Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L.

Abstract

An F2 population of pea (Pisum sativum L.) consisting of 174 plants was analysed by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. Ascochyta pisi race C resistance, plant height, flowering earliness and number of nodes were measured in order to map the genes responsible for their variation. We have constructed a partial linkage map including 3 morphological character genes, 4 disease resistance genes, 56 RFLP loci, 4 microsatellite loci and 2 RAPD loci. Molecular markers linked to each resistance gene were found: Fusarium wilt (6 cM from Fw), powdery mildew (11 cM from er) and pea common Mosaic virus (15 cM from mo). QTLs (quantitative traits loci) for Ascochyta pisi race C resistance were mapped, with most of the variation explained by only three chromosomal regions. The QTL with the largest effect, on chromosome 4, was also mapped using a qualitative, Mendelian approach. Another QTL displayed a transgressive segregation, i.e. the parental line that was susceptible to Ascochyta blight had a resistance allele at this QTL. Analysis of correlations between developmental traits in terms of QTL effects and positions suggested a common genetic control of the number of nodes and earliness, and a loose relationship between these traits and height.

QTL identification for early blight resistance (Alternaria solani) in a Solanum lycopersicum × S. arcanum cross

“…For EB resistance in tomato, Zhang et al (2003) also detected a QTL on chromosome 3 for which the resistance allele was inherited from the susceptible parent. The presence of QTL with effects opposite to those predicted by the parents may be responsible for the occurrence of individuals with transgressive phenotypes (De Vicente and Tanksley 1993;Dirlewanger et al 1994). Notwithstanding the differences in experimental techniques (pathogen isolates, inoculation method, and resistance assessment criteria) and environmental conditions between the disease tests, we detected three EB QTL in the glasshouse (chromosomes 2, 5, and 9), which coincided with QTL for resistance traits in the field.…”

Translational Genomics in Agriculture: Some Examples in Grain Legumes

“…Resistance to biotic stresses Angular leaf spot QTL 22-64 Oblessuc et al, 2012;Teixeira et al, 2005 (Khedikar et al, 2010;Liang et al, 2009a;Qin et al, 2012;Sujay et al, 2012;Wang et al, 2013 (Gautami et al, 2012b;Ravi et al, 2011;Varshney et al, 2009b) Gautami et al, 2012a;Liang et al, 2009;Ravi et al, 2011;Selvaraj et al, 2009;Shirasawa et al, 2012;Varshney et al, 2009b) (Continued on next page) (Liang et al, 2009;Pandey et al, 2012c;Sarvamangala et al, 2011;Selvaraj et al, 2009;Shirasawa et al, 2012) Pea Plant height QTL 19-65 (Tullu et al, 2008;Tar'an et al, 2003b;Dirlewanger et al, 1994) (Atibalentja et al, 2005;Guo et al, 2006;Guo et al, 2008;Han et al, 2008;Huynh et al, 2010;Kazi et al, 2008;Ruben et al, 2006;Shi et al, 2008;Silva and Danielle, 2008;Vuong et al, 2008;Wang et al, 2010;Winter et al, 2007) (Continued on next page) Funatsuki et al, 2005;Githiri et al, 2006;Githiri et al, 2007;…”

Disease resistance in pea (Pisum sativum L.) types for autumn sowings in Mediterranean environments - a review

“…Resistance conferred by this gene has been proved to be stable and is caused by a barrier to the pathogen penetration . RFLP, RAPD/SCAR and SSR markers linked to the er1 locus have been identified (Dirlewanger et al 1994;Timmerman-Vaughan et al 1994;Tiwari et al 1998;Ek et al 2005;Pereira et al 2009). …”