Resolution of ternary mixtures of Tartrazine, Sunset yellow and Ponceau 4R by derivative spectrophotometric ratio spectrum-zero crossing method in commercial foods

Nevado, J. J. Berzas; Rodríguez, José Manuel Fernández; Cabanillas, Carmen Guiberteau; Llerena, M. J. Villaseñor; Salcedo, A.M. Contento

doi:10.1016/s0039-9140(97)00348-2

Cited by 74 publications

(25 citation statements)

References 8 publications

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“…Os tempos de retenção obtidos foram 10,0 e 17,4 min para os corantes tartrazina e amarelo crepúsculo, respectivamente [21][22][23] .…”

Section: Procedimento Cromatográficounclassified

“…A derivatização dos espectros permite separar sinais sobrepostos e eliminar "background" causado pela presença de outras espé-cies na amostra. Portanto, torna-se desnecessária uma etapa preliminar de separação das espécies a serem determinadas, simplificando o procedimento analítico e aumentando a velocidade analítica 17,18 . A espectrofotometria derivativa consiste na representação da razão da variação da absorvância com o comprimento de onda, em função do comprimento de onda.…”

Section: Introductionunclassified

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Espectrofotometria derivativa: uma estratégia simples para a determinação simultânea de corantes em alimentos

Vidotti¹,

Rollemberg²

2006

Quím. Nova

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Recebido em 17/1/05; aceito em 22/7/05; publicado na web em 13/1/06 DERIVATIVE SPECTROPHOTOMETRY: A SIMPLE STRATEGY FOR SIMULTANEOUS DETERMINATION OF FOOD DYES. A very simple spectrophotometric method is described for resolving binary mixture of the food colorants Sunset Yellow (INS 110) and Tartrazine Yellow (INS 102) by using the first derivative spectra with measurements at zero-crossing wavelengths. Before the spectrophotometric measurements, the dyes were sorbed onto polyurethane foam and recovered in N,N-dimethilformamide. Commercial food products (gelatine and juice powder) were analysed by using the proposed method and the HPLC technique. The results are in very good agreement and the differences between the methods is not statistically important. Therefore, the first-order derivative spectrophotometric method is accurate, precise, reliable and could be applied to the routine analysis of food samples.Keywords: first derivative spectrophotometry; polyurethane foam; food dyes. INTRODUÇÃOMuitos produtos alimentícios originalmente não apresentam cor, e em outros a cor é perdida ou alterada durante o processo de industrialização; assim, surge a necessidade da adição de corantes a fim de conferir ou restaurar a coloração perdida 1 . Os corantes amarelo crepúsculo e amarelo tartrazina são corantes sintéticos que apresentam estruturas distintas das substâncias corantes encontradas na natureza (Figura 1). Estes corantes são comumente adicionados aos alimentos, proporcionando coloração intensa e estável ao produto industrializado. Assim como para muitos outros aditivos alimentares, o controle analítico dos corantes sintéticos é de grande importância na indústria alimentí-cia, devido ao potencial tóxico e carcinogênico que apresentam 2-4 .Muitos métodos analíticos têm sido descritos para determinar misturas de corantes em alimentos, incluindo cromatografia 5 , espectrofotometria 6-8 , voltametria 9-11 , eletroforese capilar 12 e vá-rias combinações de técnicas 13,14 . Os métodos baseados no uso da espectrofotometria derivativa têm apresentado resultados satisfatórios na determinação de misturas de corantes em alimentos 15,16 . A espectrofotometria derivativa é, atualmente uma ferramenta analítica adicional que auxilia na resolução de diversos problemas analíticos. A derivatização dos espectros permite separar sinais sobrepostos e eliminar "background" causado pela presença de outras espé-cies na amostra. Portanto, torna-se desnecessária uma etapa preliminar de separação das espécies a serem determinadas, simplificando o procedimento analítico e aumentando a velocidade analítica 17,18 . A espectrofotometria derivativa consiste na representação da razão da variação da absorvância com o comprimento de onda, em função do comprimento de onda. A diferenciação da lei de LambertBeer (d n A/dλ n = cb(d n ε/dλ n )) mostra que as derivadas são sempre proporcionais às concentrações do analito, sendo as aplicações analíticas baseadas nesta relação 19 . O objetivo do presente trabalho é determinar simultaneamente os corantes a...

