Relation between the particle size distribution and the kinetic performance of packed columns

Billen, Jeroen; Guillarme, Davy; Rudaz, Serge; Veuthey, Jean‐Luc; Ritchie, Harald; Grady, Brian P.; Desmet, Gert

doi:10.1016/j.chroma.2007.05.088

Cited by 48 publications

(33 citation statements)

References 18 publications

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“…As can be seen from Fig. 6a, the particles from [6] follow the same linear relationship between h min and , a trend that was obviously not so clearly visible when a smaller range of PSD widths was investigated.…”

Section: Relation Between Particle Size Distribution and Quality Packmentioning

confidence: 56%

“…From these plots, a clear linear relationship between h min and the width of the PSD is evident. For the sake of interest, the data obtained in [6] have also been added to this figure (open, red symbols) (for interpretation of the references to color in this sentence, the reader is referred to the web version of the article). As can be seen from Fig.…”

Section: Relation Between Particle Size Distribution and Quality Packmentioning

confidence: 99%

“…Another way to assess the packing quality of a support is by using a so-called reduced kinetic plot, plotting the separation impedance (E) versus N/N opt [6,27]. Both measures can be calculated without having to define a characteristic length or diameter, as can be seen in the following equations:…”

Section: Tablementioning

confidence: 99%

“…The width of the PSDs of these columns covers a larger range ( = 0.06-0.21) than the study described above [6]. Although the relation between these measures and the true "packing quality" (whatever that may be) is by no means exact or theoretically sound, we report the value of these commonly used packing quality measures here because these are the parameters that are most often considered by the daily practitioners in the field.…”

Section: Introductionmentioning

confidence: 96%

“…To re-evaluate the results from Dewaele and Verzele [2], a similar study was repeated by mixing two batches of 1.7 and 2.2 m material in different ratios and subsequently evaluating the chromatographic performance of the columns packed with these particle mixtures [6]. These chromatographic results were subsequently compared with the PSDs obtained by Coulter counting measurements.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Relationship between the particle size distribution of commercial fully porous and superficially porous high-performance liquid chromatography column packings and their chromatographic performance

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et al. 2010

Journal of Chromatography A

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Section: Relation Between Particle Size Distribution and Quality Packmentioning

confidence: 56%

Section: Relation Between Particle Size Distribution and Quality Packmentioning

confidence: 99%

Section: Tablementioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Relationship between the particle size distribution of commercial fully porous and superficially porous high-performance liquid chromatography column packings and their chromatographic performance

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Fanigliulo

Bellazzi

et al. 2010

Journal of Chromatography A

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Fundamental to achieving fast separations with high efficiency: A review of chromatography with superficially porous particles

Luo

DeStefano

Langlois

et al. 2021

Biomedical Chromatography

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Types of particles have been fundamental to LC separation technology for many years. Originally, LC columns were packed with large-diameter (>100 μm) calcium carbonate, silica gel, or alumina particles that prohibited fast mobile-phase speeds because of the slow diffusion of sample molecules inside deep pores. During the birth of HPLC in the 1960s, superficially porous particles (SPP, ≥30 μm) were developed as the first high-speed stationary-phase support structures commercialized, which permitted faster mobile-phase flowrates due to the fast movement of sample molecules in/out of the thin shells. These initial SPPs were displaced by smaller totally porous particles (TPP) in the mid-1970s. But SPP history repeated when UHPLC emerged in the 2000s. Stationary-phase support structures made from sub-3-μm SPPs were introduced to chromatographers in 2006. The initial purpose of this modern SPP was to enable chromatographers to achieve fast separations with high efficiency using conventional HPLCs. Later, the introduction of sub-2-μm SPPs with UHPLC instruments pushed the separation speed and efficiency to a very fast zone.This review aims at providing readers a comprehensive and up-to-date view on the advantages of SPP materials over TPPs historically and theoretically from the material science angle.

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Packing density of slurry‐packed capillaries at low aspect ratios

Ehlert

Rösler

Tallarek³

2008

J of Separation Science

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The average interparticle voidage or porosity (epsilon(inter)) in cylindrical capillaries is studied in dependence on the column diameter (d(c)) to particle diameter (d(p)) ratio for 5 < d(c)/d(p) < 50. Using optimized slurry and packing solvents, high pressure and ultrasonication, 5 mum-sized porous C18-silica particles were slurry-packed into fused-silica capillaries having ids from 30 to 250 mum. Packing densities are assessed by a polystyrene standard which is size-excluded from the intraparticle pore space of the packings. For d(c)/d(p) > 35 densely packed beds are realized (epsilon(inter) = 0.36-0.37), while for decreasing aspect ratios an exponential increase in epsilon(inter )is observed reaching epsilon(inter ) approximately 0.47 at d(c)/d(p) = 5. This behaviour is ascribed to a combination of the geometrical wall effect operating in the direct vicinity of the column wall, caused by the inability of the particles to form a dense packing against the hard surface of the column wall, and particle characteristics like the size distribution, shape and surface roughness. Results are compared with the literature data to address also the importance of absolute particle size in studying structure-transport relations in packed beds in dependence on the aspect ratio d(c)/d(p).

show abstract

Relation between the particle size distribution and the kinetic performance of packed columns

Cited by 48 publications

References 18 publications

Relationship between the particle size distribution of commercial fully porous and superficially porous high-performance liquid chromatography column packings and their chromatographic performance

Relationship between the particle size distribution of commercial fully porous and superficially porous high-performance liquid chromatography column packings and their chromatographic performance

Fundamental to achieving fast separations with high efficiency: A review of chromatography with superficially porous particles

Packing density of slurry‐packed capillaries at low aspect ratios

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