Regulation of neuropeptide Y mRNA and peptide concentrations by copper in rat olfactory bulb

Rutkoski, Nancy J.; Fitch, Cheryl A.; Yeiser, E.Carden; Dodge, Janice; Trombley, Paul Q.; Levenson, Cathy W.

doi:10.1016/s0169-328x(98)00345-3

Cited by 15 publications

(8 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Centrally injected NPY induces feeding even in satiated animals. This function appears to be most closely associated with its action in the paraventricular nuclei of the hypothalamus (Rutkoski et al, 1999). Many factors, including Zn and Cu, have been shown to regulate the expression of NPY (Rutkoski et al, 1999;Williamson, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…This function appears to be most closely associated with its action in the paraventricular nuclei of the hypothalamus (Rutkoski et al, 1999). Many factors, including Zn and Cu, have been shown to regulate the expression of NPY (Rutkoski et al, 1999;Williamson, 2002). Although some of the researches that assesses Cu's effects on energy balance have been conducted from the perspective of Cu deficiency, very little work has been done to determine the effects on appetite regulation during excessive Cu administration.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of high dietary copper on the expression of hypothalamic appetite regulators in weanling pigs

Zhu¹,

Yu²,

Ju³

et al. 2011

J. Anim. Feed Sci.

View full text Add to dashboard Cite

The current study evaluated appetite-related gene expression of neuropeptide Y (NPY), orexin, type 2 orexin receptor (OrexinR2), proopiomelanocortin (POMC), melanocortin-4 receptor (MC4R), AMP-activated protein kinase α2 subunit (AMPKα2) and long-form leptin receptor (LeptinRb) in hypothalamus in response to copper (Cu) supplementation. One hundred crossbred pigs were assigned to four groups of 25 pigs, each comprising five replicates of 5 animals. Groups were then randomly assigned to treatments consisted of 1. control (10 mg/kg CuSO 4 ), 2. 100 mg/kg CuSO 4 , 3. 175 mg/kg CuSO 4 , 4. 250 mg/kg CuSO 4 . On d 21 of the experiment 5 pigs from each group were slaughtered and the hypothalami were collected for determination of appetite-regulating genes mRNA expression level. The results showed that average daily feed intake and average daily gain were higher (P<0.05) in 250 and 175, 250 mg/kg Cu supplemented groups, respectively, than in the 10 mg/kg group. Feed:gain ratio was lower in pigs fed the diets with 250 mg/kg Cu (P<0.05) than in the 10 mg/kg group. Furthermore, the abundances of NPY mRNA in hypothalamus were higher in 250 mg/kg Cu supplemented groups (P<0.05), whereas the abundances of POMC and LeptinRb mRNA were significantly reduced (P<0.05) in 170, 250 mg/kg and 100, 175, 250 mg/kg Cu supplemented groups, respectively. No difference was found for AMPKα2, MC4R, orexin, OrexinR2 mRNA expression (P>0.05). These data suggest that down-regulation of LeptinRb mRNA expression might contribute to the stimulation of feeding of high dietary Cu supplementation via regulation of NPY and POMC mRNA expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of high dietary copper on the expression of hypothalamic appetite regulators in weanling pigs

Zhu¹,

Yu²,

Ju³

et al. 2011

J. Anim. Feed Sci.

View full text Add to dashboard Cite

show abstract

“…Copper is required for the proper functioning of several enzymes and has been shown to play an important role in odorant binding for some receptor proteins (Wang et al 2003). Copper is also necessary in the synthesis of certain neuropeptides within the olfactory system (Rutkoski et al 1999) and is believed to be a neuromodulator at various receptor synapses of the olfactory bulb (Trombley and Shepherd 1996;Acuna-Castillo et al 2000;Horning et al 2000). Studies in both mammals and fish have shown that certain heavy metals are actively directed to the olfactory bulb via anterograde axonal transport from the epithelium (Gottofrey and Tjälve 1991;Tallkvist et al 1998Tallkvist et al , 2002.…”

Section: Discussionmentioning

confidence: 99%

Olfactory inhibition and recovery in chum salmon (Oncorhynchus keta) following copper exposure

