Review on Research Progress and Prospects of Cicada Flower, Isaria cicadae (Ascomycetes)

Li, Chun Ru; Wang, Yu Qin; Cheng, Wen; Chen, Zhu An; Hywel‐Jones, Nigel L.; Li, Zeng Zhi

doi:10.1615/intjmedmushrooms.2021038085

Cited by 12 publications

(4 citation statements)

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“…The artificial cultivation techniques of C. chanhua include liquid fermentation, solid culture, insect body culture, and ecological mulching soil culture ( 22 , 23 ). Among these methods, the insect body part and fruiting body part of C. chanhua cultivated in insect body culture and ecological mulching soil culture can be harvested and have similar appearance characteristics to wild C. chanhua , which make them more popular ( 2 , 24 ).…”

Section: Introductionmentioning

confidence: 99%

Filtration effect of Cordyceps chanhua mycoderm on bacteria and its transport function on nitrogen

Hu,

Zhou,

Mou

et al. 2024

Microbiol Spectr

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Cordyceps chanhua is a kind of entomopathogenic fungus that has a long medicinal history in traditional Chinese medicine. In this study, we aimed to explore the role of C. chanhua mycoderm. Our results showed that C. chanhua mycoderm has a certain ability to transfer nitrogen, which can transfer nitrogen from the insect body to the external environment and transfer nitrogen from the environment to C. chanhua . By studying the filtration of bacterial mycoderm, it was found that the mycoderm of C. chanhua was able to filter out most of the bacteria in the environment. Using the high-throughput sequencing methods, we found that the abundance of bacterial community first increased and then decreased during the growth and development of C. chanhua . The bacterial richness and diversity of mycoderm of C. chanhua cultivated in sterile glass bottles were significantly lower than those cultivated in soil mulching. In addition, this study also found that the mycoderm of C. chanhua formed under both soil mulching cultivation and sterile glass bottle cultivation was hydrophobic. The mycoderm of C. chanhua , which occurred under sterile glass bottle cultivation, was closely and orderly arranged, while the mycoderm of C. chanhua cultivated in soil was quite the opposite. The density and thickness of the C. chanhua membrane in aseptic glass bottle culture were higher than those in soil-covered culture, but the dry/fresh weight was lower than those in soil-covered culture. IMPORTANCE During the natural growth of Cordyceps chanhua , it will form a mycoderm structure specialized from hyphae. We found that the bacterial membrane of C. chanhua not only filters environmental bacteria but also absorbs and transports nitrogen elements inside and outside the body of C. chanhua . These findings are of great significance for understanding the stable mechanism of the internal microbial community maintained by C. chanhua and how C. chanhua maintains its own nutritional balance. In addition, this study also enriched our understanding of the differences in bacterial community composition and related bacterial community functions of C. chanhua at different growth stages, which is of great value for understanding the environmental adaptation mechanism, the element distribution network, and the changing process of symbiotic microbial system after Cordyceps fungi infected the host. At the same time, it can also provide a theoretical basis for some important ecological imitation cultivation technology of Cordyceps fungi.

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Section: Introductionmentioning

confidence: 99%

Filtration effect of Cordyceps chanhua mycoderm on bacteria and its transport function on nitrogen

Hu,

Zhou,

Mou

et al. 2024

Microbiol Spectr

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“…To meet the great consumption demand of cicada flowers, large-scale cultivation of the coremium of C. chanhua has been widely explored and successfully developed in China [4]. A series of commercial products derived from chanhua have been developed and used as functional foods in China [5].…”

Section: Introductionmentioning

confidence: 99%

A Novel Gammapartitivirus That Causes Changes in Fungal Development and Multi-Stress Tolerance to Important Medicinal Fungus Cordyceps chanhua

