Reproducible Design for the Optical Screening and  Sensing of Hg(II) Ions

Elshehy, Emad A.; El‐Safty, Sherif A.; Shenashen, Mohamed A.

doi:10.3390/chemosensors2040219

Cited by 15 publications

(5 citation statements)

References 60 publications

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“…This behavior is related to the microporous network formation. [44][45][46][47] Figure 2D shows the NLDFT-pore distribution of S-CNS-1 (violet line) and S-CNS-2 (green line), confirming the N2-adsorption data for the presence of microporous particles with pore size ranging from 1.5 nm to 2.2 nm. The surface areas (SBET) of S-CNS-1 and S-CNS-2 are 612 and 164 m 2 g -1 , respectively.…”

Section: Controlled Formation Of 3d Circular S-doped Carbon Spheroidal Surfacessupporting

confidence: 69%

“…The N 2 adsorption behavior displays a typical type I isotherm, in which a sharp uptake at low P / P 0 ( P / P 0 = 0.02) and high uptake at a high relative pressure ( P / P 0 > 0.9) were observed. This behavior is related to the formation of a microporous network. − Figure D shows the pore size distribution curves of S-CSN materials that are determined by using nonlocal density functional theory (NLDFT)-pore distribution of S-CNS-1 (violet line) and S-CNS-2 (green line), confirming the presence of microporous particles with pore sizes ranging from 1.5 to 2.2 nm. The surface areas ( S BET ) of S-CNS-1 and S-CNS-2 are 612 and 164 m 2 g –1 , respectively.…”

Section: Results and Discussionmentioning

confidence: 75%

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Three-Dimensional Circular Surface Curvature of a Spherule-Based Electrode for Selective Signaling and Dynamic Mobility of Norepinephrine in Living Cells

Emran

Shenashen

El‐Safty

et al. 2020

ACS Appl. Bio Mater.

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A highly sensitive protocol for signaling norepinephrine (NEP) in human fluids and neuronal cell line models should be established for clinical investigation of some neuronal diseases. A metal-free electrode catalyst was designed based on sulfur-doped carbon spheroidal surface (S-CSN) and employed as a transducing element for selective signaling of NEP in biological samples. The designed electrode of S-CSN features spherical construct and curvature surface to form spheroidal nanolayer with an average layer size of < 2 nm. The S-CSN shows surface topography of circular surface curvature with rugged surface texture, ridge end, and free open spaces between interlayers. The rich-space diversity surfaces offer forest electron transports and heavily target loads along with in-/out-plane circular spheres of the S-CSN surface. The active doping of the carbon-based electrode by S atoms creates an active transducing element with many active sites, strong binding to targeted molecules, facile diffusion of charges/molecules, long-term durability, and dense reactive exposure sites for signaling NEP at ultra-trace levels. S-CSN could be a sensitive and selective nanosensor for signaling NEP and establishing a sensing protocol with high stability and reproducibility. The sensory protocol based on S-CSN exhibits high sensitivity and selectivity with a low detection limit of 0.001 µM and a wide linear range of 0.01-0.8 µM. The in vitro sensory protocol of NEP secreted from living cells (neuronal cell line model) under stimulated agents possesses high sensitivity, low cytotoxicity, and high biocompatibility. These results confirm the successful establishment of NEP sensor in human blood samples and neuronal cells for clinical investigation.

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Section: Controlled Formation Of 3d Circular S-doped Carbon Spheroidal Surfacessupporting

confidence: 69%

Section: Results and Discussionmentioning

confidence: 75%

Three-Dimensional Circular Surface Curvature of a Spherule-Based Electrode for Selective Signaling and Dynamic Mobility of Norepinephrine in Living Cells

Emran

Shenashen

El‐Safty

et al. 2020

ACS Appl. Bio Mater.

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“…Such results demonstrate that the immobilization of Hb onto the Ni foam electrode is a promising non-enzymatic H2O2 sensor. The specific utility of this sensor design was interesting for its application as molecular and ion species sensing tools in environmental samples and physiological fluids [47][48][49][50][51].…”

Section: Discussionmentioning

confidence: 99%

Simple and Sensitive Electrochemical Sensor-Based Three-Dimensional Porous Ni-Hemoglobin Composite Electrode

2014

Self Cite

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The development of sensing systems that can detect ultra-trace amounts of hydrogen peroxide (H2O2) remains a key challenge in biological and biomedical fields. In the present study, we introduce a simple and highly sensitive enzymeless H2O2 biosensor based on a three-dimensional open pore nickel (Ni) foam electrode functionalized with hemoglobin (Hb). Our findings revealed that the Hb maintained its biological functions and effective electronic connection even after immobilization process. The exceptional physical and intrinsic catalytic properties of the Ni foam combined with the bio-functionality and electron transport facility of the Hb robustly construct a H2O2 biosensor. The enzymeless H2O2 biosensor showed high selectivity, a quick response time, high sensitivity, a wide linear range and a low limit of detection (0.83 μM at a signal-to-noise ratio of three). Such an electrode composition with safe immobilization processes offers viability for engineering new biosensors.

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“…Limit of detection (LOD) for the developed sensor was calculated with the formula LOD = (k * sb)/m, where k = 2 for 92.1 confident level, sb is standard deviation of blank, and m is the slope of the linear response curve [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

Ertekin

Öztürk

2016

Sensors

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This study introduces the use of an IgA isotype aflatoxin (AF) specific monoclonal antibody for the development of a highly sensitive Quartz Crystal Microbalance (QCM) immunobiosensor for the detection of AF in inhibitory immunoassay format. The higher molecular weight of IgA antibodies proved an advantage over commonly used IgG antibodies in label free immunobiosensor measurements. IgA and IgG antibodies with similar affinity for AF were used in the comparative studies. Sensor surface was prepared by covalent immobilization of AFB1, using self assembled monolayer (SAM) formed on gold coated Quartz Crystal, with 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxy succinimide (EDC/NHS) method using a diamine linker. Nonspecific binding to the surface was decreased by minimizing the duration of EDC/NHS activation. Sensor surface was chemically blocked after AF immobilization without any need for protein blocking. This protein free sensor chip endured harsh solutions with strong ionic detergent at high pH, which is required for the regeneration of the high affinity antibody-antigen interaction. According to the obtained results, the detection range with IgA antibodies was higher than IgG antibodies in QCM immunosensor developed for AFB1.

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Reproducible Design for the Optical Screening and Sensing of Hg(II) Ions

Cited by 15 publications

References 60 publications

Three-Dimensional Circular Surface Curvature of a Spherule-Based Electrode for Selective Signaling and Dynamic Mobility of Norepinephrine in Living Cells

Three-Dimensional Circular Surface Curvature of a Spherule-Based Electrode for Selective Signaling and Dynamic Mobility of Norepinephrine in Living Cells

Simple and Sensitive Electrochemical Sensor-Based Three-Dimensional Porous Ni-Hemoglobin Composite Electrode

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

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