Selection Signatures in Four Lignin Genes from Switchgrass Populations Divergently Selected for In Vitro Dry Matter Digestibility

Chen, Shiyu; Kaeppler, Shawn M.; Vogel, Kenneth P.; Casler, Michael D.

doi:10.1371/journal.pone.0167005

Cited by 6 publications

(3 citation statements)

References 66 publications

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“…The findings of the study suggested that the extent of substitution of the xylan backbone of hemicellulosic polysaccharides by arabinose (DHS), the degree of xylan acetylation (DHA) and feruloyation (DHF), and the ratio of para-coumaric acid to acid detergent lignin (pCA/ADL) were important areas that can be exploited to obtain greater quality of substrate for bioethanol production. Similarly, Chen et al (2016) also detected specific loci for different switchgrass populations, for in vitro dry matter digestibility and ethanol yield. The study successfully discovered potential markers that can be utilised for breeding switchgrass varieties that can yield higher ethanol titres.…”

Section: Improvements In Bioenergy Crops Through Breeding Marker-assmentioning

confidence: 85%

Engineering grass biomass for sustainable and enhanced bioethanol production

Mohapatra

Mishra

Bhalla

et al. 2019

Planta

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Main conclusion Bioethanol from lignocellulosic biomass is a promising step for the future energy requirements. Grass is a potential lignocellulosic biomass which can be utilised for biorefinery-based bioethanol production. Grass biomass is a suitable feedstock for bioethanol production due to its all the year around production, requirement of less fertile land and noninterference with food system. However, the processes involved, i.e. pretreatment, enzymatic hydrolysis and fermentation for bioethanol production from grass biomass, are both time consuming and costly. Developing the grass biomass in planta for enhanced bioethanol production is a promising step for maximum utilisation of this valuable feedstock and, thus, is the focus of the present review. Modern breeding techniques and transgenic processes are attractive methods which can be utilised for development of the feedstock. However, the outcomes are not always predictable and the time period required for obtaining a robust variety is generation dependent. Sophisticated genome editing technologies such as synthetic genetic circuits (SGC) or clustered regularly interspaced short palindromic repeats (CRISPR) systems are advantageous for induction of desired traits/heritable mutations in a foreseeable genome location in the 1st mutant generation. Although, its application in grass biomass for bioethanol is limited, these sophisticated techniques are anticipated to exhibit more flexibility in engineering the expression pattern for qualitative and qualitative traits. Nevertheless, the fundamentals rendered by the genetics of the transgenic crops will remain the basis of such developments for obtaining biorefinery-based bioethanol concepts from grass biomass.

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Section: Improvements In Bioenergy Crops Through Breeding Marker-assmentioning

confidence: 85%

Engineering grass biomass for sustainable and enhanced bioethanol production

Mohapatra

Mishra

Bhalla

et al. 2019

Planta

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“…Conventional breeding techniques advance our understanding toward marker-assisted selection for biomass-related traits, stress tolerance, and scarification in biofuel grasses and woody plants. In switchgrass, marker-assisted breeding enabled the understanding of substitution of cell wall hemicellulose polymers backbone and remodeling ( De Souza et al, 2015 ) whereas identification of specific loci was identified from potential markers for high ethanol generation from switchgrass populations ( Chen et al, 2016 ). Prairie cordgrass is a potential C 4 bioenergy crop, and two clones of prairie cordgrass were crossed and developed SSR markers for marker-assisted selection of biomass traits ( Gedye et al, 2012 ).…”

Section: Tools and Strategies To Enhance Biomassmentioning

confidence: 99%

Genetic Determinants of Biomass in C4 Crops: Molecular and Agronomic Approaches to Increase Biomass for Biofuels

et al. 2022

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Bio-based fuels have become popular being efficient, cost-effective, and eco-friendly alternatives to fossil fuels. Among plant sources exploited as feedstocks, C4 grasses, such as sugarcane, maize, sorghum, and miscanthus, are highly resourceful in converting solar energy into chemical energy. For a sustainable and reliable supply of feedstocks for biofuels, we expect dedicated bioenergy crops to produce high biomass using minimum input resources. In recent years, molecular and genetic advancements identified various factors regulating growth, biomass accumulation, and assimilate partitioning. Here, we reviewed important genes involved in cell cycle regulation, hormone dynamics, and cell wall biosynthesis. A number of important transcription factors and miRNAs aid in activation of important genes responsible for cell wall growth and re-construction. Also, environmental components interacting with genetic controls modulate plant biomass by modifying gene expression in multiple interacting pathways. Finally, we discussed recent progress using hybridization and genome editing techniques to improve biomass yield in C4 grasses. This review summarizes genes and environmental factors contributing biomass yield in C4 biofuel crops which can help to discover and design bioenergy crops adapting to changing climate conditions.

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“…The genotypic effects for ADF, IVDT, NDFD, and starch content were significant, so it should be possible to improve the DE content of barleybased forage feedstuff. Work in other species, such as that of Vogel (2013) and Chen, Kaeppler, Vogel, and Cassler (2016) in switchgrass (Panicum virgatum L.) and Li et al (2011) in alfalfa (Medicago sativa L.), has shown that it is possible to identify both high FAs and improved nutritive value traits. In their review, Capstaff and Miller (2018) noted that the greatest concurrent improvements in FA and nutritional content of forage crops had occurred in Medicago spp., Trifolium spp., Lolium, and Festuca.…”

Section: Improving the Forage Accumulation-nutritive Value Relationshipmentioning

confidence: 99%

Breeding strategies in evaluation of forage barley

et al. 2021

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Producers on the Canadian Prairies grow barley (Hordeum vulgare L.) as a forage crop for ruminants, especially cattle. The purpose of our study was to determine methods to improve our selection of barley lines with superior forage value for ruminants. Data from the Western Cooperative Forage Barley Registration Test were used with permission of the Prairie Recommending Committee for Oat and Barley (PRCOB) and the breeders. Data were analyzed from 20 environments collected from 3 yr (2012–2014) and seven sites. We measured forage accumulation (FA) at the soft dough stage, five nutritive value traits, and three derived cow–calf variables. Although the variation in FA and forage nutritive value traits attributed to environment (E) was large, significant genotypic (G) effects were found for all traits measured. Whereas G× E effects were also significant, the mean square effect for G was up to 40 times higher than the mean squares for G×E. The proportion of variance attributed to G was greater for nutritive value traits than for FA. Significance of G means there was variability in the germplasm tested, and improvement through selection should be possible. The two parametric methods of stability analyses (Francis–Kannenberg method and Eberhart–Russell method) often identified the same lines as superior. Lines that were superior for FA and carrying capacity (cow on swath grazing, CC) were often different from those identified as superior for nutritive value, empty body weight gain (cow, EBWG) and average daily gain (backgrounding calf, ADG). A nonparametric method used to determine dynamic stability (Nassar–Hühn method) identified different lines as superior from the parametric methods. Breeders can use a combination of these selection criteria in breeding forage barley varieties with enhanced forage value for ruminants.

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Selection Signatures in Four Lignin Genes from Switchgrass Populations Divergently Selected for In Vitro Dry Matter Digestibility

Cited by 6 publications

References 66 publications

Engineering grass biomass for sustainable and enhanced bioethanol production

Engineering grass biomass for sustainable and enhanced bioethanol production

Genetic Determinants of Biomass in C4 Crops: Molecular and Agronomic Approaches to Increase Biomass for Biofuels

Breeding strategies in evaluation of forage barley

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