Review : A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation

Capani, Francisco; Saraceno, Ezequiel; Boti, Valeria; Aon-Bertolino, Laura; Fernández, Juan Carlos; Gato, Fernando; Krause, Maria Sol; Giráldez, Lisandro; Ellisman, Mark H.; Coirini, Héctor

doi:10.32604/biocell.2008.32.001

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…High levels of glutamate produce a cascade of events in dendritic spines that lead to cell death [ 25 , 31 , 33 , 43 , 48 , 52 – 55 ]. Since structure and function of dendritic spines are dynamically regulated by different cellular pathways acting on the actin cytoskeleton, we used light and electron microscopic techniques that had previously been used in our laboratory [ 1 , 56 ] and others [ 4 , 8 ] to study F-actin modifications induced by PA. By confocal microscope analyses, we observed dendritic spines represented by punctate staining using Phalloidin-Alexa 568 . PA animals showed a decrease in punctate staining respect to CTL group ( Figure 5 Top ) ( P < 0.01).…”

Section: Resultsmentioning

confidence: 99%

Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia

et al. 2012

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Perinatal asphyxia (PA) affects the synaptic function and morphological organization. In previous works, we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia leading to long-term ubi-protein accumulation. Since F-actin is highly concentrated in dendritic spines, modifications in its organization could be related with alterations induced by hypoxia in the central nervous system (CNS). In the present study, we investigate the effects of PA on the actin cytoskeleton of hippocampal postsynaptic densities (PSD) in 4-month-old rats. PSD showed an increment in their thickness and in the level of ubiquitination. Correlative fluorescence-electron microscopy photooxidation showed a decrease in the number of F-actin-stained spines in hippocampal excitatory synapses subjected to PA. Although Western Blot analysis also showed a slight decrease in β-actin in PSD in PA animals, the difference was not significant. Taken together, this data suggests that long-term actin cytoskeleton might have role in PSD alterations which would be a spread phenomenon induced by PA.

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Section: Resultsmentioning

confidence: 99%

Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia

et al. 2012

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“…S5). To test the hypothesis that exogenous sterols can remodel the actin cytoskeleton, we visualized the filamentous actin (F-actin) configuration in the sporangia of P. capsici with phalloidin staining ( 35 ). The results showed that F-actin was evenly distributed in the sporangia of wild-type P. capsici cultured without sterol while in the presence of β-sitosterol the F-actin aggregated orderly at the zoospore cleavage site during zoosporogenesis ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Sterol-Sensing Domain (SSD)-Containing Proteins in Sterol Auxotrophic Phytophthora capsici Mediate Sterol Signaling and Play a Role in Asexual Reproduction and Pathogenicity

et al. 2023

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“…Dendritic spines are small membrane protrusions whose shapes are supported by a highly dynamic cytoskeleton that consists primarily of filamentous actin ( Fifková and Delay, 1982 ; Matus et al, 1982 ; Capani et al, 2001a , b , c , 2005 , 2008 ; Ouyang et al, 2005 ). Spine shape and size correlate with postsynaptic current flow into the dendritic tree.…”

Section: Introductionmentioning

confidence: 99%

A Uniform and Isotropic Cytoskeletal Tiling Fills Dendritic Spines

Eberhardt

Bushong²,

Phan³

et al. 2022

eNeuro

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Dendritic spines are submicron, subcellular compartments whose shape is defined by actin filaments and associated proteins. Accurately mapping the cytoskeleton is a challenge, given the small size of its components. It remains unclear whether the actin-associated structures analyzed in dendritic spines of neurons in vitro apply to dendritic spines of intact, mature neurons in situ. Here, we combined advanced preparative methods with multitilt serial section electron microscopy (EM) tomography and computational analysis to reveal the full three-dimensional (3D) internal architecture of spines in the intact brains of male mice at nanometer resolution. We compared hippocampal (CA1) pyramidal cells and cerebellar Purkinje cells in terms of the length distribution and connectivity of filaments, their branching-angles and absolute orientations, and the elementary loops formed by the network. Despite differences in shape and size across spines and between spine heads and necks, the internal organization was remarkably similar in both neuron types and largely homogeneous throughout the spine volume. In the tortuous mesh of highly branched and interconnected filaments, branches exhibited no preferred orientation except in the immediate vicinity of the cell membrane. We found that new filaments preferentially split off from the convex side of a bending filament, consistent with the behavior of Arp2/3-mediated branching of actin under mechanical deformation. Based on the quantitative analysis, the spine cytoskeleton is likely subject to considerable mechanical force in situ .

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Review : A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation

Cited by 10 publications

References 42 publications

Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia

Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia

Sterol-Sensing Domain (SSD)-Containing Proteins in Sterol Auxotrophic Phytophthora capsici Mediate Sterol Signaling and Play a Role in Asexual Reproduction and Pathogenicity

A Uniform and Isotropic Cytoskeletal Tiling Fills Dendritic Spines

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