Role of bacteria in the adsorption and binding of DNA on soil colloids and minerals

Cai, Peng; Zhu, Jun; Huang, Qiaoyun; Fang, Linchun; Liang, Wei; Chen, Wenli

doi:10.1016/j.colsurfb.2008.10.008

Cited by 21 publications

(17 citation statements)

References 31 publications

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“…where Q e represents the extent of adsorption of DNA on the adsorbent (mg of DNA g -1 of absorbent); Q m is the maximum adsorption (mg g -1 ); b, K f , and n are constants; C e is the equilibrium concentration of DNA (mg L -1 ) [8,14]. The adsorption parameters obtained from the two models are listed in Table 1.…”

Section: Effect Of Adsorbentmentioning

confidence: 99%

“…Washing was repeated until no DNA was detected by UV in the supernatant. The percentage of DNA desorption (%) = the total amount of DNA desorbed/the amount of DNA adsorbed [14].…”

Section: Synthesis Of Clay/dna Hybridsmentioning

confidence: 99%

“…The solution pH was adjusted by HCl and NaOH solutions. After the adsorption for 2 h, the suspension was centrifuged at 10,000 rpm for 20 min, and the resultant solids were filtered and dried in a vacuum oven at 60°C for 12 h [14]. The obtained samples were referred to clay/DNA hybrids.…”

Section: Synthesis Of Clay/dna Hybridsmentioning

confidence: 99%

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Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Hou

Huang

et al. 2014

J Mater Sci

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Montmorillonite (MMT) and rectorite (REC) are clay minerals that consist of hydrated aluminum silicate with Si-O tetrahedrons on the bottom of the layer, Al-O(OH) 2 octahedrons on the top, and various exchangeable ions such as Na ? and Ca 2? in interlayer. However, the driving force for intercalation of double-stranded DNA is not sufficient to open up the gallery of MMT and REC and allows the intercalation to occur. Furthermore, the external surface of MMT and REC is hydrophilic rather than organophilic. In the study, cationic hexadecyl trimethyl ammonium bromide (CTAB) is chosen to modify MMT and REC to make clay minerals more compatible with DNA. CTAB intercalates into the galleries of clay minerals and expands the basal spacing for DNA intercalation. Novel CTAB-clay/DNA hybrids are synthesized for the first time, with their structure investigated by X-ray diffraction and Fourier transform infrared. Gel electrophoresis analysis confirms that the CTAB-modified clay could protect DNA from degradation of DNase I. UV absorption spectroscopy and circular dichroism indicate that the modified clay minerals can provide a capacity for protecting DNA from damage induced by heavy metals. In addition, the intercalated DNA can be recovered readily under alkaline conditions. Therefore, CTAB-clay/DNA hybrids are potential materials for storage of genetic information.

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Section: Effect Of Adsorbentmentioning

confidence: 99%

“…Washing was repeated until no DNA was detected by UV in the supernatant. The percentage of DNA desorption (%) = the total amount of DNA desorbed/the amount of DNA adsorbed [14].…”

Section: Synthesis Of Clay/dna Hybridsmentioning

confidence: 99%

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Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Hou

Huang

et al. 2014

J Mater Sci

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“…"Free" or "extracellular" DNA are typically released during cell growth (Lorenz and Wackernagel, 1994;Lotareva and Prozorov, 2000), from a biofilm or cell remnants (Cai et al, 2009;Lorenz and Wackernagel, 1994) or through lysis of dead bacteria (Lorenz and Wackernagel, 1994). However, DNA released into the aqueous environment or soil is subjected to degradation by dynamic biological, physical, and chemical factors (Levy-Booth et al, 2007) which are working against the success of HGT.…”

Section: Introductionmentioning

confidence: 99%

Mineral Facilitated Horizontal Gene Transfer: A New Principle for Evolution of Life?

Sand

Jelavić

2017

Preprint

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A number of studies have highlighted that adsorption to minerals increases DNA longevity in the environment. Such DNA-mineral associations can essentially serve as pools of genes that can be stored across time. Importantly, this DNA is available for incorporation into alien organisms through the process of horizontal gene transfer. Here we argue that minerals hold an unrecognized potential for successfully transferring genetic material across environments and timescales to distant organisms and hypothesize that this process has significantly influenced the evolution of life. Our hypothesis is illustrated in the context of the evolution of early microbial life and the oxygenation of the Earth’s atmosphere and offers an explanation for observed outbursts of evolutionary events caused by horizontal gene transfer.

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“…However, using single natural polymers as a drug carrier may cause the instability of drug owing to its water‐solubility and degradability 3, 4. Clay minerals, include smectites, palygorskite, kaolinite and talc, have a high specific area and absorptive capacity, rheological properties and low or null toxicity and are usually used in Pharmaceutical Technology and Dermopharmacy as ideal excipients and substances of suitable biological activity 5, 6. In addition, clays are also used to delay and/or target drug release or even improve drug dissolution and simultaneously increase drug stability 7–9.…”

Section: Introductionmentioning

confidence: 99%

Effect of attapulgite contents on release behaviors of a pH sensitive carboxymethyl cellulose‐g‐poly(acrylic acid)/attapulgite/sodium alginate composite hydrogel bead containing diclofenac

Wang

2011

J of Applied Polymer Sci

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A series of pH-sensitive composite hydrogel beads, carboxymethyl cellulose-g-poly(acrylic acid)/attapulgite/sodium alginate (CMC-g-PAA/APT/SA), were prepared by combining CMC-g-PAA/APT composite and SA, using Ca 2þ as the ionic crosslinking agent and diclofenac sodium (DS) as the model drug. The effects of APT content and external pH on the swelling properties and release behaviors of DS from the composite hydrogel beads were investigated. The results showed that the composite hydrogel beads exhibited good pH-sensitivity. Introducing 20% APT into CMC-g-PAA hydrogel could change the surface structure of the composite hydrogel beads, decrease the swelling ability, and relieve the burst release effect of DS. The drug cumulative release ratio of DS from the hydrogel beads in simulated gastric fluid was only 3.71% within 3 hour, but in simulated intestinal fluid about 50% for 3 hour, 85% for 12 hour, up to 90% after 24 hour. The obtained results indicated that the CMC-g-PAA/APT/SA hydrogel beads could be applied to the drug delivery system as drug carriers in the intestinal tract.

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Role of bacteria in the adsorption and binding of DNA on soil colloids and minerals

Cited by 21 publications

References 31 publications

Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Mineral Facilitated Horizontal Gene Transfer: A New Principle for Evolution of Life?

Effect of attapulgite contents on release behaviors of a pH sensitive carboxymethyl cellulose‐g‐poly(acrylic acid)/attapulgite/sodium alginate composite hydrogel bead containing diclofenac

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