Selective DNA Adsorption on Layered Double Hydroxide Nanoparticles

Abstract

We investigated the selective deoxyribonucleic acid (DNA) adsorption on layered double hydroxide (LDH) nanoparticles via studying the interaction between positively charged LDH nanoparticle as adsorbent and negatively charged adsorbates such as methyl orange (MO), fluorescein (FL), and DNA strands. The size controlled LDH (Mg0.78Al0.22(OH)2(CO3)0.11 · mH2O) was prepared by conventional coprecipitation method, followed by the hydrothermal treatment. According to the adsorption isotherms, the adsorbed amounts of MO and FL were similar, however, that of DNA were much larger. The adsorption behaviors were well fitted to Freundlich adsorption model. The concentration dependent adsorption behavior on LDH surface was described in order to verify the selective DNA separation ability. The result showed that the LDH has advantages in selective adsorption of DNA competing with single molecular anions.

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“…The pristine MgAl-LDH was prepared by a conventional coprecipitation method followed by hydrothermal treatment, as reported elsewhere ( Kim et al, 2011 ). Mg(NO 3 ) 2 ⋅6H 2 O (0.06 mol) and Al(NO 3 ) 3 ⋅9H 2 O (0.03 mol) were dissolved in 200 mL of DW, and the solution was titrated to pH ∼9.5 with NaOH/NaHCO 3 (0.23 mol/0.17 mol in 250 mL DW).…”

Two-dimensional ultrathin nanosheets of Ni–In-layered double hydroxides prepared in water: enhanced performance for DNA adsorption

“…Chan et al 19 have immobilized LDHs on the polycarbonate substrate as the media to extract the specic DNA molecules through a uidic system, and the results show that the immobilized LDHs extract the specic DNA molecules from the biomolecular solution with high efficiency. Kim et al 20 investigated the selective DNA adsorption on LDH nanoparticles, and they found that the LDHs have advantages in the selective adsorption of DNA. As DNA is highly negatively charged, the adsorption of DNA on positively charged LDHs surfaces is based on electrostatic interactions.…”

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