Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants

Chakraborty, Somnath; Ghosh, Upasana; Balasubramanian, T.; Das, Punyabrata

doi:10.12980/apjtb.4.2014c1037

Cited by 25 publications

(22 citation statements)

References 43 publications

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“…Further, it can be said that with 1000 mg/kg of body weight/day of MP07X through oral increased the survival rate of vannamei shrimp up to of 85%. The previous report stated that MP07X was not toxic to shrimp against WSSV at an effective doses (Chakraborty & Ghosh, 2013b).…”

Section: The Mortality Of Tiger Shrimpmentioning

confidence: 94%

The effectiveness of methanol extract and fractinations fromleaves of mangroveSonneratia albaand Bruguiera gymnorrhizafor the prevention of white spot syndrome virus (WSSV) infection in black tiger shrimp Penaeus monodon

Muliani

Susianingsih

2018

Jurnal Akuakultur Indonesia

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ABSTRACT The experiment aimed to determine the effectiveness of mangrove leaves (Sonneratia alba and Bruguiera gymnorrhiza) extracts for prevention of WSSV infection in black tiger shrimp, Penaeus monodon. Mangrove leaves (S.alba and B. gymnorrhiza) were taken from Maros and Pangkep Regency, respectively. Mangrove leaves dried approximately for two<ins cite="mailto:reviewer" datetime="2018-03-05T09:28"> </ins>weeks, made into flour, extracted using 80% methanol, and then evaporated. The yield partitioned using two types of solvents, theywere butanol and dietyleter. Anti WSSV activity test was done by injection method with the ratio of mangrove extract and WSSV was 2:1 as much as 100 µL/ind. The treatment were; A). Water fraction of S. alba + WSSV suspention; B). Butanol fraction of S. alba + WSSV suspention; C). Dietyleter fraction of S. alba+ WSSV suspention; D). Methanol extract of S. alba + WSSV suspention; E). Water fraction of B. gymnorrhiza + WSSV suspention; F). Butanol fraction of B. gymnorrhiza + WSSV suspention; G). Dietyleter fraction of B. gymnorrhiza + WSSV suspention; H). Methanol extract of B. gymnorrhiza + WSSV suspention; I). Positive control (WSSV suspention without mangrove extract). Each treatment was repeated three times with 10 days of rearing period. The results showed that the highest activity of anti-WSSV from S. alba was in dietyleter fraction and the methanol extract, while the highest activity of anti-WSSV from B. gymnorrhiza was in butanol fraction. The dietyleter fraction and the methanol extract of S. alba and the butanol fraction of B. gymnorrhiza were capable to stimulate immune response of shrimp and were effective in deactivating WSSV furthermore it increased the black tiger shrimp survival rateto 100%. Keywords: antiviral, white spot syndrome virus (WSSV), mangrove herbs, S. alba, B. gymnorrhiza, Penaeus monodon ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak dari daun mangroveSonneratia. alba dan Bruguiera gymnorrhizauntuk pencegahan infeksi WSSV pada udang windu, Penaeus monodon. Daun mangroveS.alba dan B. gymnorrhiza masing-masing diambil dari Kabupaten Maros dan Kabupaten Pangkep. Daun mangrove dikering anginkan selama dua minggu, dibuat tepung, diekstraksi dengan metanol 80%, dan dievaporasi. Rendemen yang diperoleh dipartisi menggunakan dua jenis pelarut yaitu butanol dan dietileter. Uji aktivitas anti WSSV dilakukan metode penyuntikan dengan perbandingan ekstrak mangrove dengan WSSV 2:1sebanyak 100 µL/ekor. Perlakuan yang diujikan adalah; A). Fraksi air S. alba + suspensi WSSV; B). Fraksi butanol S. alba + suspensi WSSV; C). Fraksi dietileter S. alba+ suspensi WSSV; D). Ekstrak metanol S. alba+ suspensi WSSV; E). Fraksi air B. gymnorrhiza + suspensi WSSV; F). Fraksi butanol B. gymnorrhiza + suspensi WSSV; G). Fraksi dietileter B. gymnorrhiza + suspensi WSSV; H). Ekstrak metanol B. gymnorrhiza + suspensi WSSV; I). Kontrol positif (suspensi WSSV tanpa ekstrak mangrove). Masing-masing perlakuan diulang tiga kali dengan lama pemeliharaan 10 hari. Hasil penelitian menunjukkan bahwa aktivitas anti WSSV S. alba tertinggi pada fraksi dietileter dan ekstrak metanol, sedangkan aktivitas anti WSSV B. gymnorrhiza tertinggi pada fraksi butanol. Fraksi dietileter dan ekstrak metanol S. albaserta fraksi butanol B. gymnorrhiza mampu menstimulasi respons immun udang dan efektif dalam menonaktifkan WSSV sehingga meningkatkan sintasan udang windu hingga 100%. Kata Kunci: anti viral, white spot syndrome virus (WSSV), herbal mangrove, S alba, B. gymnorrhiza, udang windu

