Reticulated Vitreous Carbon-Based Composite Bienzyme Electrodes for the Determination of Alcohols in Beer Samples

Amperometric Ethanol Biosensor Based on Integration of Alcohol Dehydrogenase with Meldola's Blue/Ordered Mesoporous Carbon Electrode

“…Figure 7 displays the amperometric response for successive addition of 1 mM ethanol into a stirred 50 mM phosphate buffer (pH 6.85) containing 5 mM NAD þ (A) and the corresponding calibration curve (B) recorded at the ADH/MB/OMC/GCE under the operation potential of À 0.1 V. Apparently, each addition of ethanol gives rise to a quick steep rise in current above the background which results in a steady state response within 7 AE 1 s. The linear response range of the biosensor was up to 6 mM (R ¼ 0.994) with a detection limit of 19.1 AE 0.58 mM (S/N ¼ 3) and a sensitivity of 34.58 AE 2.43 nA/mM. All of them are superior to those of the amperometric ethanol biosensors described elsewhere [3, 33 -35] but not as good as those of alcohol oxidase based biosensors reported in [36,37]. The apparent Michaelis -Menten constant (K app M Þ of the ethanol biosensor was calculated to be 3.15 AE 0.021 mM according to the Lineweaver -Burk equation [38].…”

Application of Enzyme Biosensors in Analysis of Food and Beverages

“…A combination of alcohol oxidase (AOx) together with horseradish peroxidase (HRP) and ferrocene as a mediator incorporated into reticulated vitreous carbon-epoxy resin electrode matrices was described for alcohol measurement (Pena et al 2002). Another way of immobilization this bienzymatic combination is the use of electrodeposition paints (EDPs) with a first layer integrating HRP within an Os-complex modified EDP in order to assure fast electron transfer between the enzyme and the electrode surface.…”

Biosensors - classification, characterization and new trends

“…The most successful commercially available biosensors are those for measuring glucose in blood samples representing about 90 % of the global biosensor market utilizing glucose oxidase or glucose dehydrogenase ( b Monošík et al 2012). Variety of enzymes were used for biosensor construction, for example oxidoreductase enzymes were used for lactate (Huang et al 2009, Huang et al 2008, Katrlík et al 1999, Pereira et al 2007, malate (Arif et al 2002, c Monošík et al 2012, Prodromidis et al 1996, ascorbate , amino acids (Pollegioni et al 2007, Sacchi et al 1998, alcohol (Katrlík et al 1998, Pena et al 2002, Smutok et al 2006, Tkáč et al 2003, cholesterol (Lia and Gub 2006, Umar et al 2009, Vidal et al 2004, glycerol (Alvarez-Gonzalez et al 2000, d Monošík et al 2012, Niculescu et al 2003, fructose (Tkáč et al 2001, Tkáč et al 2002, transferase can be utilized in biosensorical analysis of acetic acid (Mieliauskiene et al 2006, Mizutani et al 2003, determination of xenobiotics such as captan (Choi et al 2003) or atrazine (Andreou and Clonis 2002), hydrolase in sucrose (Soldatkin et al 2008, Surareungchai et al 1999, lyase in citric acid analysis (Maines et al 2000, Prodromidis et al 1997, ligase in DNA point mutation detection (Pang et al 2006), isomerase for 19-norandrostenedione (Sheu et al 2008), etc. Many factors have infl uence on the performance of enzyme-based biosensors, such an enzyme l...…”