Relative expressions of miR-205-5p, miR-205-3p, and miR-21 in tissues and serum of non-small cell lung cancer patients

Jiang, Min; Zhang, Peng; Hu, Guozhu; Xiao, Zuke; Xu, Fang; Zhong, Ting; Huang, Fang; Kuang, Haibin; Zhang, Wei

doi:10.1007/s11010-013-1755-y

Cited by 67 publications

(56 citation statements)

References 16 publications

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“…Previously, the deregulation of miRs has been reported to be associated with the development and advancement of NSCLC (7,8). Among these miRs, miR-133b has been indicated to inhibit NSCLC growth via targeting epidermal growth factor receptor, suggesting that miR-133b may be a tumor suppressor in NSCLC (9).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-133b inhibits the migration and invasion of non small cell lung cancer cells via targeting FSCN1

et al. 2016

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Abstract. MicroRNA (miR)-133b has been reported to act as a tumor suppressor in multiple types of human cancers, including non small cell lung cancer (NSCLC). However, the underlying mechanism by which miR-133b inhibits NSCLC metastasis remains largely unclear. In the present study, reverse transcription-quantitative polymerase chain reaction and western blotting were used to detect messenger RNA and protein expression. A wound healing assay and transwell assay were used to examine the cell migration and invasion. The expression level of miR-133b was found to be significantly downregulated in NSCLC cell lines compared with normal lung epithelial BEAS-2B cells. Further investigation identified fascin1 (FSCN1) as a direct target of miR-133b in NSCLC cells. The expression of FSCN1 was significantly increased in NSCLC cell lines compared with BEAS-2B cells, and its protein expression was negatively regulated by miR-133b in NSCLC A549 cells. Further investigation showed that the upregulation of miR-133b notably inhibited NSCLC cell migration and invasion, while the overexpression of FSCN1 significantly promoted NSCLC cell migration and invasion. Furthermore, the overexpression of FSCN1 reversed the suppressive effect of miR-133b overexpression on NSCLC cell migration and invasion. Accordingly, the present study suggests that miR-133b inhibits the migration and invasion of NSCLC cells via directly targeting FSCN1, and thus may be used for the treatment of NSCLC metastasis.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-133b inhibits the migration and invasion of non small cell lung cancer cells via targeting FSCN1

et al. 2016

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“…[17] Hsa-miR-205-3p, which was downregulated to the greatest extent in the present study, was reported to be significantly upregulated in the tissues and serum of non-small cell lung carcinoma (NSCLC) patients compared with the control groups, indicating that miR-205-3p may be a valuable biomarker for diagnosis and prognosis of NSCLC patients. [18] Overall, the modification of miRNA expression profiles may imply the potential roles of miRNAs in the anticancer effects of EGCG on NPC.…”

Section: Discussionmentioning

confidence: 99%

Epigallocatechin-3-gallate Modulates MicroRNA Expression Profiles in Human Nasopharyngeal Carcinoma CNE2 Cells

Li¹,

Huang

et al. 2017

Chinese Medical Journal

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Background:Epigallocatechin-3-gallate (EGCG) has exhibited antitumor properties in several types of cancers, including nasopharyngeal carcinoma (NPC), but the molecular mechanisms underlying this function remain incompletely understood. The aim of the present study was to characterize the global impact of EGCG on the expression of microRNAs (miRNAs) in NPC cells.Methods:Using microarray analysis, the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells. Furthermore, the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis.Results:A total of 14 miRNAs exhibited >2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG. Totally 43, 49, and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis, cell cycle regulation, and cell proliferation, respectively. A total of 66 signaling pathways, primarily involved in cancer development and lipid and glucose metabolism, were shown to be regulated by EGCG-specific miRNAs.Conclusion:EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells, which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG.

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“…A recent study used high-throughput microarrays to measure miRNA expression in 122 adenocarcinoma and squamous cell carcinoma (SCC) specimens and demonstrated that the expression of hsa-miR-205 in malignant tissues is an accurate marker of SCC lung cancer (Lebanony et al, 2009). Furthermore, the relative expression of miR-205-5p was significantly higher in non-small cell lung cancer tissues compared with non-cancer adjacent tissues paired specimens (Jiang et al, 2013). Consistent with these observations, our findings displayed an increased miR-205-5p expression in NPC tissues.…”

Section: Discussionmentioning

confidence: 99%

Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

Tang¹,

Zhong²,

Li³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.

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Relative expressions of miR-205-5p, miR-205-3p, and miR-21 in tissues and serum of non-small cell lung cancer patients

Cited by 67 publications

References 16 publications

MicroRNA-133b inhibits the migration and invasion of non small cell lung cancer cells via targeting FSCN1

MicroRNA-133b inhibits the migration and invasion of non small cell lung cancer cells via targeting FSCN1

Epigallocatechin-3-gallate Modulates MicroRNA Expression Profiles in Human Nasopharyngeal Carcinoma CNE2 Cells

Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

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