Scar Prevention and Enhanced Wound Healing Induced by Polydeoxyribonucleotide in a Rat Incisional Wound-Healing Model

Jeong, Woonhyeok; Yang, Chae Eun; Roh, Tai Suk; Kim, Jun Hyung; Lee, Ju Hee; Lee, Won Jai

doi:10.3390/ijms18081698

Cited by 65 publications

(52 citation statements)

References 33 publications

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“…Effect of PDRN size on wound closure in mice skin. Multiple previous studies have demonstrated that PDRNs promote wound healing in various animal models (6,10,(18)(19)(20). To evaluate the effect of PDRNs on wound healing, 8-week old hairless (SKH1) mice were given different sizes of PDRNs daily for a week.…”

Section: Resultsmentioning

confidence: 99%

An effective range of polydeoxyribonucleotides is critical for wound healing quality

et al. 2018

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Wound healing is a physiological restorative response to tissue and cell injury. This process occurs in collaboration with a complex cascade of cellular events, including biochemical alterations to the extracellular matrix. Polydeoxyribonucleotide (PDRN) is a fragmented DNA mixture from Oncorhynchus mykiss or Oncorhynchus keta sperm known to promote tissue regeneration under different pathophysiological conditions. However, the most effective molecular size of PDRNs for promoting the wound healing process and quality has not been established. In the present study, the regeneration quality with low (<50 kDa), middle [classic PDRN; 50-1,500 kDa] and high (>1,500 kDa) molecular weight PDRNs in a skin wound healing mouse model was examined using hematoxylin and eosin, as well as Masson's trichrome stain. A 4 mm biopsy punch was used to produce wounds in the skin of the mice. PDRN-mediated cellular behavior and signaling were evaluated by in vitro scratch assay and western blot analysis, respectively. It was observed that the apparent surface wound healing processes were not significantly different between PDRN molecular sizes. Immunohistochemical analysis revealed that classic PDRN-injected mice exhibited less lipid accumulation with increased collagen composition. These results suggested that 50-1,500 kDa PDRN offers an effective DNA mixture to improve wound healing quality. Furthermore, classic PDRN increased cell migration via c-Jun N-terminal kinase signaling in human fibroblasts. The present study suggests an optimal PDRN molecular weight to promote wound healing, and novel approaches for therapeutic strategies to improve tissue regeneration quality.

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Section: Resultsmentioning

confidence: 99%

An effective range of polydeoxyribonucleotides is critical for wound healing quality

et al. 2018

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“…PRP contains high amounts of platelets, and owing to the fact that platelets release some of the growth factors involved in modulating the regeneration process, it is not surprising that the MSC-devoid FMs still have a regenerative effect on the VML site. The inflammation phase in the FM-R transplantation groups (with/without MSCs) passed faster than the Sham group [55]. Growth factors released from FM-Rs may also have an influence in decreasing scar tissue formation.…”

Section: Hande Histochemical Findings: Fibrosis or Regenerationmentioning

confidence: 96%

Encapsulation of bone marrow-MSCs in PRP-derived fibrin microbeads and preliminary evaluation in a volumetric muscle loss injury rat model: modular muscle tissue engineering

Lalegül‐Ülker

Şeker

Elçin

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Repair of volumetric muscle loss (VML) injuries is a complicated endeavour which necessitates the collaborative use of different regenerative approaches and technologies. Herein is proposed the development of fibrin-based microbeads (FMs) alone or as a bone marrow mesenchymal stem cell (MSC) encapsulation matrix for modular muscle engineering. FMs were generated through the ionotropic gelation of alginate and fibrinogen obtained from the platelet-rich plasma of whole blood, and then removing the alginate by citrate treatment. FMs were first characterized by FT-IR, SEM and water uptake tests. Then, the stability of FMs and the mitochondrial dehydrogenase activity of the MSCs encapsulated in FMs were evaluated under in vitro culture conditions. Eventually, the regenerative capacity of the cell-devoid and MSCs-encapsulated FMs was evaluated in a rat VML injury model involving 8 Â 4Â4 mm 3-size bilateral defects in the biceps femoris muscles. The histochemical, immunohistochemical and semi-quantitative histomorphological scoring results retrieved at 30, 60 and 180 days demonstrated that the cell-devoid FMs supported muscle regeneration to a great extent. Moreover, MSCsencapsulated FMs were more effective in shortening the regeneration period of the injured tissue of the rat VML, resulting in good myofibre orientation, while the Sham group resulted in incomplete repair with fibrotic scar tissue formations.

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“…Some of these same mediators have also been studied in adult wound healing models. For example, HMGB-1 has been linked to scar formation [ 88 ] and blocking PGE 2 production with drugs that inhibit cyclooxygenase-2 activity has been shown to reduce scar formation in adult incisional wound models [ 89 , 90 ] . Other pro-inflammatory cytokines that have been linked to cutaneous scar formation and/or collagen production in adult scar/fibrosis models include IL-17 [ 91 ] and monocyte chemoattractant protein-1 [ 92 , 93 ] .…”

Section: Functional Data Linking Inflammation and Scarringmentioning

confidence: 99%

Inflammation as an orchestrator of cutaneous scar formation: a review of the literature

Wilgus

2020

Plast Aesthet Res

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Inflammation is a key phase in the cutaneous wound repair process. The activation of inflammatory cells is critical for preventing infection in contaminated wounds and results in the release of an array of mediators, some of which stimulate the activity of keratinocytes, endothelial cells, and fibroblasts to aid in the repair process. However, there is an abundance of data suggesting that the strength of the inflammatory response early in the healing process correlates directly with the amount of scar tissue that will eventually form. This review will summarize the literature related to inflammation and cutaneous scar formation, highlight recent discoveries, and discuss potential treatment modalities that target inflammation to minimize scarring.

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Scar Prevention and Enhanced Wound Healing Induced by Polydeoxyribonucleotide in a Rat Incisional Wound-Healing Model

Cited by 65 publications

References 33 publications

An effective range of polydeoxyribonucleotides is critical for wound healing quality

An effective range of polydeoxyribonucleotides is critical for wound healing quality

Encapsulation of bone marrow-MSCs in PRP-derived fibrin microbeads and preliminary evaluation in a volumetric muscle loss injury rat model: modular muscle tissue engineering

Inflammation as an orchestrator of cutaneous scar formation: a review of the literature

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