Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in Southern China

Li, Chunxiao; Kaufman, Phillip E.; Xue, Rui‐De; Zhao, Mingzhu; Wang, Gang; Yan, Tingting; Guo, Xiaoxia; Zhang, Yingmei; Dong, Yan-De; Xing, Dan; Zhang, Heng-Duan; Zhao, Tongyan

doi:10.1186/s13071-015-0933-z

Cited by 117 publications

(134 citation statements)

References 29 publications

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“…The present study reveals the co-occurrence of L1014S and L1014F mutations coupled with high insecticide resistance in the two Anopheline populations belonging to agricultural and residential settings in northwest Nigeria suggesting the spreading of the L1014S mutation gene across Africa. The high allelic frequencies recorded in the residential sites could be due to the increased use of pyrethroids for ITNs and IRS in public health as indicated in previous studies (Dykes et al 2015;Li et al 2015). The co-occurrence of the L1014S and L1014F mutations has already been reported in An.…”

Section: Resultsmentioning

confidence: 59%

Malaria vectors resistance to commonly used insecticides in the control of malaria in Bichi, northern Nigeria

Habibu¹,

Andrew²,

Hapca³

et al. 2018

Bayero J. Pure App. Sci.

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This study was carried out in 2015 to assess the level of resistance of sibling species of Anopheles gambiae complex the principal malaria vector from Bichi in Kano state to three classes of insecticides; (DDT, Permethrin and Bendiocarb) approved by World Health Organization (WHO) for vector control with the aim of obtaining baseline susceptibility and resistance data by evaluating the potency of brands of insecticides used against the mosquito vector in the area where the disease is prevalent. An. gambiae larvae collected from ecologically contrasting breeding sites were reared to adults in the laboratory. Adults from the F1 progeny were assayed for resistance using the WHO adult insecticide susceptibility bioassay protocol. The mosquitoes were exposed to DDT, permethrin and bendiocarb belonging to 3 classes of insecticides. The individual sibling species were identified molecularly and the molecular resistance mechanisms characterized by SINE and kdr PCR methods respectively. The An. gambiae populations tested revealed high level of resistance to DDT and permethrin but less resistant to bendiocarb. Analysis of the genetic composition of the vector population revealed predominance of An. coluzzii (> 77%). L1014F and L1014S kdr mutations correlated to insecticide resistance phenotype expression. This study indicates differential distribution of the resistant genotype of Anopheles malaria vector between ecologically different habitats in the area. This insecticide resistance represents a threat to vector control tool and should be monitored. The information obtained could be useful in decision and planning making for vector control programs in the region.

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Section: Resultsmentioning

confidence: 59%

Malaria vectors resistance to commonly used insecticides in the control of malaria in Bichi, northern Nigeria

Habibu¹,

Andrew²,

Hapca³

et al. 2018

Bayero J. Pure App. Sci.

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“…In Asia, V1016G has been found with S989P in Thailand and Malaysia, V1016G and F1534C in Singapore, S989P, V1016G and F1534C in China, Thailand, Myanmar, Saudi Arabia and Indonesia, and T1520I with F1534C in India. 14,15,[29][30][31][32][33][34] In the Americas, the co-occurrence of V1016I and F1534C has been found in the Caribbean Islands, Mexico, Brazil, Venezuela and the USA; in Africa, the co-occurrence of V1016I and F1534C in Ghana has been reported. 31,[35][36][37][38][39][40][41] In conclusion, we report here the co-occurrence of mutations V1016I and F1534C in populations of A. aegypti of the Colombian Caribbean region with variation in its allelic and genotypic frequencies.…”