Sandahl¹,

Miyasaka²,

Koide³

et al. 2006

Can. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Abstract:Copper can be an effective hatchery treatment to control certain parasitic and fungal infections in salmonids, yet it is also highly toxic to the fish's olfactory system. Numerous reports have described the early inhibitory impacts of copper on fish olfaction, but very few have addressed processes of recovery. Here we show that the chum salmon (Oncorhynchus keta) olfactory system can recover from short-term (4 h) exposures to copper (3-58 µgqL -1 ) within 1 day, as indicated by electro-olfactogram (EOG) recordings. Recovery followed initial EOG reductions of up to 88%. We also applied a novel cytophysiological approach to monitor changes in the olfactory receptor cells using a fluorescent styryl dye, AM1-43. Copper's influence on the number of receptor cells that labelled with the dye was biphasic, initially increasing the number at the two lowest copper concentrations (3 and 8 µgqL -1 ) and decreasing the number at the two highest concentrations (24 and 58 µg·L -1 ). Changes in the ability of olfactory receptor neurons to internalize the dye were monitored up to 10 days in fish recovering from copper exposure.Résumé : Le cuivre peut servir de traitement efficace des piscicultures afin de contrôler certaines infections à parasites ou à champignons chez les salmonidés, bien qu'il soit très toxique pour le système olfactif des poissons. De nombreux travaux ont décrit les premiers effets inhibiteurs du cuivre sur l'olfaction des poissons, mais très peu se sont intéressés aux processus de récupération. Nous montrons ici que le système olfactif du saumon kéta (Oncorhynchus keta) peut récupérer après de courtes (4 h) expositions au cuivre (3-58 µg·L -1 ) en moins d'une journée, d'après les enregistrements d'électro-olfactogrammes (EOG). La récupération fait suite à des réductions initiales de l'EOG pouvant atteindre 88 %. Une nouvelle méthode cytophysiologique utilisant un colorant styryle fluorescent, AM1-43, permet de suivre les changements dans les cellules olfactives réceptrices. L'effet du cuivre sur le nombre de cellules réceptrices marquées par le colorant se fait en deux phases, au départ une augmentation du nombre aux deux concentrations les plus faibles de cuivre (3 et 8 µg·L -1 ) et une diminution aux deux concentrations les plus élevées (24 et 58 µg·L -1 ). Nous avons suivi les changements de la capacité des neurones olfactifs récepteurs d'incorporer le colorant jusqu'à 10 jours chez des poissons en train de récupérer d'une exposition au cuivre.[Traduit par la Rédaction] Sandahl et al. 1847

show abstract

“…NPY, a 36 amino acid peptide, is a powerful orexigenic agent, even in satiated animals . The intracerebroventricular administration of NPY could increase short-term (2 h) food intake.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Neuropeptide Y mRNA Expressing Model for Screening Essences That May Affect Appetite Using Rolf B1.T Cells

Chen

Chiang

2012

J. Agric. Food Chem.

View full text Add to dashboard Cite

Neuropeptide Y (NPY) is the most important appetite regulator. This study aimed to establish an in vitro NPY mRNA expression model for screening essences to determine if they are an appetite stimulator or inhibitor. We cultured the olfactory nerve cells Rolf B1.T for 2 days and then treated the cells with the known appetite inhibitor limonene and stimulator linalool. It was found that linalool could significantly stimulate NPY mRNA expression in 10 min, and limonene had the opposite effect. Similar results were also found in primary olfactory ensheathing cells isolated from rats. Further clinical trials using human subjects found that, when 10 min of treatment was applied, linalool indeed increased the serum NPY level in human peripheral blood. Limonene, on the other hand, decreased the serum NPY level. Thus, NPY mRNA expression in Rolf B1.T cells could be used as an in vitro model for screening essences that may affect appetite.

show abstract

Regulation of neuropeptide Y mRNA and peptide concentrations by copper in rat olfactory bulb

Cited by 15 publications

References 43 publications

Effect of high dietary copper on the expression of hypothalamic appetite regulators in weanling pigs

Effect of high dietary copper on the expression of hypothalamic appetite regulators in weanling pigs

Olfactory inhibition and recovery in chum salmon (Oncorhynchus keta) following copper exposure

In Vitro Neuropeptide Y mRNA Expressing Model for Screening Essences That May Affect Appetite Using Rolf B1.T Cells

Contact Info

Product

Resources

About