Role of recombinant human erythropoietin loading chitosan-tripolyphosphate nanoparticles in busulfan-induced genotoxicity: Analysis of DNA fragmentation via comet assay in cultured HepG2 cells

Ghassemi-Barghi, Nasrin; Varshosaz, Jaleh; Etebari, Mahmoud; Jafarian-Dehkordi, Abbas

doi:10.1016/j.tiv.2016.07.001

Cited by 28 publications

(36 citation statements)

References 42 publications

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“…Slides were evaluated using Comet Score software and 100 randomly selected nucleoids (50 nucleoids from each replicate slide) were analyzed per treatment. Three independent experiments were performed [38].…”

Section: Single-cell Gel Electrophoresis (The Comet Assay)mentioning

confidence: 99%

“…According to the results, the size of the nanoparticles was ~110 nm, zeta potential was 45 mV, poly disparity index was 0.28, and the melatonin EE was 75% (100 µmol/L). The release profile of melatonin nanoparticles demonstrated a biphasic release: an early burst release phase and a slow release phase [38] (Fig. 1).…”

Section: Characterization Of Melatonin Nanoparticlesmentioning

confidence: 99%

“…Single-cell gel electrophoresis (comet assay) is considered a sensitive method for analyzing genotoxic or genoprotective potential of compounds and is commonly used in genotoxicity testing [37,38]. The purpose of the present study was to explore the protective effect of melatonin nanoparticles against etoposide-induced genotoxicity.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin Loading Chitosan-Tripolyphosphate Nanoparticles: Application in Attenuating Etoposide-Induced Genotoxicity in HepG2 Cells

Shokrzadeh

Ghassemi-Barghi²

2018

Pharmacology

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Etoposide is one of the most effective chemotherapeutic agents used in the treatment of various types of cancers. However, as a Topoisomerase II inhibitor, during clinical use, several side effects may occur. In addition, in several in vivo and in vitro studies, etoposide has been shown to have a range of genotoxic effects including single and double strand breaks. Melatonin is an anti-aging and antioxidant hormone synthesized from the pineal gland. The genoprotective, antioxidant, and free radical scavenger properties of melatonin have been well explained in various studies. The aim of this study was to explore whether melatonin nanoparticles protects against etoposide-induced genotoxicity in the HepG2 cell line. HepG2 cells (25 × 10⁴ cells/well) were cultured in 24-well plates: a control group and 3 melatonin and its nanoparticles + etoposide groups (pre- and cotreatment conditions). Our results show that etoposide induced a noticeable genotoxic effect in HepG2 cells. Melatonin reduced the effects of etoposide significantly in both types of experiment conditions, through the reduction of the level of DNA damage measured via comet assay. Furthermore, melatonin decreased the intracellular reactive oxygen species generation. It also increased the intracellular glutathione levels in HepG2 cells. Nano melatonin is more effective than regular melatonin. The most protective effect was observed with melatonin when it was administrated 24 h before etoposide treatment.

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Section: Single-cell Gel Electrophoresis (The Comet Assay)mentioning

confidence: 99%

Section: Characterization Of Melatonin Nanoparticlesmentioning

confidence: 99%

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Melatonin Loading Chitosan-Tripolyphosphate Nanoparticles: Application in Attenuating Etoposide-Induced Genotoxicity in HepG2 Cells

Shokrzadeh

Ghassemi-Barghi²

2018

Pharmacology

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“…The most commonly used parameters in the comet assay method are tail moment, tail length, and percent of DNA in tail (27,28). All these parameters were used in the present study to evaluate DNA damage.…”

Section: Introductionmentioning

confidence: 99%

Modulatory Effects of Dexrazoxane Against Genotoxicity and Lipid Peroxidation Induced by Idarubicin in HepG2 Cells

2018

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Background: Idarubicin is an anthracycline antibiotic drug widely used in chemotherapy. Dexrazoxane is an iron chelator used clinically against anthracyclines-induced cardiotoxicity. The present study was designed to determine the possible genoprotection of dexrazoxane on idarubicin-induced DNA damage and oxidative stress. Methods: In this study, the induction of DNA damage by idarubicin was examined on HepG2 cells, using comet assay. Cells were exposed to different concentrations of idarubicin in order to find the minimum and suitable genotoxic concentration. To survey the genoprotective effects of dexrazoxane, cells were subjected to several safe concentrations of dexrazoxane (10, 50, 100, and 200

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“…Single cell gel electrophoresis (comet assay) is considered as some sensitive methods for analyzing genotoxic or genoprotective potential of compounds is normally used in genotoxicity testing. Applications of this test include genotoxicity testing, human biomonitoring and molecular epidemiology, ecogenotoxicology, as well as primary research in DNA damage and repair [27,28]. The purpose of present study was to explore the protective effect amifostine against irinotecan induced genotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Genoprotective Effects of Amifostine against Irinotecan Toxicity in Human Hepatoma Cells

2018

IJCRT

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Irinotecan (CPT-11) is a topoisomerase inhibitor anticancer drug effective against many human malignancies. Several mechanisms have been proposed for the antitumor effects of irinotecan, such as DNA synthesis inhibition, DNA crosslinking, inhibition of topoisomerase I, free radical generation and lipid peroxidation. Amifostine, is a cytoprotective adjuvant used in cancer chemotherapy, involving DNA-binding chemotherapeutic agents. The aim of this study was to explore whether amifostine protects against irinotecan-induced genotoxicity in HepG2 cells. For this purpose, we measured the DNA damage level with comet assay in HepG2 cells treated with irinotecan and amifostine in different condition. We also measured the intracellular ROS generation and GSH levels in cells treated with irinotecan and amifostine in pre-treatment condition. Our results showed that irinotecan induced a noticeable genotoxic effect in HepG2 cells. Amifostine reduced the effects of irinotecan significantly (p<0.0001) by reduction of the level of DNA damage via blocking ROS generation, and enhancement of intracellular glutathione levels.

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Role of recombinant human erythropoietin loading chitosan-tripolyphosphate nanoparticles in busulfan-induced genotoxicity: Analysis of DNA fragmentation via comet assay in cultured HepG2 cells

Cited by 28 publications

References 42 publications

Melatonin Loading Chitosan-Tripolyphosphate Nanoparticles: Application in Attenuating Etoposide-Induced Genotoxicity in HepG2 Cells

Melatonin Loading Chitosan-Tripolyphosphate Nanoparticles: Application in Attenuating Etoposide-Induced Genotoxicity in HepG2 Cells

Modulatory Effects of Dexrazoxane Against Genotoxicity and Lipid Peroxidation Induced by Idarubicin in HepG2 Cells

Antioxidant and Genoprotective Effects of Amifostine against Irinotecan Toxicity in Human Hepatoma Cells

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