“…Programs that utilize peak alignment algorithms, ascertain measurements for a given peptide in runs where the peptide is not identified at the MS/MS level by leveraging information from other runs, and the development of novel intensity determination and normalization procedures (Cox et al, 2014; May et al, 2008) collectively raised the confidence of researchers in label‐free quantifications based on MS peak area measurements. The availability of the method in commercial (e.g., Progenesis LC‐MS and QI, Proteome Discoverer and Mascot), and in open‐source (MaxQant, https://www.maxquant.org; Tyanova et al, 2016) software packages also has contributed to the widespread application of the technique to retina proteomics (Cehofski et al, 2015; Eastlake et al, 2018; Gharbi et al, 2021; Grotegut et al, 2020; Hauck et al, 2010; B. Liu et al, 2020; Ly et al, 2014; Perumal et al, 2020; Ruzafa et al, 2018; Suo et al, 2022; Tu et al, 2015; Zhu et al, 2017—just to mention a few of these studies).…”