Screening of NO Inhibitor Release Activity from Soft Coral  Extracts Origin Palu Bay, Central Sulawesi, Indonesia

Tanod, Wendy Alexander; Yanuhar, Uun; Maftuch,; Putra, Masteria Yunovilsa; Risjani, Yenny

doi:10.2174/1871523018666190222115034

Cited by 17 publications

(19 citation statements)

References 95 publications

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“…In previous studies, it was concluded that the three soft coral crude extracts used in this study had nitric oxide (NO) inhibitory release activity (Tanod, Yanuhar, Maftuch, Putra, & Risjani, 2019). Besides that, the three soft coral crude extracts showed DPPH scavenging properties at concentrations 10, 30, 50, 70, 90 g/mL (Tanod, Yanuhar, Maftuch, Wahyudi, & Risjani, 2019).…”

Section: Introductionmentioning

confidence: 63%

Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Tanod

Dewanto

Ndobe

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

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This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.

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Section: Introductionmentioning

confidence: 63%

Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Tanod

Dewanto

Ndobe

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

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“…The previous study showed that DCM extracts of Sarcophyton spp. inhibited NO production [14]. This inhibition of NO production was presumably achieved by regulating the level of iNOS and its upstream molecules in LPS-induced macrophage cells [28].…”

Section: Discussionmentioning

confidence: 98%

“…The previous studies have also reported the ability of the dichloromethane (DCM) extract of Sarcophyton spp. (collected from Palu Bay, Central Sulawesi, Indonesia) to inhibit NO release [14]. In addition, soft coral Sarcophyton spp.…”

Section: Introductionmentioning

confidence: 99%

Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice

2019

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Background and Aim: The soft coral genus Sarcophyton is a source of cembraneterpen. Sarcophyton is reported to have anti-inflammatory properties, with the ability to reduce the expression of inducible nitric oxide synthase (iNOS) and inhibit nuclear factor-kappa B (NF-κB) activation. This study aimed to investigate the efficacy of dichloromethane (DCM) extracts of soft coral Sarcophyton spp. to inhibit the expression of NF-κB and iNOS induced by lipopolysaccharide (LPS). Materials and Methods: Crude extracts of Sarcophyton spp. were macerated with DCM (1:3 v/v) for 24 h. Thirty-six Balb/c mice were divided into six treatment groups, namely, normal control (without LPS induction), negative control (LPS induction 4 mg/mL), comparative control (LPS+Dexamethasone 6 mg/kg), and 3 concentration groups extract (LPS+50, 125, and 250 mg/kg). The expression of NF-κB and iNOS was measured in each treatment group. Results: Flow cytometry analysis showed that the relative number of NF-κB+ cells increased (18.38±1.24%) in LPS-induced mice compared with normal mice (13.24±1.15%). The Sarcophyton spp. DCM extracts decreased the relative number of NF-κB+ cells (125 mg/kg: 13.96±0.84%). Immunohistochemical analysis with ImmunoMembrane showed that LPS induction in mice increased iNOS expression when compared to normal mice. The Sarcophyton spp. DCM extracts reduced iNOS expression (especially at 125 mg/kg). Conclusion: DCM extracts of Sarcophyton spp. inhibited the activation of NF-κB, resulting in suppressed iNOS expression, which directly inhibits NO production.

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“…Metode broth-dilution berdasarkan pedoman EUCAST (2003) dan Wiegand et al (2008) (Tanod et al, 2019c). Rachmaniar (1995) menyatakan bahwa saponin merupakan metabolit dengan karakteristik rasa pahit dan bersifat toksik bagi amfibi dan ikan, serta memiliki fungsi farmakologis.…”

Section: Pengujian Antibakteri Dengan Metode Brothdilutionunclassified

AKTIVITAS ANTIBAKTERI EKSTRAK KARANG LUNAK Sinularia sp. DENGAN METODE BROTH-DILUTION

Salanggon

Aswani

Hasanuddin

et al. 2020

Jurnal Kelautan Nasional

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Karang lunak merupakan bagian dari ekosistem terumbu karang, yang mengandung senyawa bioaktif dan dapat berperan sebagai alat pertahanan diri serta memiliki kemampuan sebagai senyawa antibakteri. Penelitian ini bertujuan untuk menginvestigasi potensi ekstrak karang lunak Sinularia sp. sebagai antibakteri dengan metode broth-dilution. Karang Lunak Sinularia sp. dikoleksi dari perairan Desa Kabonga Besar, Sulawesi Tengah, dan dimaserasi dengan diklorometana : methanol. Ekstrak Sinularia sp. dilakukan pengujian skrining metabolit kimia dan pengujian antibakteri dengan metode broth-diluttion. Hasil skrining ekstrak Sinularia sp. terdeteksi senyawa metabolit flavonoid, saponin, alkaloid dan steroid. Ekstrak Sinularia sp. memberikan pengaruh terhadap total koloni Escherichia coli dan Pseudomonas aeruginosa. Ekstrak Sinularia sp. dapat menghambat pertumbuhan E. coli dengan konsentrasi minimum 1.76 ± 0.10 mg/mL, sedangkan pada P. aeruginosa konsentrasi minimum yang dibutuhkan 1.85 ± 0.14 mg/mL. Konsentrasi bunuh minimum ekstrak Sinularia sp. yang dibutuhkan dalam membunuh E. coli, yaitu 7.06 ± 0.42 mg/mL, sedangkan pada P. aeruginosa, yaitu 7.38 ± 0.54 mg/mL. Penelitian ini disimpulkan bahwa senyawa bioaktif dari metabolit ekstrak karang lunak Sinularia sp. berpotensi sebagai senyawa antibakterial terhadap E. coli dan P. aeruginosa. Oleh karena itu, perlu diinvestigasi lebih lanjut dari identifikasi profil bioaktif karang lunak Sinularia sp.

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Screening of NO Inhibitor Release Activity from Soft Coral Extracts Origin Palu Bay, Central Sulawesi, Indonesia

Cited by 17 publications

References 95 publications

Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice

AKTIVITAS ANTIBAKTERI EKSTRAK KARANG LUNAK Sinularia sp. DENGAN METODE BROTH-DILUTION

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