Section: Discussionmentioning

confidence: 99%

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Maestre‐Serrano

Pareja-Loaiza

Camargo

et al. 2019

Pest Management Science

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BACKGROUND Knockdown resistance is conferred primarily by non‐synonymous mutations that reduce pyrethroids binding to voltage‐gated sodium channels. In 2014, kdr mutation V1016I in Aedes aegypti populations resistant to pyrethroids was reported for the first time in Colombiα, in 2016 another kdr mutation, F1534C, and in 2018 the mutation V419L. Nine populations of A. aegypti, previously characterized as being resistant to λ‐cyhalothrin, deltamethrin, cyfluthrin and permethrin, were used for this study. Genomic DNA was used to determine genotypes by allele‐specific PCR for mutations V1016I and F1534C, and to determine their association with pyrethroid resistance. RESULTS All the populations analyzed showed both mutations, with allelic frequencies of 0.07–0.35 for I1016 and 0.47–0.88 for C1534. A percentage of co‐occurrence of mutant homozygotes I1016/C1534 of 5.3% was detected. A significant positive correlation was found between the frequency of the genotype I1016 and the resistance to permethrin, λ‐cyhalothrin and cyfluthrin, but not to deltamethrin; on the other hand, the correlation was not significant for the C1534 genotype and the four pyrethroids evaluated. No significant correlation was found between the frequencies of the mutations V1016I and F1534C. CONCLUSIONS Both mutations V1016I and F1534C are present in A. aegypti populations of the Colombian Caribbean, and although the frequency of F1534C exceeds V1016I, the latter was correlated to resistance to pyrethroid insecticides. © 2018 Society of Chemical Industry

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“…This haplotype is commonly found in Ae. aegypti [6, 13, 16, 17, 33] but not in South America where the haplotype V1016I is mainly dominating [34]. Conversely, the haplotype F1534C is much less frequently found in Aedes populations when compared to V1016G and S989P genes.…”

Section: Discussionmentioning

confidence: 99%

Knockdown resistance (kdr) of the voltage-gated sodium channel gene of Aedes aegypti population in Denpasar, Bali, Indonesia

et al. 2017

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Background Aedes aegypti is the main vector of several arthropod-borne viral infections in the tropics profoundly affecting humans, such as dengue fever (DF), West Nile (WN), chikungunya and more recently Zika. Eradication of Aedes still largely depends on insecticides, which is the most cost-effective strategy, and often inefficient due to resistance development in exposed Aedes populations. We here conducted a study of Ae. aegypti resistance towards several insecticides regularly used in the city of Denpasar, Bali, Indonesia.Methods Aedes aegypti egg samples were collected with ovitraps and thereafter hatched in the insectary of the Gadjah Mada University. The F0 generation was used for all bioassay-related experiments and knockdown resistance (kdr) assays.ResultsResults clearly showed resistance development of Ae. aegypti against tested insecticides. Mortalities of Ae. aegypti were less than 90% with highest resistance observed against 0.75% permethrin. Mosquitoes from the southern parts of Denpasar presented high level of resistance pattern in comparison to those from the western and northern parts of Denpasar. Kdr analysis of voltage-gated sodium channel (Vgsc) gene showed significant association to S989P and V1016G mutations linked to resistance phenotypes against 0.75% permethrin. Conversely, Ae. aegypti F1534C gene mutation did not result in any significant correlation to resistance development.ConclusionsPeriodically surveillance of insecticide resistances in Ae. aegypti mosquitoes will help local public health authorities to set better goals and allow proper evaluation of on-going mosquito control strategies. Initial detection of insecticide resistance will contribute to conduct proper actions in delaying mosquito resistance development such as insecticide rotation or combination of compounds in order to prolong chemical efficacy in combating Ae. aegypti vectors in Indonesia.

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Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in Southern China

Cited by 117 publications

References 29 publications

Malaria vectors resistance to commonly used insecticides in the control of malaria in Bichi, northern Nigeria

Malaria vectors resistance to commonly used insecticides in the control of malaria in Bichi, northern Nigeria

Co‐occurrence of V1016I and F1534C mutations in the voltage‐gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region

Knockdown resistance (kdr) of the voltage-gated sodium channel gene of Aedes aegypti population in Denpasar, Bali, Indonesia

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