Resistance to barley yellow dwarf luteovirus in Aegilops species

Abstract

One thousand and ninety-seven Aegilops accessions were evaluated for their reaction to a PAV serotype of barley yellow dwarf luteovirus (BYDV). The accessions tested belong to the species bicornis, biuncialis, caudata, crassa, columnaris, comosa, cylindrica, kotschyi, longissima, mutica, neglecta (= triaristata 4 ×), ovata, peregrina, searsii, sharonensis, speltoides, tauschii (= squarrosa), triuncialis, umbellulata, uniaristata, vavilovii and ventricosa. The first evaluation of virus levels in the different accessions was conducted at International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria, using double antibody sandwich ELISA (DAS-ELISA). Accession reaction ranged from highly resistant to highly susceptible. Thirty-eight Aegilops accessions resistant at ICARDA, were evaluated at Sainte-Foy, Quebec, Canada, by tissue-blot immunoassay. Diversity of response to BYDV infection was again observed in this elite group. Seven accessions belonging to the species biuncialis, caudata, neglecta and triuncialis were highly BYDV resistant at both locations; five of these originated from Bulgaria. Key words: Introgression, interspecific, Triticum aestivum, BYDV, ELISA, immunoassay, tissue blot

Methodology Molecular cytogenetic characterization of the Aegilops biuncialis karyotype

“…Some A. biuncialis accessions are highly resistant to barley yellow dwarf disease (Makkouk et al, 1994), and others have superior rust resistance (Dhaliwal et al, 2002). A. biuncialis genotypes from dry environments generally have a drought tolerance better than that of wheat.…”

GISH reveals different levels of meiotic pairing with wheat for individual Aegilops biuncialis chromosomes

“…(2n=4x=28; U b U b M b M b ) is an annual allotetraploid species with good tolerance of drought stress (Molnár et al 2004), barley yellow dwarf luteovirus (Makkouk et al 1994), yellow rust and brown rust (Damania andPecetti 1990, Dimov et al 1993), which would be desirable to transfer into cultivated wheat. Triticum aestivum-Ae.…”

Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

“…(Kimber and Sears 1983;Badaeva et al 2004). Aegilops biuncialis has good tolerance against biotic (Damania and Pecetti 1990;Makkouk et al 1994) and abiotic stresses such as cold and salt stress (Colmer et al 2006). Accessions originating from semi-desert habitats can also be used as gene sources to improve drought and heat tolerance of wheat (T. aestivum L.) (Molnár et al 2004;Dulai et al 2005).…”