Rhodohalobacter mucosus sp. nov., isolated from a marine solar saltern

Wang, Tao-Jie; Liu, Zhenwei; Feng, Xi; Zou, Qi-Hang; Du, Zong‐Jun

doi:10.1007/s00203-021-02254-1

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…Both of them have cdsA (encoding phosphatidate cytidylyltransferase), pssA (encoding CDP-diacylglycerol-serine O-phosphatidyltransferase) and psd (encoding phosphatidylserine decarboxylase) genes. This is in accordance with the result of the polar lipid experiment showing that phosphatidylethanolamine is a major polar lipid [44]. In addition, the annotation results of the two genomes indicated that strain C281 T harbours the betB gene related to betainealdehyde dehydrogenase and the betT gene related to choline and betaine uptake as well as betaine biosynthesis system.…”

Section: Phylogenetic Characteristics and Genomic Featuressupporting

confidence: 88%

Membranihabitans maritimus sp. nov., a marine bacterium isolated from deep seawater

Guo

Wang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated C281T, was isolated from seawater sampled at the Marshallese seamount chain. Results of 16S rRNA gene analysis revealed that strain C281T was most closely related to Membranihabitans marinus CZ-AZ5T with 92.7 % sequence similarity. Phylogenetic analysis indicated that the new isolate represented a novel species by forming a distinctive lineage within the family Saprospiraceae . The DNA G+C content of strain C281T was 38.4 mol%. The genome sizes of strain C281T and the reference strain M. marinus CZ-AZ5T were 5 962 917 and 5 395 999 bp, respectively. The average nucleotide identity and in silico DNA–DNA hybridization values between strains C281T and M. marinus CZ-AZ5T were found to be low (69.3 and 17.6 %, respectively). Different functional genes were found in the genome of strain C281T, such as CZC CBA, polysaccharide utilization loci and linear azol(in)e-containing peptide cluster coding genes. The NaCl range for growth was 0.5–15.0 %. Positive results were obtained for hydrolysis of Tween 60 and urease. MK-7 was the sole respiratory quinone. The major fatty acids were C16 : 1 ω6c and/or C16 : 1 ω7c, iso-C15 : 0 and iso-C15 : 1 F. The major polar lipids of strain C281T were phosphatidylethanolamine, phosphatidylglycerol, two unidentified lipids and five unidentified glycolipids. On the basis of its taxonomic characteristics, the isolate represents a novel species of the genus Membranihabitans, for which the name Membranihabitans maritimus sp. nov. (type strain C281T=KCTC 92171T=MCCC M27001T) is proposed.

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Section: Phylogenetic Characteristics and Genomic Featuressupporting

confidence: 88%

Membranihabitans maritimus sp. nov., a marine bacterium isolated from deep seawater

Guo

Wang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…In addition to the osmotic stress response mechanism, strain YR1-1 T contained 12 genes for sulphur metabolism by KEGG ortholog-based potential metabolic pathways (Table S3). A recent research revealed that members of Balneolaceae have the integrated arginine and proline metabolism pathway [7]. Similarly, strain YR4-1 T had genes encoding the relevant enzymes (listed in Table S3).…”

Section: Genome Featuresmentioning

confidence: 99%

“…Balneolaceae strains have been isolated from various saline environments, e.g. marine solar saltern [1,7], seawater [2,8,9], a Synechococcus culture [3], salt mine [4,5,10], saltern [6,11,12], deep-sea sediment [13], saline lake [14] and saline-alkali wetland soil [15]. At present, there are only a few studies that describe the characteristics of the family Balneolaceae, and little is known about its ecological features.…”

Section: Introductionmentioning

confidence: 99%

Halalkalibacterium roseum gen. nov., sp. nov., a new member of the family Balneolaceae isolated from soil

