Roles of Saccharomyces cerevisiae RAD17 and CHK1 checkpoint genes in the repair of double-strand breaks in cycling cells

Bracesco, Nélson; Candreva, Ema C.; Keszenman, Deborah J.; Sánchez, Ana González; Soria, Sandra; Dell, Mercedes; Siede, Wolfram; Nunes, Elia

doi:10.1007/s00411-007-0119-y

Cited by 5 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, most cells maintain viability without added nutrients and contain an unreplicated set of DNA, just like in the G1 phase [8]. A comparison between the present observations regarding Rad17 and Hdf1 functions in stationary phase and these corresponding to the exponential phase of growth obtained previously [9] may provide some clues to the regulatory networks involved.…”

Section: Introductionsupporting

confidence: 67%

“…Diploid S. cerevisiae mutant strain rad17 /rad17 (WS9156), the corresponding isogenic wild type WS9154 [4,9], and haploid strains SX46A and corresponding isogenic SX46Ahdf1 [7,10] were used in the present study. The WS9154 diploid strain was constructed by mating the haploid SX46A (mating type a) and the haploid WS8105-C (of opposite mating type).…”

Section: Yeast Strains and Culture Conditionsmentioning

confidence: 99%

“…Thereafter, cells were incubated in PBS at 30 • C (liquid holding: LH, 0 ≤ t ≤ 24 h). Either immediately after irradiation (IR) or after different times after incubation in PBS, aliquots of the cell suspensions and respective unirradiated controls were removed for DNA chromosomal analysis and for surviving fraction determinations in YPDA [9,13].…”

Section: Treatmentsmentioning

confidence: 99%

“…Chromosome separation was carried out by transverse alternating field electrophoresis (TAFE; Gene Line II, Beckman, Fullerton, CA). Analysis of chromosomal DNA bands was performed by laser densitometry of the gel photograph negatives (Software: Gel Scan XL Pharmacia) [9,12,13]. Each photograph shows the chromosomal bands corresponding to each cell sample (5 × 10 8 cells/ml).…”

Section: Chromosomal Dna Analysismentioning

confidence: 99%

“…Quantification is possible after laser absorbance analysis [14]. Estimation of mean values of induced DNA double-strand breaks (DBSs) and of corresponding standard deviations were based on the Poisson distribution as: −L Ix/Io, where Ix corresponds to the integral (surface) of a given chromosomal band in the densitograms after treatment and Io is the integral of the same chromosomal band in the respective control sample [9,[13][14][15][16].…”

Section: Chromosomal Dna Analysismentioning

confidence: 99%

See 4 more Smart Citations

HDF1 and RAD17 Genes are Involved in DNA Double-strand Break Repair in Stationary Phase Saccharomyces cerevisiae

et al. 2008

Self Cite

View full text Add to dashboard Cite

DNA repair, checkpoint pathways and protection mechanisms against different types of perturbations are critical factors for the prevention of genomic instability. The aim of the present work was to analyze the roles of RAD17 and HDF1 gene products during the late stationary phase, in haploid and diploid yeast cells upon gamma irradiation. The checkpoint protein, Rad17, is a component of a PCNA-like complex-the Rad17/Mec3/Ddc1 clamp-acting as a damage sensor; this protein is also involved in double-strand break (DBS) repair in cycling cells. The HDF1 gene product is a key component of the non-homologous end-joining pathway (NHEJ). Diploid and haploid rad17 /rad17 , and hdf1 Saccharomyces cerevisiae mutant strains and corresponding isogenic wild types were used in the present study. Yeast cells were grown in standard liquid nutrient medium, and maintained at 30 • C for 21 days in the stationary phase, without added nutrients. Cell samples were irradiated with 60 Co γ rays at 5 Gy/s, 50 Gy ≤ Dabs ≤ 200 Gy. Thereafter, cells were incubated in PBS (liquid holding: LH, 0 ≤ t ≤ 24 h). DNA chromosomal analysis (by pulsed-field electrophoresis), and surviving fractions were determined as a function of absorbed doses, either immediately after irradiation or after LH. Our results demonstrated that the proteins Rad17, as well as Hdf1, play essential roles in DBS repair and survival after gamma irradiation in the late stationary phase and upon nutrient stress (LH after irradiation). In haploid cells, the main pathway is NHEJ. In the diploid state, the induction of LH recovery requires the function of Rad17. Results are compatible with the action of a network of DBS repair pathways expressed upon different ploidies, and different magnitudes of DNA damage.

show abstract

Section: Introductionsupporting

confidence: 67%

Section: Yeast Strains and Culture Conditionsmentioning

confidence: 99%

Section: Treatmentsmentioning

confidence: 99%

Section: Chromosomal Dna Analysismentioning

confidence: 99%

Section: Chromosomal Dna Analysismentioning

confidence: 99%

See 3 more Smart Citations

HDF1 and RAD17 Genes are Involved in DNA Double-strand Break Repair in Stationary Phase Saccharomyces cerevisiae

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

Current awareness on yeast

2008

Yeast

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 5th. Mar. 2008)

show abstract

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin

Bracesco

Sosa

Blanc

et al. 2018

Braz J Med Biol Res

View full text Add to dashboard Cite

DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip.

show abstract

Roles of Saccharomyces cerevisiae RAD17 and CHK1 checkpoint genes in the repair of double-strand breaks in cycling cells

Cited by 5 publications

References 28 publications

HDF1 and RAD17 Genes are Involved in DNA Double-strand Break Repair in Stationary Phase Saccharomyces cerevisiae

HDF1 and RAD17 Genes are Involved in DNA Double-strand Break Repair in Stationary Phase Saccharomyces cerevisiae

Current awareness on yeast

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin

Contact Info

Product

Resources

About