Ruggedness and robustness of conversion factors in method of simultaneous determination of multi-components with single reference standard

Hou, Jinjun; Wu, Wanying; Da, Juan; Shuai, Yao; Long, Huali; Yang, Zhou; Cai, Lei; Yang, Min; Li, Xuan; Jiang, Baohong; Guo, De‐An

doi:10.1016/j.chroma.2011.06.058

Cited by 110 publications

(89 citation statements)

References 8 publications

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“…RFi of the analytes was the ratio of the responses in a unit concentration between chlorogenic acid (As/Cs) and the analyte (Ai/Ci), where A and C were the peak area and the concentration obtained from the standard solution. 30 RRTi was calculated as the ratio of the retention time of the analyte (ti) and chlorogenic acid (tS).…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Method for Quality Evaluation of Houttuyniae Herba by a Single Standard to Determine Multi-components, Fingerprint and HPTLC Method

Dai

et al. 2015

ANAL. SCI.

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A method of quality evaluation by a single standard to determine multi-components (SSDMC), fingerprint and highperformance thin layer-chromatography (HPTLC) was developed for traditional Chinese medicine (TCM), and validated with dry Houttuyniae Herba (HH). In quantitative analysis, an SSDMC method involving nine components has been established with the desirable linearity (r 2 ≥ 0.9998), precision (RSD < 2.7%), accuracy (97.4 -103.1%) and ruggedness. Compared with the results obtained using the external standard method (ESM), this alternative SSDMC method was found to have no statistically significant differences. In fingerprint analysis, nine of fourteen peaks were identified. Simultaneously, 15 HH samples from different origins were classified by similarity analysis and hierarchical clustering analysis (HCA). Additionally, the HPTLC method with three flavonoid markers was firstly established. The combination of SSDMC with the fingerprint and HPTLC method has been verified in the quality control of HH both quantitatively and qualitatively, and will provide a new quality evaluation pattern for TCMs.

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Section: Discussionmentioning

confidence: 99%

A Comprehensive Method for Quality Evaluation of Houttuyniae Herba by a Single Standard to Determine Multi-components, Fingerprint and HPTLC Method

Dai

et al. 2015

ANAL. SCI.

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“…This was consistent with the literature. 26 It can be concluded that the six operational parameters can be adjusted in a narrow range to acquire satisfactory system suitability except for the factor of wavelength.…”

Section: Robustnessmentioning

confidence: 99%

“…[22][23][24] It can reduce the cost and difficulty of simultaneous determination of multiple components, especially when the component is of low content level or unstable and hard to be purified from the plant. 25,26 In this study, high performance liquid chromatography-diode array detection (HPLC-DAD) fingerprint of SR was constructed based on the chromatogram of 15 batches of samples. Twelve components were identified by high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) and related references.…”

Section: Introductionmentioning

confidence: 99%

Fingerprint Analysis and Multi-ingredient Determination Using a Single Reference Standard for Saposhnikoviae Radix

Liao

Liu

et al. 2014

ANAL. SCI.

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A method employing high performance liquid chromatography-diode array detection (HPLC-DAD) has been developed to evaluate the quality of Saposhnikoviae Radix (SR) comprehensively through fingerprint analysis and multi-ingredient determination. In the fingerprint analysis, 12 out of 15 common peaks were identified with high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) and related references. To discriminate the 15 batches of samples collected from different origins, similarity analysis and principal component analysis (PCA) were performed. Meanwhile, the simultaneous quantification of four chromones was achieved by firstly applying a method of using a single standard to determine multiple components (SSDMC) with conversion factor. The method has the advantage of feasibility, economy and simplicity compared with traditional external standard method (ESM). The method possessed desirable linearity (R 2 ≥ 0.9997), precision (RSD <4.0%), accuracy (97.4 -106.6%) and robustness. This study indicated that the method of fingerprint analysis integrated with multi-ingredient determination using the HPLC technique was reliable for the overall quality evaluation of SR.

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“…So far, QAMS has been applied in the quality assessment of Danshen and Sanqi raw herbs and some preparations composed of the two herbs. In Danshen, five phenolic acids [20] and four diterpenoids [21,25] have been determined, and in Sanqi, three saponins [22]. In Danshen Injection, seven phenolic acids [23] have also been determined, and in CDT, four diterpenoids [24].…”

Section: Introductionmentioning

confidence: 99%

“…Quantitative analysis of multi-components by single-marker (QAMS) effectively addresses this problem as it requires only a readily available compound to serve as internal reference standard for each class of related compounds. Some research papers have validated the feasibility of QAMS in quality control of TCM [19][20][21][22][23][24][25][26], proving that this method is easy, convenient, and reliable. So far, QAMS has been applied in the quality assessment of Danshen and Sanqi raw herbs and some preparations composed of the two herbs.…”

Section: Introductionmentioning

confidence: 99%

Establishment and validation of quantitative analysis of multi-components by single-marker for quality assessment of compound danshen preparations

Xiong

Liu

Peng

et al. 2014

Acta Chromatographica

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Summary.Compound danshen preparations (CDPs) are used clinically for the treatment of cardiovascular and cerebrovascular diseases. By using the quantitative analysis of multi-components by single-marker (QAMS) method, sixteen compounds (danshensu, protocatechuic acid, protocatechuicaldehyde, caffeic acid, rosmarinic acid, lithospermic acid, notoginsenoside R1, salvianolic acid B, ginsenoside Rg1, ginsenoside Re, salvianolic acid A, salvianolic acid C, ginsenoside Rb1, ginsenoside Rd, cryptotanshinone, and tanshinone IIA were quantified on an ACQUITY ultraperformance liquid chromatography (UPLC) HSS T3 column (2.1 × 100 mm, 1.8 μm) with the mobile phase consisting of 0.1% formic acid aqueous solution (A) and acetonitrile (B) using a gradient elution at the flow rate of 0.30 mL/min in 30 min at 30°C, which was also validated by UPLC-diode array detection (DAD) and UPLC-electrospray ionization multistage/mass spectrometry (ESI-MS/MS) for assuring the feasibility and accuracy. Tested by robustness experiment under slightly changeable conditions, the stability of relative correction factor (RCF) proved to be stable, with RSDs below 5.69%, except for notoginsenoside R1 with relative standard deviation (RSD) 7.83%. This reliable and convenient QAMS method resolved the problem of standard substance insufficiency and improved the quality assessment of preparations consisting of complex compounds with different chemical structures, such as CDPs.

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Ruggedness and robustness of conversion factors in method of simultaneous determination of multi-components with single reference standard

Cited by 110 publications

References 8 publications

A Comprehensive Method for Quality Evaluation of Houttuyniae Herba by a Single Standard to Determine Multi-components, Fingerprint and HPTLC Method

A Comprehensive Method for Quality Evaluation of Houttuyniae Herba by a Single Standard to Determine Multi-components, Fingerprint and HPTLC Method

Fingerprint Analysis and Multi-ingredient Determination Using a Single Reference Standard for Saposhnikoviae Radix

Establishment and validation of quantitative analysis of multi-components by single-marker for quality assessment of compound danshen preparations

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