Reverse Transcriptase Associated with A-type Particles from Murine Myeloma Cells

Thach, Robert E.; Robertson, DL; Baenziger, Nancy Lewis; Dobbertin, D C

doi:10.1101/sqb.1974.039.01.110

Cited by 4 publications

(3 citation statements)

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“…components; however, in longer sedimentation runs they show up clearly (12). The presence of 18S and 27S species in the A particle RNA suggests the possibility Time course of the hybridization of ['HJDNA reaction product to A particle RNA.…”

Section: Resultsmentioning

confidence: 97%

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Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells

Robertson¹,

Baenziger²,

Dobbertin³

et al. 1975

J Virol

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The RNA-dependent DNA polymerase present in intracisternal A-type particles from mouse myeloma tumor cells has been studied. This polymerase can use either endogenous A particle RNA or an exogenous synthetic polynucleotide [poly(rA) ] as a template. The DNA reaction product is small (4S-1OS) and over 90% of it hybridizes to A particle RNA, whereas up to 50% of it hybridizes to murine sarcoma-leukemia virus RNAs. The RNA isolated from purified A particles is generally of low molecular weight (5S-15S) but contains small amounts of 70S and 35S components. These results suggest that A-type particles may be related to C-type oncornaviruses.

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Section: Resultsmentioning

confidence: 97%

“…While this work was in progress, Yang and Wivel (19) also reported that an endogenous reverse transcriptase activity can be detected in A particle preparations. A preliminary report of some of our data has been presented elsewhere (12).…”

mentioning

confidence: 98%

Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells

Robertson¹,

Baenziger²,

Dobbertin³

et al. 1975

J Virol

View full text Add to dashboard Cite

show abstract

“…Type A particles resemble morphologically the cores of immature types B and C particles (22), but their biochemical similarity to these oncornaviruses has only recently been confirmed. Two research groups have reported that intracisternal A particles contain the characteristic reverse transcriptase and traces of 70S and 35S RNA which are common elements in all oncornaviruses (23,32,38,39). Moreover, when complementary DNA (cDNA) was prepared using the A particle reverse transcriptase, 40 to 50% of this cDNA hybridized to Harvey and Moloney sarcoma virus 70S RNAs (23).…”

mentioning

confidence: 99%