Selenocysteine detection and bioimaging in living cells by a colorimetric and near-infrared fluorescent turn-on probe with a large stokes shift

A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

“…The response of YZ-A4 to Sec was completed to produce red fluorescence within about 3 min, and the fluorescence enhancement at 614 nm was 20 fold under simulated physiological conditions. The quantitative detection range of the sensor YZ-A4 to Sec was from 0 to 32 μM, and the detection limit was as low as 11.2 nM, whose detection limit was lower and the response time was faster than those of the reported sensors [ 12 , 31 ]. The sensor YZ-A4 exhibited good selectivity to Sec, whereas the biothiols and other amino acids hardly exhibited interference.…”

“…As the reactive site of SeP, Sec content is closely related to the high catalytic capacity of SeP and many physiological functions such as cancer, cardiovascular disease, diabetes, neurodegenerative disease, and male infertility [ 9 , 10 , 11 ]. Due to the important role of Sec in human physiological function, it is necessary to develop a fast and reliable method to detect Sec in biological systems [ 12 ].…”

“…At the same time, based on luciferin, coumarin, hemicyanine, and xanthene dyes, many fluorescent sensors were developed to detect Sec [ 17 , 21 , 22 , 23 , 24 ]. In the design process, fluorescent sensors with long-wavelength emission are preferred to reduce phototoxicity and increase tissue penetration [ 12 , 18 ].…”

Fluorescent probes based on nucleophilic aromatic substitution reactions for reactive sulfur and selenium species: Recent progress, applications, and design strategies

“…A fluorescent probe ( Fig. 5 , panel 12 ) for detecting Sec was synthesized by introducing 2,4-dinitrophenyl ether into a benzopyranonitrile fluorophore [171] . This probe was used to detect Sec in bovine serum samples and exogenous and endogenous Sec in live cells.…”

Unity Makes Strength: How Aggregation‐Induced Emission Luminogens Advance the Biomedical Field

“…An ideal fluorescent agent should have low toxicity, high brightness, excellent stability, good selectivity, and versatile functionality. To date, a vast variety of synthetic fluorescent materials, including organic dyes, fluorescent proteins, inorganic quantum dots (QDs), conjugated polymer nanoparticles (NPs), metallic nanoclusters, as well as upconversion NPs, have been explored and demonstrated for biological applications.…”