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“…Os tempos de retenção obtidos foram 10,0 e 17,4 min para os corantes tartrazina e amarelo crepúsculo, respectivamente [21][22][23] .…”

Section: Procedimento Cromatográficounclassified

Section: Introductionunclassified

Espectrofotometria derivativa: uma estratégia simples para a determinação simultânea de corantes em alimentos

Vidotti¹,

Rollemberg²

2006

Quím. Nova

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“…Different analytical methods have been developed for the qualitative and quantitative analysis of food dyes, chromatography, spectrometry and voltammetery, or various combinations of them [9][10][11][12][13][14]. Chromatographic techniques have the disadvantage of requiring expensive equipment and expert operators.…”

Section: Introductionmentioning

confidence: 99%

Novel PVC-based coated graphite electrode for selective determination of quinoline yellow

Rouhani

Haji-ghasemi

2009

JICS

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Novel coated graphite electrode (CGE) for the determination of quinoline yellow (QY), an artificial food colorant, was evaluated based on the use of tetraphenylphosponium-QY ion-pair complex as the electroactive substance. The electrode revealed linear emf-pQY response over wide concentration ranges (1.0 × 10 -1 ~ 5.0 × 10 -5 M ) with a slope -30.1 ± 0.2 mV decade -1 and a limit of detection of 4.0 × 10 -5 M. The electrode showed good stability, reproducibility and fast response (<5 s). It can be used in wide pH range (4.7-10.7) and was successfully applied to determination of quinoline yellow in artificial mixtures and commercial soft drinks. The results obtained with the electrode were in good agreement with the value obtained by using HPLC measurements, as an official method.

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“…For this reason, simple and rapid methods are required for their determination in food. Thus, methods based on absorbance measurements such as derivative spectrophotometry [7,8] have been reported, but they are suitable for analyzing few-component mixtures. Moreover, electrochemical (EC) methods such as differential pulse polarography [9,10], square wave adsorptive voltammetry [11], and differential pulse stripping voltammetry [12,13] were adopted for the determination of azo-compounds [14].…”

Section: Introductionmentioning

confidence: 99%

Rapid analysis of azo‐dyes in food by microchip electrophoresis with electrochemical detection

et al. 2007

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A method based on microchip electrophoresis with electrochemical (EC) detection has been developed for the simultaneous determination of Yellow AB, Red 2G, Sunset Yellow, New Coccine, and Amaranth which are azo-dyes frequently added to foodstuffs. Factors affecting both separation and detection processes were examined and optimized, with best performance achieved by using a 10 mM phosphate buffer (pH 11) as BGE solution and applying a voltage of 2500 V both in the separation and in the electrokinetic injection (duration 4 s). Under these optimal conditions, the target dye analytes could be separated and detected within 300 s by applying a detection potential of -1.0 V (vs. Ag/AgCl) to the glassy carbon (GC) working electrode. The recorded peaks were characterized by a good repeatability (RSD=1.8-3.2%), high sensitivity, and a wide linear range. Detection limits of 3.8, 3.4, 3.6, 9.1, 15.1 microM were obtained for Yellow AB, Red 2G, Sunset Yellow, New Coccine, and Amaranth, respectively. Fast, sensitive, and selective response makes the new microchip protocol very attractive for the quantitative analysis of commercial soft drinks and candies.

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Resolution of ternary mixtures of Tartrazine, Sunset yellow and Ponceau 4R by derivative spectrophotometric ratio spectrum-zero crossing method in commercial foods

Cited by 74 publications

References 8 publications

Espectrofotometria derivativa: uma estratégia simples para a determinação simultânea de corantes em alimentos

Espectrofotometria derivativa: uma estratégia simples para a determinação simultânea de corantes em alimentos

Novel PVC-based coated graphite electrode for selective determination of quinoline yellow

Rapid analysis of azo‐dyes in food by microchip electrophoresis with electrochemical detection

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