Role of ADF/TRX and Its Inhibitor on the Release of Major Basic Protein from Human Eosinophils

Koyanagi, H; Wakasugi, Naomi; Yoshimatsu, Kentaro; Takashima, Yuichiro; Yodoi, Junji; Momoi, Mariko Y.; Suda, Toshio; Kimura, Tadashi; Yamaguchi, Yuji

doi:10.1006/bbrc.1995.2245

Cited by 6 publications

(4 citation statements)

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“…Thioredoxin reductase is an essential enzyme important in maintaining cellular thiol-redox homeostasis and defense against oxidant stress and also mediates important cocytokine activities via its substrate thioredoxin, which can act as a potent T-cell growth factor (21)(22)(23)(24)(25)(26)(27)(28)(29)(30). Increases in thioredoxin reductase may thus not only signal HDI-induced thiolredox imbalance but also provide an "antigenreceptor-independent" mechanism by which epithelial cell responses to exposure may promote immunologic sensitization to HDI via either a) epithelial cell-derived cytokines/ chemokines under redox-dependent transcriptional regulation (13,14) or b) immunologically active thioredoxin (21)(22)(23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

“…Increases in thioredoxin reductase may thus not only signal HDI-induced thiolredox imbalance but also provide an "antigenreceptor-independent" mechanism by which epithelial cell responses to exposure may promote immunologic sensitization to HDI via either a) epithelial cell-derived cytokines/ chemokines under redox-dependent transcriptional regulation (13,14) or b) immunologically active thioredoxin (21)(22)(23)(24)(25). Interestingly, thioredoxin reductase contains selenium, which is thought to be crucial to its role as a cellular sensor of redox potential and which, based on its biochemical properties, should be highly susceptible to nucleophilic addition reactions with diisocyanates (10,28,31).…”

Section: Discussionmentioning

confidence: 99%

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Effects of hexamethylene diisocyanate exposure on human airway epithelial cells: in vitro cellular and molecular studies.

Wisnewski

Liu

Miller

et al. 2002

Environ Health Perspect

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In this study we developed an in vitro exposure model to investigate the effects of hexamethylene diisocyanate (HDI) on human airway epithelial cells at the cellular and molecular level. We used immunofluorescence analysis (IFA) to visualize the binding and uptake of HDI by airway epithelial cell lines (A549 and NCI-NCI-H292) and microarray technology to identify HDI sensitive genes. By IFA, we observed that subcytotoxic concentrations of HDI form microscopic micelles that appear to be taken up by cells over a 3-hr period postexposure. Microarray analysis (4.6K genes) of parallel cultures identified four genes (thioredoxin reductase, dihydrodiol dehydrogenase, TG interacting factor, and stanniocalcin) whose mRNA levels were up-regulated after HDI exposure. Northern analysis was used to confirm that HDI increased message levels of these four genes and to further explore the dose dependence and kinetics of the response. The finding that HDI exposure increases thioredoxin reductase expression supports previous studies suggesting that HDI alters thiol-redox homeostasis, an important sensor of cellular stress. Another of the HDI-increased genes, a dihydrodiol dehydrogenase, encodes a protein previously shown to be specifically susceptible to HDI conjugation, and known to detoxify other hydrocarbons. Together, the data describe a novel approach for investigating the effects of HDI binding and uptake by human airway epithelial cells and begin to identify genes that may be involved in the acute response to exposure.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of hexamethylene diisocyanate exposure on human airway epithelial cells: in vitro cellular and molecular studies.

Wisnewski

Liu

Miller

et al. 2002

Environ Health Perspect

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“…5) It has been reported to inhibit the thioredoxin (TRX) system and to exhibit cytotoxicity against several human tumor cell lines. [5][6][7] In addition, strong repression of human immunodeficiency virus (HIV) virus replication was also reported for this compound. 7) In spite of its interesting biological activities, the absolute configurations of (Ϫ)-2 have not been determined, while racemic synthesis of an epimer (rac-3) at C(6a) of 2 was reported.…”

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confidence: 85%

First Syntheses of (-)-Tauranin and Antibiotic (-)-BE-40644 Based on Lipase-Catalyzed Optical Resolution of Albicanol

Ishii

Fujii

Akita

2009

CHEMICAL & PHARMACEUTICAL BULLETIN

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First syntheses of sesquiterpene quinones (؊ ؊)-tauranin and (؊ ؊)-BE-40644 which exhibited strong cytotoxicity against several cancer cell lines, were achieved from (8aS)-albicanol obtained by enzymatic optical resolution. By comparison of the sign of specific rotation between synthetic (12bS)-BE-40644 and natural (؊ ؊)-BE-40644, the absolute configurations of natural (؊ ؊)-BE-40644 were determined to be 4aS, 6aS, 12aR, 12bS.

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“…32 The YJ cells were washed twice with HBSS/2ME and resuspended in the same medium at 5 × 10 4 cell/ml. Aliquots of cell suspensions of 0.2 ml were added to the Eppendorf tubes.…”

Section: Assay Of Mbp Concentrationmentioning

confidence: 99%

Models of lineage switching in hematopoietic development: a new myeloid-committed eosinophil cell line (YJ) demonstrates trilineage potential

et al. 1998

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Role of ADF/TRX and Its Inhibitor on the Release of Major Basic Protein from Human Eosinophils

Cited by 6 publications

References 0 publications

Effects of hexamethylene diisocyanate exposure on human airway epithelial cells: in vitro cellular and molecular studies.

Effects of hexamethylene diisocyanate exposure on human airway epithelial cells: in vitro cellular and molecular studies.

First Syntheses of (-)-Tauranin and Antibiotic (-)-BE-40644 Based on Lipase-Catalyzed Optical Resolution of Albicanol

Models of lineage switching in hematopoietic development: a new myeloid-committed eosinophil cell line (YJ) demonstrates trilineage potential

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