Scanning electron microscopy of rabbit tubes at different intervals after anastomosis using fibrin glue

Dargenio, Ruggero; Ranelletti, Franco O.; Tancredi, Giulietta; Ragusa, G.; Mazzocco, Annibale; Garcea, N

doi:10.1016/0028-2243(88)90012-3

Cited by 7 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electron microscopy revealed that the cilia of the tubal epithelium regained full activity within 2-4 weeks following the anastomosis [10]. Fibrin glue caused fewer pelvic adhesions than did surgery, and the glue was subsequently completely absorbed [10,11]. Combination of Swollin's one-stitch technique with the use of fibrin glue sealant in the tubal anastomosis in wom en gave the same pregnancy rate as the microsurgical tech nique [12].…”

Section: Introductionmentioning

confidence: 86%

“…The postoperative intrauterine pregnancy rate de pends on several factors: the fertility of the woman; her age and hormone profile; the nature of the previous steril ization procedure; the length of the repaired tube, location of the anastomosis and whether the tubal repair was per formed in one or both fallopian tubes; the length of time the anastomosis. Electron microscopy revealed that the cilia of the tubal epithelium regained full activity within 2-4 weeks following the anastomosis [10]. Fibrin glue caused fewer pelvic adhesions than did surgery, and the glue was subsequently completely absorbed [10,11].…”

Section: Introductionmentioning

confidence: 94%

“…In a search for alternative, simpler procedures for tubal anastomosis in the reversal of sterilization, tissue adhe sives have been used in women and in experimental stud ies on rabbits [10][11][12], The materials used included fibrin and thrombin glues. The tissue adhesive cyanoacrylate, which is cheaper and readily available in most hospitals, was not used because some acrylates have been shown to be cytotoxic and carcinogenic.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl®)

Haj

Haj²,

Shasha³

et al. 1994

Gynecol Obstet Invest

View full text Add to dashboard Cite

n-Butyl-2-cyanoacrylate (Histoacryl®) is said to be as effective as microsurgery for tubal anastomosis. In a prospective controlled study at the regional teaching hospital research laboratories, 40 female laboratory rats underwent laparotomy, with ligation of the uterine horns, and reanastomosis by microsurgery or cyanoacrylate tissue adhesive. Main outcome measures were patency of uterine horns following anastomosis, histologic examination of anastomotic sites and pregnancy rate following anastomosis by each of the two methods. Patency rates were 100% with both methods of anastomosis, although foreign-body granulomas were more prominent in horns anastomosed by microsurgery. When cyanoacrylate was used on one horn and the contralateral horn ligated, a 90% pregnancy rate was achieved; when microsurgery was used, the pregnancy rate was 80%. In microsurgically anastomosed tubes there was a longer narrow segment, resulting in a lower number of embryos per rat. Cyanoacrylate tissue adhesive is successful in tubal reanastomosis and has certain advantages over classical microsurgical techniques: fewer foreign-body granulomas occur; there is less narrowing of the uterine horn; less skill is required in its use.

show abstract

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl®)

Haj

Haj²,

Shasha³

et al. 1994

Gynecol Obstet Invest

View full text Add to dashboard Cite

show abstract

“…Re ecting the 1943 ndings of Tarlov et al [38,39] regarding autologus plasma clots, more recent studies suggest that AFG obviates possible immunologic responses to non-AFG and consequently acclerates healing [31,46,[81][82][83]. Many methods of autologous brinogen have been described with varied bonding strengths [21,59,74,75,78,84].…”

Section: Fibrinogen Precipitation/ Autologous Fibrin Gluementioning

confidence: 95%

Sutureless and Reduced Suture Anastomosis of Hollow Vessels With Fibrin Glue: A Review

Detweiler

Fenton

1999

Journal of Investigative Surgery

View full text Add to dashboard Cite

Research in reduced suture fibrin glue (FG) and sutureless FG anastomosis has been lagging behind FG utilization in other surgical fields. A review of the literature for vascular, esophageal, tracheal, gastrointestinal, common bile duct, ureteral, vas deferens, and Fallopian tube FG anastomosis indicates that reduced suture FG and sutureless FG procedures may be performed with less training, reduced operating time, leakage, ischemia, inflammation, and necrosis compared to sutured techniques. Reduced suture FG vascular anastomosis augments early anastomotic strength. Suture number for esophageal, tracheal, and tracheobronchial anastomoses can be reduced with FG. Bursting strength in pig small intestine and rat colon was lower at 4 days postoperatively, but returned to sutured strength at 7 days. Mortality was unaffected, and 18-month follow-up in sutureless FG intestinal anastomosis in pigs showed no stenosis. Preliminary ureteral studies have demonstrated successful sutureless FG and reduced suture FG laparoscopic techniques in pigs. Reduced suture FG and sutureless FG vas deferens anastomosis may reduce sperm granuloma rates, with increased patency and pregnancy rates. Patency and pregnancy rates have been similar for tubal FG, reduced suture FG, autologous fibrin glue (AFG), and sutured anastomosis. Any risk of viral transmission or immune response is eliminated by AFG. While there are few studies in many areas of FG hollow vessel anastomosis, the current literature illustrates many of the advantages of FG over other anastomotic techniques and should provide impetus for continued research in this promising field of surgery.

show abstract

“…Our granu lomas were epithelioid with abundant eosino philic leukocytes. Dargenio et al [10] used scanning electron microscopy to examine the reepithelialization and ciliogenesis after tubal anastomosis in rabbits. They used human fibrin adhesive from Immuno.…”

Section: Discussionmentioning

confidence: 99%

Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Weis-Fogh

Pedersen²,

Schroeder³

et al. 1993

Eur Surg Res

View full text Add to dashboard Cite

The morphology of the healing process of microsurgical re-anastomosis of the rabbit oviduct with the use of fibrin adhesive, autologous and heterologous, and conventional sutures is described. Both oviducts in 48 rabbits were cut and reanastomoses were performed. The rabbits were killed at different intervals after the operations, ranging from 2 h to 28 days, and the anastomoses were evaluated by histomorphological examination.The autologous fibrin adhesive was absorbed after a week and an uncomplicated healing was observed. Heterologous fibrin adhesive caused a granulomatous inflammation interpreted as an immune reaction of the host to the foreign protein, and conventional suturing resulted in severe tissue damage with an intensive inflammatory reaction.

show abstract

Scanning electron microscopy of rabbit tubes at different intervals after anastomosis using fibrin glue

Cited by 7 publications

References 7 publications

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl®)

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl®)

Sutureless and Reduced Suture Anastomosis of Hollow Vessels With Fibrin Glue: A Review

Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Contact Info

Product

Resources

About

Scanning electron microscopy of rabbit tubes at different intervals after anastomosis using fibrin glue

Cited by 7 publications

References 7 publications

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl&reg;)

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl&reg;)

Sutureless and Reduced Suture Anastomosis of Hollow Vessels With Fibrin Glue: A Review

Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Contact Info

Product

Resources

About

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl®)

Tubal Anastomosis in the Rat Using the Tissue Adhesive Cyanoacrylate (Histoacryl®)