Salivary biomarkers of oral health – a cross‐sectional study

Rathnayake, Nilminie; Åkerman, Sigvard; Klinge, Björn; Lundegren, Nina; Jansson, Henrik; Tryselius, Ylva; Sorsa, Timo; Gustafsson, Anders

doi:10.1111/jcpe.12038

Cited by 152 publications

(189 citation statements)

References 45 publications

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“…However, there is only one previous study where low salivary flow rate has been indicated as a predictive factor for high salivary MMP-8 counts [Collin et al, 2000]. Rathnayake et al [2013a] found a weak correlation between BOP and MMP-8 levels using the same material as the current study. The correlation was not mirrored in the current study, which may be explained by the use of different statistical models for the analysis of the material.…”

Section: Discussioncontrasting

confidence: 38%

“…The methodological descriptions below regarding participants, clinical and radiographic examination, saliva sampling and biomarker analysis have been described in previous articles [Lundegren et al, 2012;Rathnayake et al, 2013a].…”

Section: Methodsmentioning

confidence: 99%

“…MMP-8 was analyzed by a time-resolved immunofluorometric assay as described by Rathnayake et al [2013a]. Monoclonal MMP-8-antibodies 8708 and 8706 (Medix Biochemica, Kauniainen, Finland) were used as capture and tracer antibodies, respectively.…”

Section: Analysis Of Mmp-8 and Timp-1mentioning

confidence: 99%

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Caries Correlates Strongly with Salivary Levels of Matrix Metalloproteinase-8

et al. 2014

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The caries process in dentin involves the degradation of both mineral and organic matrix. The demineralization has been demonstrated to be caused by bacterial acids. However, the collagen degradation is considered to be initiated by endogenous proteolytic enzymes, mainly collagenolytic matrix metalloproteinases (MMPs). This paper aims to relate salivary MMP-8 (or salivary collagenase-2) and tissue inhibitor of MMP (TIMP-1) levels to manifest caries in a large number of subjects. A random sample of 451 adults (aged 18-87 years) living in the south of Sweden was included in this study. Standard clinical examinations were performed, and stimulated saliva was collected and analyzed for concentrations of MMP-8, TIMP-1 and total protein, using an immunofluorometric assay, an enzyme-linked immunosorbent assay and the Bradford assay, respectively. Salivary numbers of mutans streptococci and lactobacilli were determined using a chair-side kit. Subjects with manifest caries lesions presented with elevated levels of MMP-8 (p < 0.001) as well as total protein, MMP-8/TIMP-1 ratio, bleeding on probing and plaque index (p = 0.05) compared with subjects without manifest caries. Multiple linear regression analysis with caries as the dependent variable revealed MMP-8 as the only significant explanatory variable (p < 0.001). TIMP-1 was not significant in any case. Using MMP-8 as the dependent variable revealed total protein concentration, caries lesions (p ≤ 0.001) and salivary secretion rate (p = 0.05) as explanatory variables. In conclusion, our data reveal that subjects with manifest caries lesions have elevated levels of salivary MMP-8 relative to subjects with no caries lesions. © 2014 S. Karger AG, Basel

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Section: Discussioncontrasting

confidence: 38%

Section: Methodsmentioning

confidence: 99%

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Caries Correlates Strongly with Salivary Levels of Matrix Metalloproteinase-8

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“…found to differentiate between periodontal health and the conditions of gingivitis and periodontitis 13,14 and plays an important role in periodontal tissue destruction. 13 Furthermore, especially serum MMP-8 and TIMP-1 levels may reflect systemic chronic inflammatory and immune responses.…”

Section: Introductionmentioning

confidence: 99%

Elevated matrix metalloproteinase-8 in saliva and serum in polycystic ovary syndrome and association with gingival inflammation

et al. 2015

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This study aimed to investigate the levels of matrix metalloproteinase-8 (MMP-8) and tissue inhibitors of MMP-1 (TIMP-1) in saliva and serum samples of women with polycystic ovary syndrome (PCOS; n = 80) and matched systemically healthy controls (n = 45), with varying degrees of gingival inflammation. Salivary levels of MMP-8 and the MMP-8/TIMP-1 ratio were significantly elevated in women with PCOS, who also exhibited more gingivitis than systemically healthy women. No major changes were observed in salivary TIMP-1 levels with regard to PCOS. Serum levels of MMP-8 and the MMP-8/TIMP-1 ratio were significantly higher in women with PCOS, irrespective of the presence of gingivitis, while there were no differences in TIMP-1 levels. A positive correlation was indicated between probing depth, bleeding on probing, plaque index and salivary or serum MMP-8 levels or MMP-8/TIMP-1 ratio in the case of PCOS, while a negative such correlation was revealed for TIMP-1 in systemically healthy women. Increased levels of MMP-8 in saliva and serum seem to be more pronounced in women with PCOS, and potentiated in the presence of gingival inflammation. Alterations in MMP/TIMP system triggered by local and systemic inflammation may be implicated in the pathogenesis of PCOS, or the deterioration of its clinical presentation.