Wang

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, non-motile, moderately halophilic and facultatively anaerobic bacterium, designated YR4-1T, was isolated from a saline-alkali and sorghum-planting soil sample collected in Dongying, Shandong Province, PR China. Growth occurred at 28–45 °C with the presence of 4.0–20.0 % (w/v) NaCl and pH 6.0–9.0. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that YR4-1T shared the highest similarity of 92.1–92.4 % with the valid published species of Aliifodinibius . The isolate formed a separate clade at the genus level in recently described family Balneolaceae . The draft genome of strain YR4-1T is 3.83 Mbp long with 44.0 mol% G+C content. The strain possesses several genes involved in the osmotic stress response mechanism and diverse metabolic pathways, probably for the living in saline environment. This may lead to a better understanding of the underrepresented Balneolaceae lineage. The major menaquinone was MK-7. The main polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids, aminophosphoglycolipid, one glycolipid, and four unidentified lipids. The predominant cellular fatty acids were iso-C15 : 0 (35.7 %) and anteiso-C15 : 0 (33.5 %). On the basis of its phenotypic, chemotaxonomic and phylogenetic features, strain YR4-1T represents a novel species of a new genus, for which the name Halalkalibacterium roseum gen. nov., sp. nov. is proposed. The type strain is YR4-1T (=CGMCC 1.17777T=KCTC 72795T).

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“…The genus Rhodohalobacter, belonging to the family Balneolaceae (Xia et al 2016), was rstly established by Xia et al (2017). At the time of writing, there are two valid species: Rhodohalobacter halophilus (Xia et al 2017) and Rhodohalobacter barkolensis (Han et al 2018), and one unvalidated species: 'Rhodohalobacter mucosus' (Wang et al 2021), according to the List of Prokaryotic names with Standing in Nomenclature (LPSN) (https://lpsn.dsmz.de). All above were isolated from saline environments, including salterns and saline lakes.…”

Section: Introductionmentioning

confidence: 99%

Rhodohalobacter sulfatireducens sp. nov., isolated from a marine solar saltern

et al. 2022

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A novel Gram-stain-negative, oxidase-positive, catalase-positive, non-motile, facultatively anaerobic, and rod-shaped bacterium, designated WB101 T , was isolated from a marine solar saltern located in Wendeng, PR China. Growth occurs at 25-42°C (optimum, 35-37°C), 1.0-11.0% (w/v) NaCl (optimum, 5.0-6.0%), and pH 7. 0-8.5 (optimum, 7.5-8.0). Analysis based on the 16S rRNA gene sequences revealed that WB101 T shared high level of sequence similarity with Rhodohalobacter barkolensis 15182 T (93.5%), Rhodohalobacter halophilus JZ3C29 T (93.2%), and 'Rhodohalobacter mucosus' 8A47 T (92.1%). Strain WB101 T formed an evident species-level clade within the genus Rhodohalobacter in both phylogenetic and phylogenomic topologies. The draft genome of strain WB101 T is 5,104,032 bp in size, and the G + C content is 42.0 mol%. The genomic sequencing yields 138 contigs with an N50 value of 123,641 and a coverage depth of 275.0 ×. The strain encodes a complete assimilatory sulfate reduction pathway. The sole respiratory quinone was menaquinone 7. The dominant cellular fatty acid (≥ 10%) was iso-C 15:0 . The polar lipid pro le of the novel isolate included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine. Based on its phylogenetic, physiological, and biochemical properties, strain WB101 T should be classi ed as a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter sulfatireducens sp. nov. is proposed. The type strain is WB101 T (= KCTC 92204 T = MCCC 1H00518 T ).

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Rhodohalobacter mucosus sp. nov., isolated from a marine solar saltern

Cited by 10 publications

References 16 publications

Membranihabitans maritimus sp. nov., a marine bacterium isolated from deep seawater

Membranihabitans maritimus sp. nov., a marine bacterium isolated from deep seawater

Halalkalibacterium roseum gen. nov., sp. nov., a new member of the family Balneolaceae isolated from soil

Rhodohalobacter sulfatireducens sp. nov., isolated from a marine solar saltern

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