Seasonal changes in proteomic profiles of Japanese kelp: Saccharina japonica (Laminariales, Phaeophyceae)

Abstract

The seasonal variation in protein expression in the sporophyte of Saccharina japonica (Areschoug) Lane, Mays, Druehl and Saunders was investigated. High-quality proteins that are available for protein profiling were extracted by the ethanol/phenol extraction method, and 564 protein spots in total were detected. Proteins were identified through database search by combining Mascot and MS BLAST for 100 spots, and significant difference of expression level between the samples collected in winter and in summer was observed in the case of 95 spots. Within 67 spots upregulated in the samples collected in summer, vanadium-dependent bromoperoxidase (vBPO) were identified for 21spots. It is thought that the elevation of expression level of vBPO in summer depend on the activation of the functions: (1) elimination of active oxygen species and protection of the algal body from oxygen injury, (2) prevention of the growth inhibition due to the adherence of attached organisms, in the season.

Temperature stress-induced changes in the proteomic profiles of Ecklonia cava (Laminariales, Phaeophyceae)

“…Recently, clear protein spots have been obtained by two-dimensional gel electrophoresis (2-DE) of the proteins extracted (ethanol/phenol) from the sporophyte of Ecklonia kurome, a warm-sea kelp (Nagai et al 2008). More recently, seasonal variations in the proteomic profiles of the sporophyte of Saccharina japonica, a cold-sea kelp, have been detected with 2-DE by using the same extractionseparation method mentioned above (Yotsukura et al 2010). The results in the latter study indicate that the expression of 67 spots increased in summer and that of 28 spots increased in winter.…”

Influences of hydrozoan colonization on proteomic profiles of the brown alga Saccharina japonica

“…Among them, 69 and 32 spots were up-and down-regulated, respectively. When blades of S. japonica were collected in different seasons, summer blades showed 67 up-and 28 down-regulated spots compared to winter samples (Yotsukura et al 2010). Three and six distinctive protein spots were upand down-regulated, respectively, when S. japonica was incubated at pH 9.5 and at pH 8.5 (Kim et al 2011).…”

“…Protein profiling using proteomics can be used to identify marker proteins that are up-or down-regulated in response to environmental stresses or parasite colonization. Recently, proteomic profiles from S. japonica were identified with seasonal changes (Yotsukura et al 2010) and different incubation conditions (Kim et al 2011). The proteomic profiles (Getachew et al 2014) and biochemical composition (Getachew et al 2015b) of S. japonica upon bryozoan colonization were also analyzed.…”

Proteomics in Seaweeds: Ecological Interpretations

“…The work described by Yotsukura et al (2010) presents a similar protocol to the one described by Contreras et al (2008). Here, proteins are extracted from the brown alga Saccharina japonica (Chromista, Ochrophyta), important kelp described principally on the coastal areas of northern Japan.…”