Regulation of Ionizing Radiation-induced Apoptosis by Mitochondrial NADP+-dependent Isocitrate Dehydrogenase

Lee, Jin Hyup; Kim, Sung Youl; Kil, In Sup; Park, Jeen‐Woo

doi:10.1074/jbc.m700303200

Cited by 79 publications

(43 citation statements)

References 43 publications

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“…Additionally, irradiation stimulates the production of ROS, which induce apoptotic cell death (26). One method of radioprotection involves the inhibition of caspase activation and PARP cleavage (2).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

et al. 2016

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“…Lee et al showed that increased 16 expression of mitochondrial NADP + -dependent isocitrate dehydrogenase (IDPms), which is a key enzyme in the cellular defense against oxidative damage, suppressed γ-irradiation-induced ROS generation and apoptotic cell death [25]. In contrast to these mitochondria-derived ROS, Tateishi In this experiment, to obtain direct evidence that mitochondria are candidates for the organelle source of ROS in tumor cells exposed to X irradiation, we employed the novel fluorescence ROS indicator MitoAR to clarify the localization of ROS production by fluorescent microscopy in living cells and the novel spin-trapping reagent CYPMPO to identify radical species by ESR spectroscopy in a cell-free system.…”

Section: Discussionmentioning

confidence: 99%

Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells

et al. 2009

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Mitochondria in mammalian cells were well-known to play an important role in the intrinsic pathway of genotoxic-agent-induced apoptosis by releasing cytochrome c into cytosol and to be a major source of reactive oxygen species (ROS). The aim of this study is to examine whether mitochondrial ROS involved in radiation-induced apoptotic production from mitochondria to trigger cytochrome c release in A549 cells.

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“…In many cases, the radiation-induced cell death is due to apoptosis Lee et al 2007). Geraniin inhibited apoptosis in cells exposed to γ-radiation as shown by the effects on nuclear fragmentation and loss of Δψ m .…”

Section: Discussionmentioning

confidence: 99%

Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress

et al. 2010

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The radioprotective effect of geraniin, a tannin compound isolated from Nymphaea tetragona Georgi var. (Nymphaeaceae), against γ-radiation-induced damage was investigated in Chinese hamster lung fibroblast (V79-4) cells. Geraniin recovered cell viability detected by MTT test and colony formation assay, which was compromised by γ-radiation, and reduced the γ-radiation-induced apoptosis by the inhibition of loss of the mitochondrial membrane potential. Geraniin protected cellular components (lipid membrane, cellular protein, and DNA) damaged by γ-radiation, which was detected by lipid peroxidation, protein carbonyl formation, and comet assay. Geraniin significantly reduced the level of intracellular reactive oxygen species generated by γ-radiation, which was detected using spectrofluorometer, flow cytometer, and confocal microscope after 2',7'-dichlorodihydrofluorescein diacetate staining. Geraniin normalized the superoxide dismutase and catalase activities, which were decreased by γ-radiation. These results suggest that geraniin protects cells against radiation-induced oxidative stress via enhancing of antioxidant enzyme activities and attenuating of cellular damage.

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Regulation of Ionizing Radiation-induced Apoptosis by Mitochondrial NADP+-dependent Isocitrate Dehydrogenase

Cited by 79 publications

References 43 publications

Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

Protective effects of sodium selenite supplementation against irradiation-induced damage in non-cancerous human esophageal cells

Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells

Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress

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