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“…Salivary biomarkers are used for screening purposes in epidemiological studies [13] and are being used to monitor and detect various diseases, such as breast cancer [14], lung cancer [15], celiac disease [16], and chronic renal failure [11]. With regard to oral health, three important salivary variables should be investigated: pH, flow velocity, and buffering capacity.…”

Section: Discussionmentioning

confidence: 99%

Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças

Buche¹,

Gusso²,

Torres³

et al. 2017

Rev. Odonto Ciênc. (Online)

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OBJECTIVE: Human saliva has numerous components that are important for maintaining oral health. We investigated the association between iron levels in saliva in children and their caries experience. METHODS: We conducted a case-control study that included 186 healthy children, aged 11-14 years. The decayed-filled teeth/decayed-missing-filled teeth (DMFT) score was estimated according to the Brazilian Oral Health Project. The children were divided into two groups: caries-free group (DMFT=0, n=55) and caries experience group (DMFT≥1, n=131). Saliva was collected from each child using the spitting method and analyzed for stimulated saliva flow (SSF), pH, and iron levels. The SSF, pH, and salivary iron levels were analyzed for statistical assumptions of normality and homoscedasticity and compared between groups. All of the statistical tests were performed using R and SPSS software, with a level of significance of 0.05. RESULTS: No statistically significant differences in mean pH were found between groups (t=-0.0488, p=0.51). Children with caries experience had significantly lower salivary iron levels than caries-free children (w=5088, p<0.0001). The SSF values were significantly different between children with caries experience and children without caries (w=4198, p=0.03). CONCLUSION: Salivary iron levels were significantly lower in children with dental caries experience, suggesting that salivary iron plays a role in maintaining oral health.Key words: Saliva; Salivary iron; Caries Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças RESUMO OBJETIVO: Investigar a associação entre os níveis de ferro na saliva das crianças e sua experiência de cárie. MÉTODOS: Realizamos um estudo caso-controle envolvendo 186 crianças saudáveis de 11 a 14 anos. O número de dentes cariados, perdidos e obturados (CPO-D) foi estimado de acordo com o Projeto Saúde Bucal Brasil (SBBrasil) e as crianças foram divididas em dois grupos: grupo sem cárie (CPO-D=0; n=55) e experiência Grupo de cárie (CPO-D≥1; n=131). A saliva foi coletada de cada indivíduo usando o método de cuspir e analisada quanto ao fluxo estimado de saliva (SSF), pH e concentração de ferro. As variáveis SSF, pH e ferro salivar foram analisadas pelos pressupostos estatísticos de normalidade e homocedasticidade e comparados entre os grupos. Todos os testes foram realizados utilizando os programas estatísticos R (R Core Team, 2016) e SPSS (IBM, EUA), assumindo um nível de significância de 0,05. RESULTADOS: Não houve diferenças estatisticamente significativas entre os meios de pH entre os grupos (t=-0,0488; p=0,51). Em relação ao ferro salivar, as crianças com experiência em cárie apresentaram valores significativamente mais baixos de ferro salivar do que aqueles que não apresentavam caries (w=5088; p<0,0001). Além disso, os valores de SSF foram diferentes entre as crianças com experiência de cárie e aqueles sem cárie (w=4198; p=0,03). CONCLUSÃO: os níveis de ferro salivar foram estatisticamente inferiores em crianças com cárie den...

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Salivary biomarkers of oral health – a cross‐sectional study

Abstract: This investigation shows that IL-1β, MMP-8 and the ratio of MMP-8/TIMP-1 could be used as markers of periodontal disease in larger patient populations.

Cited by 152 publications

References 45 publications

Caries Correlates Strongly with Salivary Levels of Matrix Metalloproteinase-8

Caries Correlates Strongly with Salivary Levels of Matrix Metalloproteinase-8

Elevated matrix metalloproteinase-8 in saliva and serum in polycystic ovary syndrome and association with gingival inflammation

Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças

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