosine in the lateral geniculate nucleus (LGN) of young and aged rats. The LGN plays an important role in the visual system, as it acts as a visual relay nucleus. Quantitative analysis of NADPH-d-positive and nNOS-immunoreactive neurons revealed significant optical density increases in the dorsal LGN and ventral LGN of aged rats; however, no significant changes were observed in the number of neurons with age. 3-Nitrotyrosine immunoreactivity was increased in the dorsal LGN and ventral LGN of aged rats. These results indicate that increased nitric oxide production and peroxynitrite may be associated with alterations in visual function during aging.

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Section: Discussioncontrasting

confidence: 57%

Age-related changes in nitric oxide synthase in the lateral geniculate nucleus of rats

Hwang

Huh

2010

J Mol Hist

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“…However, the number of NPY-IR/NADPH-d-negative neurons significantly decreased in all cerebral cortical areas, except the nucleus accumbens, and the caudatoputamen in the aged group [21]. In a study of aged Fischer 344 rats, which are more resistant to aging than other rats strain [69, 70], the aged group showed that the number of NPY-IR/NADPH-d-positive neurons did also not significantly change in all regions of the cerebral cortex compared to the control group [71]. However, the number of NPY-IR/NADPH-d-negative neurons significantly decreased in the frontal association, primary motor, secondary somatosensory, insular, ectorhinal, perirhinal and auditory cortexes in the aged group [71].…”

Section: Regional Changes In Nadph-d In the Aging Brainmentioning

confidence: 99%

“…In a study of aged Fischer 344 rats, which are more resistant to aging than other rats strain [69, 70], the aged group showed that the number of NPY-IR/NADPH-d-positive neurons did also not significantly change in all regions of the cerebral cortex compared to the control group [71]. However, the number of NPY-IR/NADPH-d-negative neurons significantly decreased in the frontal association, primary motor, secondary somatosensory, insular, ectorhinal, perirhinal and auditory cortexes in the aged group [71]. These studies demonstrated that the NADPH-d containing NPY-IR neurons in the cerebral cortex and striatum of rats were less influenced by aging than those of the control.…”

Section: Regional Changes In Nadph-d In the Aging Brainmentioning

confidence: 99%

Alterations in Nitric Oxide Synthase in the Aged CNS

Jung

Huh

2012

Oxidative Medicine and Cellular Longevity

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Aging is associated with neuronal loss, gross weight reduction of the brain, and glial proliferation in the cortex, all of which lead to functional changes in the brain. It is known that oxidative stress is a critical factor in the pathogenesis of aging; additionally, growing evidence suggests that excessive nitric oxide (NO) production contributes to the aging process. However, it is still unclear how NO plays a role in the aging process. This paper describes age-related changes in the activity of NADPH-diaphorase (NADPH-d), a marker for neurons containing nitric oxide synthase (NOS), in many CNS regions. Understanding these changes may provide a novel perspective in identifying the aging mechanism.

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“…In central auditory structures, NOS activity was found in neuronal elements of the cochlea (Fessenden et al, 1994), superior olivary complex (Reuss, 1998;, inferior colliculus (IC) (Druga and Syka, 1993), and auditory cortex (AC) (Druga and Syka, 1993;Huh et al, 1998;Ouda et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Studies about such changes have been carried out in several parts of the pathway, mostly in its lower steps: cochlea (Kimura et al, 1998) and superior olivary complex , and also the AC (Huh et al, 1998;Ouda et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Age‐related changes of NADPH‐diaphorase‐positive neurons in the rat inferior colliculus and auditory cortex

Sánchez‐Zuriaga

Martí-Gutiérrez

Cruz

et al. 2007

Microscopy Res & Technique

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Nitric oxide (NO) has been implied in age-related changes of the central nervous system (CNS) and the central auditory pathway. The present study was conducted to investigate whether the number of NO-producing cells and their morphometric characteristics in the inferior colliculus (IC) and the auditory cortex (AC) are changed with the increasing age of the subjects. IC and AC sections of adult and senile Wistar rats were studied using the histochemical detection of NADPH-diaphorase activity (NADPH-d), a marker for neurons containing nitric oxide synthase (NOS). Our results showed a decreased area of the somas of NADPH-d-positive neurons in the dorsal cortex (DC) of the IC and a diffuse loss of NADPH-d-positive neurons in the senile IC and primary cortical auditory area (Te1). However, an increased number of NO-producing cells have been shown by other authors in different parts of the ageing auditory pathway and CNS. It seems that age-related changes in NADPH-d-positive cells may follow a region-specific route. These changes may be related to hearing impairments with increasing age.

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Regional changes of NADPH-diaphorase and neuropeptide Y neurons in the cerebral cortex of aged Fischer 344 rats

Cited by 18 publications

References 15 publications

Age-related changes in nitric oxide synthase in the lateral geniculate nucleus of rats

Age-related changes in nitric oxide synthase in the lateral geniculate nucleus of rats

Alterations in Nitric Oxide Synthase in the Aged CNS

Age‐related changes of NADPH‐diaphorase‐positive neurons in the rat inferior colliculus and auditory cortex

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