Regulation of mesenchymal stem cell attachment and spreading on hydroxyapatite by RGD peptides and adsorbed serum proteins

Sawyer, Andrew J.; Hennessy, Kristin M.; Bellis, Susan L.

doi:10.1016/j.biomaterials.2004.05.008

Cited by 196 publications

(197 citation statements)

References 37 publications

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“…When bioceramics are implanted into a living body, proteins from the surrounding body fluids will be spontaneously adsorbed onto their surfaces, and then cellular attachment, proliferation and migration occurs [41][42][43]. Thus, the proteinadsorption behaviour plays a vital role during bone tissue regeneration [44,45].…”

Section: Introductionmentioning

confidence: 99%

A review of protein adsorption on bioceramics

et al. 2012

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Bioceramics, because of its excellent biocompatible and mechanical properties, has always been considered as the most promising materials for hard tissue repair. It is well know that an appropriate cellular response to bioceramics surfaces is essential for tissue regeneration and integration. As the in vivo implants, the implanted bioceramics are immediately coated with proteins from blood and body fluids, and it is through this coated layer that cells sense and respond to foreign implants. Hence, the adsorption of proteins is critical within the sequence of biological activities. However, the biological mechanisms of the interactions of bioceramics and proteins are still not well understood. In this review, we will recapitulate the recent studies on the bioceramic -protein interactions.

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Section: Introductionmentioning

confidence: 99%

A review of protein adsorption on bioceramics

et al. 2012

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“…Published works report the coating or tailoring of biomaterials with bioactive molecules, such as fibronectin [3], laminin [4], growth factors [5,6] or their integrin-binding epitopes including RGD [7,8] and GFOGER [9]. These studies report the influence of such biomolecules in cell adhesion, differentiation or proliferation.…”

Section: Introductionmentioning

confidence: 99%

Selective Cell Recruitment and Spatially Controlled Cell Attachment on Instructive Chitosan Surfaces Functionalized with Antibodies

et al. 2012

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Bioactive constructs to guide cellular mobilization and function have been proposed as an approach for a new generation of biomaterials in functional tissue engineering. Adult mesenchymal stem cells have been widely used as a source for cell based therapeutic strategies, namely tissue engineering. This is a heterogeneous cell population containing many subpopulations with distinct regenerative capacity. Thus, one of the issues for the effective clinical use of stem cells in tissue engineering is the isolation of a highly purified, expandable specific subpopulation of stem cells. Antibody functionalized biomaterials could be promising candidates to isolate and recruit specific cell types. Here we propose a new concept of instructive biomaterials that are able to recruit and purify specific cell types from a mixed cell population. This biomimetic concept uses a target-specific chitosan substrate to capture specific adipose derived stem cells. Specific antibodies were covalently immobilized onto chitosan membranes using bis[sulfosuccinimidyl] suberate (BS3). Quartz crystal microbalance (QCM) was used to monitor antibody immobilization/adsorption onto the chitosan films. Specific antibodies covalently immobilized, kept their bioactivity and captured specific cell types from a mixed cell population. Microcontact printing allowed to covalently immobilize antibodies in patterns and simultaneously a spatial control in cell attachment.

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“…A primary route is the mimicking of mineral-phase hydroxyapatite (HA), which is enabled by the biomimetic synthesis of HA, including low-temperature solution-based methods (Tas, 2000;Moreau et al, 2009). HA with poorly crystallized form has better bone bioactivity, such as protein adsorption, cellular osteogenesis, bone formation, and bone remodeling, due to its higher dissolution properties (Sawyer et al, 2005). However, most synthetic routes involve only ionic-buffered medium in the absence of biological proteins.…”

Section: Advanced Biomatrix Designs Compositional Control To Mimic Namentioning

confidence: 99%

Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

et al. 2014

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Periodontitis is an inflammatory disease that causes loss of the tooth-supporting apparatus, including periodontal ligament, cementum, and alveolar bone. A broad range of treatment options is currently available to restore the structure and function of the periodontal tissues. A regenerative approach, among others, is now considered the most promising paradigm for this purpose, harnessing the unique properties of stem cells. How to make full use of the body's innate regenerative capacity is thus a key issue. While stem cells and bioactive factors are essential components in the regenerative processes, matrices play pivotal roles in recapitulating stem cell functions and potentiating therapeutic actions of bioactive molecules. Moreover, the positions of appropriate bioactive matrices relative to the injury site may stimulate the innate regenerative stem cell populations, removing the need to deliver cells that have been manipulated outside of the body. In this topical review, we update views on advanced designs of biomatrices-including mimicking of the native extracellular matrix, providing mechanical stimulation, activating cell-driven matrices, and delivering bioactive factors in a controllable manner-which are ultimately useful for the regenerative therapy of periodontal tissues.

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Regulation of mesenchymal stem cell attachment and spreading on hydroxyapatite by RGD peptides and adsorbed serum proteins

Cited by 196 publications

References 37 publications

A review of protein adsorption on bioceramics

A review of protein adsorption on bioceramics

Selective Cell Recruitment and Spatially Controlled Cell Attachment on Instructive Chitosan Surfaces Functionalized with Antibodies

Advanced Biomatrix Designs for Regenerative Therapy of Periodontal Tissues

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