Second Messengers Mediate Increases in Cytosolic Calcium in Tobacco Protoplasts

Volotovski, I. D.; Sokolovsky, Sergei G.; Молчан, О. В.; Knight, Marc R.

doi:10.1104/pp.117.3.1023

Cited by 104 publications

(63 citation statements)

References 44 publications

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“…Swelling of tobacco (Nicotiana tabacum) protoplasts is induced by cGMP (Volotovski et al, 1998). Similarly, in this study, 50 mM of the membrane-permeable active cGMP variant 8-Br cGMP induced swelling of protoplasts from wild-type Arabidopsis seedlings.…”

Section: Cngc17 Is Required For Psk-induced Protoplast Expansionsupporting

confidence: 74%

“…In this study, we provide evidence that PSK promotes cell expansion via cGMP as a second messenger that activates the cation channel CNGC17 as its target. cGMP was previously shown to promote swelling of tobacco protoplasts (Volotovski et al, 1998) and stoma opening in Tradescantia albiflora, while inhibition of GC caused stoma closure, supporting a role for GC activity in guard cell expansion (Pharmawati et al, 1998(Pharmawati et al, , 2001). We further show that the PSK receptor PSKR1 interacts with the PM-localized ATPase isoforms AHA1 and AHA2, which in turn physically interact with CNGC17, thus providing indirect linkage between PSKR1 and the cGMP target CNGC17.…”

Section: Discussionmentioning

confidence: 81%

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Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H⁺-ATPase, and BAK1

et al. 2015

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Phytosulfokine (PSK) is perceived by the leucine-rich repeat receptor kinase PSKR1 and promotes growth in Arabidopsis thaliana. PSKR1 is coexpressed with the CYCLIC NUCLEOTIDE-GATED CHANNEL gene CNGC17. PSK promotes protoplast expansion in the wild type but not in cngc17. Protoplast expansion is likewise promoted by cGMP in a CNGC17-dependent manner. Furthermore, PSKR1-deficient protoplasts do not expand in response to PSK but are still responsive to cGMP, suggesting that cGMP acts downstream of PSKR1. Mutating the guanylate cyclase center of PSKR1 impairs seedling growth, supporting a role for PSKR1 signaling via cGMP in planta. While PSKR1 does not interact directly with CNGC17, it interacts with the plasma membrane-localized H + -ATPases AHA1 and AHA2 and with the BRI-associated receptor kinase 1 (BAK1). CNGC17 likewise interacts with AHA1, AHA2, and BAK1, suggesting that PSKR1, BAK1, CNGC17, and AHA assemble in a functional complex. Roots of deetiolated bak1-3 and bak1-4 seedlings were unresponsive to PSK, and bak1-3 and bak1-4 protoplasts expanded less in response to PSK but were fully responsive to cGMP, indicating that BAK1 acts in the PSK signal pathway upstream of cGMP. We hypothesize that CNGC17 and AHAs form a functional cation-translocating unit that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes.

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Section: Cngc17 Is Required For Psk-induced Protoplast Expansionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 81%

Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H⁺-ATPase, and BAK1

et al. 2015

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“…17 For example, plant AC is closely associated with signaling cascade for pollen tube growth, 14 and cAMP have been shown to regulate Ca 2+ signaling in N. plumbaginofolia protoplasts. 20 In addition, it has been reported that self-incompatibility is associated with the level of cAMP in pistils after pollination, and the level of cAMP depends on AC activity. 21 AC and cAMP are also related to plant immune responses, and cAMP is transiently elevated in elicited bean cells.…”

Section: Discussionmentioning

confidence: 99%

Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfire disease inNicotiana benthamiana

Ito

Takahashi

Sawasaki

et al. 2014

Plant Signaling & Behavior

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“…cAMP signaling has been a controversial issue in plant cells despite occasional reports of gene homologs for elements of this pathway (24) and responses induced by modulators of AC activity (25,26). Previous methodologies used to measure cAMP levels were often found to provide values close to or below the detection limit (12).…”

Section: Discussionmentioning

confidence: 99%

cAMP acts as a second messenger in pollen tube growth and reorientation

Moutinho

Hussey

Trewavas

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

221

209

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Pollen tube growth and reorientation is a prerequisite for fertilization and seed formation. Here we report imaging of cAMP distribution in living pollen tubes microinjected with the protein kinase A-derived fluorosensor. Growing tubes revealed a uniform distribution of cAMP with a resting concentration of Ϸ100 -150 nM. Modulators of adenylyl cyclase (AC), forskolin, and dideoxyadenosine could alter these values. Transient elevations in the apical region could be correlated with changes in the tube-growth axis, suggesting a role for cAMP in polarized growth. Changes in cAMP arise through the activity of a putative AC identified in pollen. This signaling protein shows homology to functional motifs in fungal AC. Expression of the cDNA in Escherichia coli resulted in cAMP increase and complemented a catabolic defect in the fermentation of carbohydrates caused by the absence of cAMP in a cyaA mutant. Antisense assays performed with oligodeoxynucleotide probes directed against conserved motifs perturbed tip growth, suggesting that modulation of cAMP concentration is vital for tip growth.

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Second Messengers Mediate Increases in Cytosolic Calcium in Tobacco Protoplasts

Cited by 104 publications

References 44 publications

Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H⁺-ATPase, and BAK1

Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H⁺-ATPase, and BAK1

Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfire disease inNicotiana benthamiana

cAMP acts as a second messenger in pollen tube growth and reorientation

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Second Messengers Mediate Increases in Cytosolic Calcium in Tobacco Protoplasts

Cited by 104 publications

References 44 publications

Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H+-ATPase, and BAK1

Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H+-ATPase, and BAK1

Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfire disease inNicotiana benthamiana

cAMP acts as a second messenger in pollen tube growth and reorientation

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Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H⁺-ATPase, and BAK1

Phytosulfokine Regulates Growth in Arabidopsis through a Response Module at the Plasma Membrane That Includes CYCLIC NUCLEOTIDE-GATED CHANNEL17, H⁺-ATPase, and BAK1