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Section: The Mortality Of Tiger Shrimpmentioning

confidence: 94%

The effectiveness of methanol extract and fractinations fromleaves of mangroveSonneratia albaand Bruguiera gymnorrhizafor the prevention of white spot syndrome virus (WSSV) infection in black tiger shrimp Penaeus monodon

Muliani

Susianingsih

2018

Jurnal Akuakultur Indonesia

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“…Pemanfaatan mangrove sebagai antibakteri pada udang putih dan ikan hias juga telah dilaporkan (Arivuselvan et al, 2011;Dhayanithi et al, 2013), dan pada ikan mas (Ahilan et al, 2010). Selain sebagai anti bakteri, tanaman herbal mangrove dan asosiasinya juga mulai dikaji sebagai anti WSSV pada udang (Sahu et al, 2012;Chakraborty & Ghosh, 2013a, 2013b.…”

Section: Pendahuluanunclassified

HERBAL MANGROVE SEBAGAI SUMBER ANTI BAKTERI Vibrio harveyi PENYEBAB PENYAKIT PADA UDANG WINDU Penaeus monodon

Muliani

Nurhidayah

Kurniawan

2015

Jurnal Riset Akuakultur

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ABSTRAKPenelitian ini bertujuan untuk mendapatkan herbal tanaman mangrove sebagai sumber antibakteri V. harveyi penyebab penyakit pada udang windu Penaeus monodon. Penelitian dilakukan di Balai Penelitian dan Pengembangan Budidaya Air Payau (BPPBAP), Maros. Mangrove diambil dari lima lokasi yaitu: Kabupaten Maros, Pangkep, Luwu Timur, Takalar, dan Bone. Mangrove yang dikumpulkan diidentifikasi sebelum dikering-anginkan selama dua minggu. Setelah kering dibuat tepung dan diekstrak menggunakan pelarut organik (metanol). Ekstrak metanol dari herbal mangrove diuji bioassay secara kualitatif dan kuantitatif untuk mengetahui aktivitas anti V. harveyi menggunakan metode mikrowell plate. Setelah itu, dilakukan uji tantang secara in vitro dengan V. harveyi pada konsentrasi yang berbeda dan selanjutnya dilakukan penentuan konsentrasi ambang bawah dan ambang atas yang mematikan terhadap larva udang windu. Hasil penelitian menunjukkan bahwa dari 88 sampel herbal mangrove yang dikumpulkan 58 (65,91%) sampel positif mengandung antibakteri V. harveyi, yang terdiri atas: 11 (18,96%) dari Maros, 16 (27,59%) dari Pangkep, 11 (18,96%) dari Luwu Timur, 4 (6,90%) dari Takalar, dan 16 (27,59%) dari Bone. Minimum Inhibition Concentration (MIC) terbaik ditunjukkan oleh lima jenis tanaman mangrove yaitu; Sonneratia lanceolata dengan nilai MIC 0,1 mg/L dan Sonneratia alba, Sonneratia caseolaris, Bruguiera gymnorrhiza, serta Rhizophora mucronata dengan nilai MIC masing-masing 1 mg/L. Hasil uji tantang secara in vitro dengan V. harveyi menunjukkan bahwa S. alba dan S. caseolaris terbaik menghambat pertumbuhan V. harveyi. Konsentrasi 10.000 mg/L dari ekstrak metanol S. lanceolata, S. alba, S. caseolaris, serta B. gymnorrhiza sudah toksik bagi benur udang windu, dengan mortalitas mencapai 100% setelah 96 jam. KATA KUNCI: mangrove, herbal, antibakteri, V. harveyi, vibriosis, Penaeus monodon ABSRTACT: Mangrove herbs as a source of antibacterial V. harveyi causes disease in black tiger shrimp Penaeus monodon. By: Muliani, Nurhidayah, and Koko Kurniawan This experiment was aimed to findout of mangrove herbs as a source of antibacterial V. harveyi causes disease in black tiger shrimp Penaeus monodon. The experiment was conducted in several stages, namely: a) collection and identification of mangrove plants, b) preparation and drying plant mangrove; c) preparation of starch; d) extraction and evaporate; e) qualitative bioassay test; f) quantitative bioassay test; g) in vitro test challenged with V. harveyi; h) toxicity tests against larvae of tiger shrimp. The result showed that as many as 88 samples of mangrove herbs collected from Maros, Pangkep, Luwu Timur, Takalar, and Bone, 58 (65.91%) of them positive for antibacterial V. harveyi, which consisted of 11 (18.96%) samples were from Maros, 16 (27.59%) samples were from Pangkep, 11 (18.96%) samples were from Luwu Timur, 4 (6.90%) samples were from Takalar, and 12 (2.59%) samples were from Bone. Minimum Inhibition Concentration (MIC) best indicated by four mangrove species namely; Sonneratia lanceo...

