Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Ma, Xiaoming; La, Yongfu; Bao, Pengjia; Chen, Min; Guo, Xian; Wu, Xiaoyun; Pei, Jie; Ding, Xuezhi; Liang, Chunnian; Yan, Ping

doi:10.3389/fvets.2022.757115

Cited by 6 publications

(6 citation statements)

References 47 publications

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“…In the work, association analysis showed that m6A modification abundance was negatively correlated with gene expression levels, which was consistent with two previous studies [ 51 , 52 ]. We also found that the expression of genes containing more than three m6A sites was much lower than that of genes containing one or two m6A sites, suggesting that m6A can regulate the degradation of mRNA [ 53 ].…”

Section: Discussionsupporting

confidence: 92%

Transcriptome-Wide Study Revealed That N6-Methyladenosine Participates in Regulation Meat Production in Goats

Zou

Shen

Zou

et al. 2023

Foods

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In mammals, skeletal muscle development is a complex biological process regulated by many factors. N6-methyladenosine (m6A) RNA modification plays an important role in many biological processes. However, the regulation of m6A on skeletal muscle growth and development in adult goats remains unclear. In this study, Duan goats (DA) and Nubia goats (NBY), both female and 12 months old, were selected as the research objects, and m6A-Seq and RNA-Seq were mainly used to detect the difference of m6A modification and gene expression during the development of the longissimus dorsi (LD) muscle in the two breeds. The results showed that compared with DA, the meat production performance of NBY was better than that of DA, and the modification level of m6A was higher than that of DA in LD. The m6A-Seq of LD indicated m6A peaks were mainly enriched in the coding sequence (CDS) and stop codon. A total of 161 differentially methylated genes (DMGs) and 1294 differentially expressed genes (DEGs) were identified in two breeds. GO and KEGG analysis showed that DMGs were closely related to cellular metabolism, and most of DMGs were enriched in pathways related to energy metabolism, muscle growth and development, mainly MAPK signaling pathway, Wnt signaling pathway and CGMP-PKG signaling pathway. The DEGs were significantly enriched in actin binding, calcium ion binding, angiogenesis, and other biological processes, and most of them were enriched in PI3K-Akt and CGMP-PKG signaling pathways. Combined analysis of m6A-Seq and RNA-Seq data revealed a negative correlation between differentially methylated m6A levels and mRNA abundance, and mRNA expression of the gene with m6A peak near 3′UTR will decrease. In addition, 11 DMGs regulating cell differentiation, muscle growth and development were identified. This study displayed the m6A profiles and distribution patterns in the goat transcriptome, determined the potential role of m6A modification in muscle growth and provided a new reference for the further study of goat skeletal muscle development.

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Section: Discussionsupporting

confidence: 92%

Transcriptome-Wide Study Revealed That N6-Methyladenosine Participates in Regulation Meat Production in Goats

Zou

Shen

Zou

et al. 2023

Foods

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“…In this study, we identified numerous m 6 A methylation modification sites in both breast and leg tissues of Jingyuan chickens. These m 6 A peaks were primarily concentrated in the 3′UTR, CDS, and Stop codon regions, which aligns with findings from studies on Lexus rabbit and yak ( Luo et al, 2022 ; Ma et al, 2022 ). However, this pattern differed from the findings of Xu et al (2021) in their study on Ding'an goose, where m 6 A was primarily enriched in the CDS and start codon sequence, implying that the pattern of m 6 A modification could be species and tissue specific.…”

Section: Discussionsupporting

confidence: 85%

Regulatory role of N6-methyladenosine in intramuscular fat deposition in chicken

Zhang¹,

Yu²,

Cai³

et al. 2023

Poultry Science

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“…Given their slow growth rate and the low meat yield from yaks, there is a pressing need to expedite their growth in order to enhance meat production. In recent years, several research teams have investigated the transcriptional level mechanism of yak muscle development, providing fundamental insights for further exploration [ 6 , 14 , 37 ]. Building upon previous studies, we used the ATAC-seq and RNA-seq analysis methods for the first time to effectively identify the key factors influencing muscle development from a chromatin accessibility perspective, and explored the potential molecular mechanisms underlying the variations in skeletal muscle growth and development in the Tianzhu white yak.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the substantial involvement of diverse myogenic regulators in skeletal muscle development, epigenetic modifications play a crucial role in facilitating muscle growth [ 8 , 12 , 13 ]. For instance, m6A modification intricately and negatively governs the expression levels of pivotal genes implicated in the growth and development of skeletal muscle in yaks [ 14 ]. The regulation of muscle growth and development is achieved through the methylation of gene promoters, which in turn controls the expression of genes associated with muscle function [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Integration of ATAC-Seq and RNA-Seq Analysis to Identify Key Genes in the Longissimus Dorsi Muscle Development of the Tianzhu White Yak

Li,

Chen,

Bai

et al. 2023

IJMS

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During the postnatal stages, skeletal muscle development undergoes a series of meticulously regulated alterations in gene expression. However, limited studies have employed chromatin accessibility to unravel the underlying molecular mechanisms governing muscle development in yak species. Therefore, we conducted an analysis of both gene expression levels and chromatin accessibility to comprehensively characterize the dynamic genome-wide chromatin accessibility during muscle growth and development in the Tianzhu white yak, thereby elucidating the features of accessible chromatin regions throughout this process. Initially, we compared the differences in chromatin accessibility between two groups and observed that calves exhibited higher levels of chromatin accessibility compared to adult cattle, particularly within ±2 kb of the transcription start site (TSS). In order to investigate the correlation between alterations in chromatin accessible regions and variations in gene expression levels, we employed a combination of ATAC-seq and RNA-seq techniques, leading to the identification of 18 central transcriptional factors (TFs) and 110 key genes with significant effects. Through further analysis, we successfully identified several TFs, including Sp1, YY1, MyoG, MEF2A and MEF2C, as well as a number of candidate genes (ANKRD2, ANKRD1, BTG2 and LMOD3) which may be closely associated with muscle growth and development. Moreover, we constructed an interactive network program encompassing hub TFs and key genes related to muscle growth and development. This innovative approach provided valuable insights into the molecular mechanism underlying skeletal muscle development in the postnatal stages of Tianzhu white yaks while also establishing a solid theoretical foundation for future research on yak muscle development.

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Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Cited by 6 publications

References 47 publications

Transcriptome-Wide Study Revealed That N6-Methyladenosine Participates in Regulation Meat Production in Goats

Transcriptome-Wide Study Revealed That N6-Methyladenosine Participates in Regulation Meat Production in Goats

Regulatory role of N6-methyladenosine in intramuscular fat deposition in chicken

Integration of ATAC-Seq and RNA-Seq Analysis to Identify Key Genes in the Longissimus Dorsi Muscle Development of the Tianzhu White Yak

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