This study was conducted to determine the Ribavirin in Environmental and Biological Samples via Optical Chemical Sensor. In this paper, a new magnetic metal-organic framework Fe O @Fe-ASB was synthesized 3 4 with nano-Fe O as metal center and a new Schiff base, which was synthesized from quinoline-2-3 4 formaldehyde and β-mercaptoethylamine, as ligand, This Fe O @Fe-ASB was characterized by FITR, SEM 3 4 and XRD. Its enzyme-like activity was characterized by the chromogenic substrate (H O and TMB) and 2 2 verified based on the Michaelis-Menten equation. The results showed that the synthesis of Fe O @Fe-ASB was successful and it exhibited excellent 3 4 enzyme-like activity. The Fe O @Fe-ASB was successfully used for the detection of ribavirin in water 3 4 samples as catalytic material. It showed good stability, accuracy and precision. This testing method features a graph, which plots concentration (c) on the x-axis and absorption A on the y-axis to create a linear ribavirin-4-1-6-1 equation. It shows a good linear relation ranging from 1×10 mol l to 1×10 mol l , with a limit detection of-7-1 2×10 mol l. The mean recovery rates for spike recovery experiments varied from 92.0 % to 109 %. A new Schiff base was synthesized from quinoline-2-formaldehyde and βmercaptoethylamine. Then, a new magnetic metal-organic framework Fe O @Fe-ASB was synthesized by 3 4 it with nano-Fe O as the metal center. The Fe O @Fe-ASB was successfully used for the detection of 3 4 3 4 ribavirin in water samples as catalytic material. It showed good stability, accuracy and precision. Catalyst, Feed, Optical chemical sensor, Ribavirin Detection of ribavirin in environmental and biological samples via optical chemical sensor