Zhu

Shi

Wang

et al. 2022

JoF

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Cicada flower, scientifically named Cordyceps chanhua, is an important and well-known Chinese cordycipitoid medicinal mushroom. Although most mycoviruses seem to induce latent infections, some mycoviruses cause host effects. However, the effects of mycovirus on the fungal development and stress tolerance of C. chanhua remain unknown. In this study, we report a novel mycovirus designated Cordyceps chanhua partitivirus 1 (CchPV1) from C. chanhua isolate RCEF5997. The CchPV1 genome comprises dsRNA 1 and dsRNA 2, 1784 and 1563 bp in length, respectively. Phylogenetic analysis using the aa sequences of RdRp revealed that CchPV1 grouped with members of the genus Gammapartitivirus in the family Partitiviridae. We further co-cultivated on PDA donor strain RCEF5997 and recipient C. chanhua strain RCEF5833 (Vf) for 7 days, and we successfully obtained an isogenic line of strain RCEF5833 with CchPV1 (Vi) through single-spore isolation, along with ISSR marker and dsRNA extraction. The biological comparison revealed that CchPV1 infection slows the growth rate of the host, but increases the conidiation and formation of fruiting bodies of the host. Furthermore, the assessment of fungal tolerance demonstrated that CchPV1 weakens the multi-stress tolerance of the host. Thus, CchPV1 infection cause changes in fungal development and multi-stress tolerance of the host C. chanhua. The findings of this study elucidate the effects of gammapartitivirus on host entomogenous fungi and provide a novel strategy for producing high-quality fruiting bodies of C. chanhua.

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“…In recent years, scientists have investigated various biological activities of I. cicadae and identified useful compounds, such as amino acids, cordycepin, polysaccharides, and mannitol, which are similar to compounds in Cordyceps sinensis [3]. Some substances are helpful to human health by regulating the immune system, activating macrophages [4,5], promoting phagocytosis of macrophages [6], improving the immune response of the liver, kidney, spleen, and thymus [7], having antiaging properties [8], protecting renal function; alleviating glomerulosclerosis and improving chronic renal failure [9,10], alleviating renal anemia, decreasing blood glucose [11], having antitumor properties [12], and improving vision [13].…”

Section: Introductionmentioning

confidence: 99%

“…I. cicadae can form a special type of asexual fruiting body referred to as coremia or synnema [4]. Mature synnemata are composed of large aggregations of mostly parallel hyphae to form a yellow-to-brown pigmented bundle of hyphae growing erect from the substrate.…”

Section: Introductionmentioning

confidence: 99%

GPI-Anchored Protein Homolog IcFBR1 Functions Directly in Morphological Development of Isaria cicadae

Liu

Gai

Meng

et al. 2022

JoF

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Isaria cicadae is a famous edible and medicinal fungus in China and Asia. The molecular basis of morphogenesis and synnemal formation needs to be understood in more detail because this is the main source of biomass production in I. cicadae. In the present study, a fruiting body formation-related gene with a glycosylphosphatidylinositol (GPI) anchoring protein (GPI-Ap) gene homolog IcFBR1 was identified by screening random insertion mutants. Targeted deletion of IcFBR1 resulted in abnormal formation of synnemata, impairing aerial hyphae growth and sporulation. The IcFBR1 mutants were defective in the utilization of carbon sources with reduced polysaccharide contents and the regulation of amylase and protease activities. Transcriptome analysis of ΔIcfbr1 showed that IcFBR1 deletion influenced 49 gene ontology terms, including 23 biological processes, 9 molecular functions, and 14 cellular components. IcFBR1 is therefore necessary for regulating synnemal development, secondary metabolism, and nutrient utilization in this important edible and medicinal fungus. This is the first report illustrating that the function of IcFBR1 is associated with the synnemata in I. cicadae.

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Review on Research Progress and Prospects of Cicada Flower, Isaria cicadae (Ascomycetes)

Cited by 12 publications

References 0 publications

Filtration effect of Cordyceps chanhua mycoderm on bacteria and its transport function on nitrogen

Filtration effect of Cordyceps chanhua mycoderm on bacteria and its transport function on nitrogen

A Novel Gammapartitivirus That Causes Changes in Fungal Development and Multi-Stress Tolerance to Important Medicinal Fungus Cordyceps chanhua

GPI-Anchored Protein Homolog IcFBR1 Functions Directly in Morphological Development of Isaria cicadae

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