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“…However, as invertebrates, shrimp lack an adaptive immune system, rendering vaccination ineffective. As a result, many antiviral approaches that have been studied rely on enhancing host innate immunity [ 2 ]; examples include oral administration of probiotics [ 3 ], peptidoglycans [ 4 ] of β-glucan [ 5 ] and plant or seaweed extracts [ 6 ]. Other studies revealed that shrimp was capable of deploying an antiviral immune response via the RNA interference (RNAi) pathway [ 7 , 8 ], as evidenced by the finding that injection of double-stranded RNA (dsRNA) constructs with sequences that matched viral mRNA was effective in preventing viral diseases or reducing their severity [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Co-expression of double-stranded RNA and viral capsid protein in the novel engineered Escherichia coli DualX-B15(DE3) strain

et al. 2021

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Background Viruses cause significant economic losses to shrimp aquaculture worldwide. In severe cases, they can lead to 100% mortality within a matter of days, hence the aquaculture industry requires antiviral strategies to minimize economic impacts. Currently, a double-stranded RNA (dsRNA)-based platform has been proven effective at a laboratory scale. The bottleneck for its industrialization is the lack of low-cost, efficient and practical delivery approaches. In an effort to bridge the gap between laboratory and farm applications, virus-like particles (VLP) have been used as nanocarriers of dsRNA. However, the implementation of this approach still suffers from high costs and a lengthy procedure, co-expression of subunits of VLP or capsid proteins (CPs) and dsRNA can be the solution for the problem. CP and dsRNA are traditionally expressed in two different E. coli hosts: protease-deficient and RNase III-deficient strains. To condense the manufacturing of dsRNA-containing VLP, this study constructed a novel E. coli strain that is able to co-express viral capsid proteins and dsRNA in the same E. coli cell. Results A novel bacterial strain DualX-B15(DE3) was engineered to be both protease- and RNase III-deficiency via P1 phage transduction. The results revealed that it could simultaneously express recombinant proteins and dsRNA. Conclusion Co-expression of viral capsid proteins and dsRNA in the same cell has been shown to be feasible. Not only could this platform serve as a basis for future cost-effective and streamlined production of shrimp antiviral therapeutics, it may be applicable for other applications that requires co-expression of recombinant proteins and dsRNA.

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Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants

Abstract: The drug MP07X derived from Rhizophora mucronata is a potent anti-WSSV drug.

Cited by 25 publications

References 43 publications

The effectiveness of methanol extract and fractinations fromleaves of mangroveSonneratia albaand Bruguiera gymnorrhizafor the prevention of white spot syndrome virus (WSSV) infection in black tiger shrimp Penaeus monodon

The effectiveness of methanol extract and fractinations fromleaves of mangroveSonneratia albaand Bruguiera gymnorrhizafor the prevention of white spot syndrome virus (WSSV) infection in black tiger shrimp Penaeus monodon

HERBAL MANGROVE SEBAGAI SUMBER ANTI BAKTERI Vibrio harveyi PENYEBAB PENYAKIT PADA UDANG WINDU Penaeus monodon

Co-expression of double-stranded RNA and viral capsid protein in the novel engineered Escherichia coli DualX-B15(DE3) strain

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